Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing

No abstract is available for this paper.

• Publication year

  2007

• Venue

  Nature Protocols

• Publication date

  2007-10-01

• Fields of study

  Biology, Medicine, Chemistry, Engineering

• Identifiers
  DOI 10.1038/nprot.2007.362PMID 17947984
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Linear-after-the-exponential polymerase chain reaction and allied technologies. Real-time detection strategies for rapid, reliable diagnosis from single cells.

  2007cited by this paper
• ANALYTICAL BIOCHEMISTRY

  2006cited by this paper
• BMC Biotechnology BioMed Central Methodology article Two-temperature LATE-PCR endpoint genotyping

  2006cited by this paper
• Linear-After-The-Exponential (LATE)-PCR: primer design criteria for high yields of specific single-stranded DNA and improved real-time detection.

  2005cited by this paper
• 指数後の直線(LATE)‐PCR 特異的な一本鎖DNAの高い収量に対するプライマーデザイン基準および改善リアルタイム検出

  2005cited by this paper
• A Practical Approach

  2005cited by this paper
• Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis

  2004cited by this paper
• On-line integration of PCR and cycle sequencing in capillaries: from human genomic DNA directly to called bases.

  2003cited by this paper
• Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR.

  2003cited by this paper
• QuantiLyse: reliable DNA amplification from single cells.

  2002cited by this paper
• ResonSense®: simple linear fluorescent probes for quantitative homogeneous rapid polymerase chain reaction ☆

  2002cited by this paper
• Multiplex polymerase chain reaction: A practical approach

  2002cited by this paper
• Cycle sequencing of PCR products.

  2002cited by this paper
• MELTING, computing the melting temperature of nucleic acid duplex

  2001cited by this paper
• Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.

  2000cited by this paper
• Multiplex PCR: Optimization and Application in Diagnostic Virology

  2000cited by this paper
• Multiplex PCR: critical parameters and step-by-step protocol.

  1997cited by this paper
• Molecular Beacons: Probes that Fluoresce upon Hybridization

  1996cited by this paper
• Convenient single-step, one tube purification of PCR products for direct sequencing.

  1994cited by this paper
• Direct DNA sequencing of PCR-amplified vector inserts following enzymatic degradation of primer and dNTPs.

  1994cited by this paper
• Large-scale production of DNA sequencing templates by microtitre format PCR

  1993cited by this paper
• Direct sequencing of double-stranded PCR products without intermediate fragment purification; digestion with mung bean nuclease.

  1993cited by this paper
• Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications.

  1992cited by this paper
• Rapid and reliable fluorescent cycle sequencing of double-stranded templates.

  1992cited by this paper
• Direct sequencing by thermal asymmetric PCR.

  1991cited by this paper
• Amplification of bacterial genomic DNA by the polymerase chain reaction and direct sequencing after asymmetric amplification: application to the study of periplasmic permeases

  1989cited by this paper
• DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

  1988cited by this paper
• Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

  1988cited by this paper

• Nonlinear Classifiers Based on DNA Logic Circuits for Cancer Diagnosis.

  2025cites this paper
• A spatially localized DNA linear classifier for cancer diagnosis

  2024cites this paper
• An automated DNA computing platform for rapid etiological diagnostics

  2022cites this paper
• Rapid Pyrazinamide Drug Susceptibility Testing using a Closed-Tube PCR Assay of the Entire pncA gene

  2020cites this paper
• Cancer diagnosis with DNA molecular computation

  2020cites this paper
• An innovative oligonucleotide microarray to detect spoilage microorganisms in wine

  2018cites this paper
• Virtual Barcoding using LATE-PCR and Lights-On/Lights-Off probes: identification of nematode species in a closed-tube reaction

  2016cites this paper
• Closed-Tube Barcoding.

  2016cites this paper
• Preparation of Single-Stranded DNA for Pyrosequencing by LATE-PCR

  2016cites this paper
• Pyrosequencing Templates Generated by Nicking PCR Products with Nicking Endonuclease

  2016cites this paper
• Construction of 3-Plex Barcodes for Differential Gene Expression Analysis with Pyrosequencing

  2016cites this paper
• Low-concentration initiator primers improve the amplification of gene targets with high sequence variability.

  2015cites this paper
• On-site detection of Phytophthora spp.—single-stranded target DNA as the limiting factor to improve on-chip hybridization

  2014cites this paper
• Single-Tube Mutation Scanning of The Epidermal Growth Factor Receptor Gene Using Multiplex LATE-PCR and Lights-On/Lights-Off Probes.

  2014influential citation
• AZT treatment increases mtDNA mutations in HepG2 and CCD-1112Sk cells.

  2013cites this paper
• Rapid detection and identification of hepatitis C virus (HCV) sequences using mismatch-tolerant hybridization probes: A general method for analysis of sequence variation.

  2013cites this paper
• Rapid detection of TEM-type extended-spectrum β-lactamase (ESBL) mutations using lights-on/lights-off probes with single-stranded DNA amplification.

  2013cites this paper
• Design and construction of a single tube, quantitative endpoint, LATE‐PCR multiplex assay for ventilator‐associated pneumonia

  2013influential citation
• Design of a single‐tube, endpoint, linear‐after‐the‐exponential‐PCR assay for 17 pathogens associated with sepsis

  2013cites this paper
• Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA.

  2013cites this paper
• Organ damage in HIV-positive patients with high blood pressure.

  2013cites this paper
• Rapid detection of sequence variation in Clostridium difficile genes using LATE-PCR with multiple mismatch-tolerant hybridization probes.

  2012cites this paper
• Fluorescent signatures for variable DNA sequences

  2012cites this paper
• A peroxidase-active aptazyme as an isothermally amplifiable label in an aptazyme-linked oligonucleotide assay for low-picomolar IgE detection.

  2012cites this paper
• Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis

  2012cites this paper
• rotocols esign and verification of a highly reliable Linear-After-The-Exponential PCR LATE-PCR ) assay for the detection of African swine fever virus

  2011cites this paper
• LATE-PCR and allied technologies: real-time detection strategies for rapid, reliable diagnosis from single cells.

  2011cites this paper
• Design and verification of a highly reliable Linear-After-The-Exponential PCR (LATE-PCR) assay for the detection of African swine fever virus.

  2011cites this paper
• Pan-serotypic detection of foot-and-mouth disease virus by RT linear-after-the-exponential PCR.

  2010cites this paper
• Toward routine, DNA-based detection methods for marine pests.

  2010cites this paper
• Dilute-‘N’-Go dideoxy sequencing of all DNA strands generated in multiplex LATE-PCR assays

  2010influential citation
• One-step RT-LATE-PCR for mRNA and viral RNA detection and quantification.

  2010cites this paper
• Pyrosequencing on nicked dsDNA generated by nicking endonucleases.

  2010cites this paper
• Heat shock memory in preimplantation mouse embryos.

  2010cites this paper
• DNA methylation analysis on a droplet-in-oil PCR array.

  2009cites this paper
• Preparation of Single-Stranded DNA for Pyrosequencing by Linear-after-the-Exponential-Polymerase Chain Reaction

  2009cites this paper
• Dye-free gene expression detection by sequence-tagged reverse-transcription polymerase chain reaction coupled with pyrosequencing.

  2009cites this paper
• Single-Molecule LATE-PCR Analysis of Human Mitochondrial Genomic Sequence Variations

  2009cites this paper
• Design and optimization of a novel reverse transcription linear‐after‐the‐exponential PCR for the detection of foot‐and‐mouth disease virus

  2009cites this paper
• Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos

  2007cites this paper