Introduction of H2C2‐type zinc‐binding residues into HIV‐2 Vpr increases its expression level

Human immunodeficiency virus type 2 has two structurally similar proteins, Vpx and Vpr. Vpx degrades the host anti‐viral protein SAMHD1 and is expressed at high levels, while Vpr is responsible for cell cycle arrest and is expressed at much lower levels. We constructed a Vpr mutant with a high level of expression by replacing the amino acids HHCR/HHCH with a putative H2C2‐type zinc‐binding site that is carried by Vpx. Our finding suggests that during the evolution of Vpr and Vpx, zinc‐binding likely became a mechanism for regulating their expression levels.

• Publication year

  2017

• Venue

  FEBS Open Bio

• Publication date

  2017-12-19

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/2211-5463.12358PMID 29321964PMCID 5757179
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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