Rat alpha 1-cysteine proteinase inhibitor. An acute phase reactant identical with alpha 1 acute phase globulin.

alpha 1-Cysteine proteinase inhibitor was isolated from normal and acute phase rat serum. The procedure, which includes successive fractionations on AcA 34, Cibacron blue Sepharose, DEAE-Sephacel, and hydroxyapatite, but avoids the use of an affinity chromatography step on a cysteine proteinase gel, led to the preparation of two electrophoretically distinct components. These resolved to a single band of apparent Mr = 68,000 when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A slightly higher Mr was estimated from gel filtration studies, possibly related to the rather high carbohydrate content (15.25% of the dry weight). The purified protein exhibited strong inhibiting capacity towards papain with a Ki of 5 X 10(-11) M. As shown by immunochemical quantitation as well as functional activity, a 6-10-fold increase in the alpha 1-cysteine cysteine proteinase inhibitor content was recorded in inflammatory serum, thus demonstrating the protein to be a typical acute phase reactant. Its partial physicochemical characterization (Mr, isoelectric point, extinction coefficient, amino acid and carbohydrate compositions) shows large similarities with alpha 1 acute phase globulin whose biological function remains unknown, and which is present at the same concentration (about 0.5 g/liter) in normal serum. Identity between the two molecules has been further demonstrated by double immunodiffusion analysis since a continuous precipitating line was observed when purified alpha 1-cysteine proteinase inhibitor was reacted against anti-alpha 1 acute phase globulin and anti-alpha 1-cysteine cysteine proteinase inhibitor antibodies.

• Publication year

  1983

• Venue

  Journal of Biological Chemistry

• Publication date

  1983-10-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(17)44195-0PMID 6195153
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• In vitro method for testing the toxin-producing capacity of diphtheria bacteria.

  2009cited by this paper
• A heat stable low molecular weight inhibitor of lysosomal cysteine proteinases in human serum.

  1983cited by this paper
• Comparison of properties of thiol proteinase inhibitors from rat serum and liver.

  1982cited by this paper
• Low molecular weight inhibitors of cathepsins B, H and T in human serum, synovial fluid and CSF.

  1982cited by this paper
• Existence of two kinds of thiol protease inhibitors in rat serum.

  1982cited by this paper
• L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L.

  1982cited by this paper
• Assay of alpha-cysteine proteinase inhibitor in serum or plasma.

  1982cited by this paper
• THE PHENOMENON OF THE ACUTE PHASE RESPONSE *

  1982cited by this paper
• Changes in the blood level and affinity to concanavalin A of rat plasma glycoproteins during acute inflammation and hepatoma growth.

  1982cited by this paper
• A new pentraxin (serum amyloid P-component) in the rat: evidence for two quaternary structures and effect of ligands on self-association.

  1981cited by this paper
• Multimolecular forms of thiol proteinase inhibitor in human plasma.

  1981cited by this paper
• Cathepsin B, Cathepsin H, and cathepsin L.

  1981cited by this paper
• Purification and some characteristics of two human serum proteins inhibiting papain and other thiol proteinases

  1979cited by this paper
• A rat serum glycoprotein whose synthesis rate increases greatly during inflammation.

  1979cited by this paper
• Isolation and partial characterisation of a thiol proteinase inhibitor from human plasma.

  1979cited by this paper
• A single-column amino acid analysis method which resolves hexosamines and several cysteine derivatives.

  1978cited by this paper
• A new serum component which specifically inhibits thiol proteinases.

  1977cited by this paper
• Rat alpha2 acute-phase macroglobulin. Isolation and physicochemical properties.

  1976cited by this paper
• The alpha macroglobulins of rat serum.

  1976cited by this paper
• Studies on acute phase proteins of rat serum. II. Determination of the contents of 1 -acid glycoprotein, 2-macroglobulin, and albumin in serum from rats suffering from induced inflammation.

  1972cited by this paper
• 1 -Acute-phase globulins of rats. Microheterogeneity after isoelectric focusing.

  1972cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Alpha-1 acute-phase globulins of rats. Isolation and some properties.

  1969cited by this paper
• RESPONSE OF A SERUM GLYCOPROTEIN TO TISSUE INJURY AND NECROSIS. I. THE RESPONSE TO NECROSIS, HYPERPLASIA AND TUMOUR GROWTH.

  1964cited by this paper
• DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS *

  1964cited by this paper
• Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.

  1961cited by this paper

• Structure determinants defining the specificity of papain-like cysteine proteases

  2022cites this paper
• Rat cathepsin K: Enzymatic specificity and regulation of its collagenolytic activity.

  2019cites this paper
• Comparación de las proteínas de fase aguda expresadas en suero de ratón por infección de referencia con Brucella suis y una nueva cepa emergente aislada de ranas

  2015cites this paper
• Régulation de l'activité de la protéine du surfactant SP-A par les cathepsines à cystéine pulmonaires : conséquences sur les propriétés antibactériennes de SP -A.

