An Eight-Parent Multiparent Advanced Generation Inter-Cross Population for Winter-Sown Wheat: Creation, Properties, and Validation

MAGIC populations represent one of a new generation of crop genetic mapping resources combining high genetic recombination and diversity. We describe the creation and validation of an eight-parent MAGIC population consisting of 1091 F7 lines of winter-sown wheat (Triticum aestivum L.). Analyses based on genotypes from a 90,000-single nucleotide polymorphism (SNP) array find the population to be well-suited as a platform for fine-mapping quantitative trait loci (QTL) and gene isolation. Patterns of linkage disequilibrium (LD) show the population to be highly recombined; genetic marker diversity among the founders was 74% of that captured in a larger set of 64 wheat varieties, and 54% of SNPs segregating among the 64 lines also segregated among the eight founder lines. In contrast, a commonly used reference bi-parental population had only 54% of the diversity of the 64 varieties with 27% of SNPs segregating. We demonstrate the potential of this MAGIC resource by identifying a highly diagnostic marker for the morphological character "awn presence/absence" and independently validate it in an association-mapping panel. These analyses show this large, diverse, and highly recombined MAGIC population to be a powerful resource for the genetic dissection of target traits in wheat, and it is well-placed to efficiently exploit ongoing advances in phenomics and genomics. Genetic marker and trait data, together with instructions for access to seed, are available at http://www.niab.com/MAGIC/.

• Publication year

  2014

• Venue

  G3: Genes, Genomes, Genetics

• Publication date

  2014-09-01

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology

• Identifiers
  DOI 10.1534/g3.114.012963PMID 25237112PMCID 4169152
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Multiple Quantitative Trait Analysis Using Bayesian Networks

  2014influential reference
• Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array

  2014cited by this paper
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  2012cited by this paper
• Ten Years of the Collaborative Cross

  2012cited by this paper
• A multiparent advanced generation inter-cross population for genetic analysis in wheat.

  2012influential reference
• Sequence-based marker development in wheat: advances and applications to breeding.

  2012cited by this paper
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  2012cited by this paper
• Genome-wide association mapping to candidate polymorphism resolution in the unsequenced barley genome

  2010cited by this paper
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  2010influential reference
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  2010cited by this paper
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  2010influential reference
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  2009influential reference
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  2008cited by this paper
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• Methods for linkage disequilibrium mapping in crops.

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  2006influential reference
• A method for fine mapping quantitative trait loci in outbred animal stocks.

  2000cited by this paper
• RFLP mapping of the three major genes, Vrn1, Q and B1, on the long arm of chromosome 5A of wheat

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• Catalogue of gene symbols for wheat

  1998cited by this paper
• Microprep protocol for extraction of DNA from tomato and other herbaceous plants

  1995cited by this paper
• Advanced intercross lines, an experimental population for fine genetic mapping.

  1995cited by this paper
• Variation and genetics of the awn inTriticum

  1940cited by this paper
• Bioinformatics Applications Note Genetics and Population Analysis R/mpmap: a Computational Platform for the Genetic Analysis of Multiparent Recombinant Inbred Lines

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