  2011cites this paper
• Kininogens: More than cysteine protease inhibitors and kinin precursors.

  2010cites this paper
• Investigation of chiralities of propranolol and atenolol by comparative proteomic analysis of vascular smooth muscle cells.

  2009cites this paper
• Comparative proteomic analysis of extracellular proteins reveals secretion of T-kininogen from vascular smooth muscle cells in response to incubation with s-enantiomer of propranolol.

  2008cites this paper
• Lung cysteine cathepsins: intruders or unorthodox contributors to the kallikrein-kinin system?

  2008cites this paper
• Modulation of hypotensive effects of kinins by cathepsin K.

  2007cites this paper
• Serum neutrophil elastase levels predict initial clinical condition but do not correlate with the progression of interstitial lung disease.

  2003cites this paper
• Kininogen-derived fluorogenic substrates for investigating the vasoactive properties of rat tissue kallikreins--identification of a T-kinin-releasing rat kallikrein.

  1997cites this paper
• Interactions between ovine cathepsin L, cystatin C and alpha 2-macroglobulin. Potential role in the genital tract.

  1997cites this paper
• A role for B1 and B2 kinin receptors in the modulation of T-kininogen during the acute phase response of inflammation.

  1996cites this paper
• Trypanosomatid cysteine protease activity may be enhanced by a kininogen-like moiety from host serum.

  1995cites this paper
• Purification and characterization of acute phase rat plasma thiostatin.

  1994cites this paper
• Hepatocyte and immune system: acute phase reaction as a contribution to early defence mechanisms.

  1994cites this paper
• HNF4 and HNF1 as well as a panel of hepatic functions are extinguished and reexpressed in parallel in chromosomally reduced rat hepatoma-human fibroblast hybrids

  1993cites this paper
• Immunological characterization of rat kininogens with monoclonal antibodies to T-kininogen. Distinction between the different domains of T-kininogen and the multiple rat kininogens.

  1992cites this paper
• Comparative study of asparagine-linked glycans of plasma T-kininogen in normal rats and during acute inflammation.

  1992cites this paper
• Differential rates of glycoprotein secretion by isolated rat hepatocytes studied in terms of concanavalin A binding.

  1992cites this paper
• Measurement of T-kinin in rat plasma using a specific radioimmunoassay.

  1992cites this paper
• Biochemical and physiological studies on two T-kininogen species using monoclonal antibodies.

  1992cites this paper
• Regulation of C4b-binding protein gene expression by the acute-phase mediators tumor necrosis factor-alpha, interleukin-6, and interleukin-1.

  1992cites this paper
• The kallikrein-kinin system in the rat hypothalamus

  1991cites this paper
• Discrimination between rat thiostatin (T-kininogen) and one of its cystatin-like inhibitory fragments by a monoclonal antibody, and localization of the epitope.

  1991cites this paper
• Quantification of acute phase proteins in rat serum and in the supernatants of a cultured rat hepatoma cell line and cultured primary hepatocytes by an enzyme-linked immunosorbent assay.

  1990cites this paper
• Differential regulation of rat T-kininogen by tumor necrosis factor and interleukin-6.

  1990cites this paper
• Nutritionally-induced elevation of serum proteinase inhibitors and reduced 3-methylhistidine excretion in the rat.

  1990cites this paper
• Immunologic methods for quantitative estimation of small peptides and their application to bradykinin.

  1990cites this paper
• Identification of a Protein Increasing in Serum of Nagase Analbuminemic Rats Bearing Intestinal Tumors as an Isotype of T‐Kininogen

  1990cites this paper
• Effect of turpentine on alpha 1-major acute phase concentration in normal and adrenalectomized rats.

  1990cites this paper
• Inflammatory competence of fetal rat: acute-phase plasma protein response of the fetus treated by turpentine in utero.

  1989cites this paper
• Control of kininogen gene expression.

  1989cites this paper
• Limited proteolysis of T-kininogen (thiostatin). Release of comparable fragments by different endopeptidases.

  1989cites this paper
• Inflammatory competence of fetal rat

  1989cites this paper
• Isolation and partial characterization of a novel form of low molecular weight kininogen from guinea pig plasma.

  1989cites this paper
• Synthesis of thiostatins (major acute-phase alpha 1 proteins) in different strains of Rattus norvegicus.

  1989cites this paper
• Purification and characterization of plasma T-kininogen from Wistar and brown Norway rats.

  1989cites this paper
• An enzyme-linked immunosorbent assay (ELISA) for kinins in tryptic digests.

  1989cites this paper
• Quantitative determination of kinins released by trypsin using enzyme-linked immunosorbent assay (ELISA) and identification by high-performance liquid chromatography (HPLC).

  1989cites this paper
• The Acute Phase Response in the Rodent a

  1989cites this paper
• Kininogen in tumor-bearing mice.

  1989cites this paper
• Acute Phase Biosynthesis of Kininogens by Livers from Normal and Turpentine‐treated Rats a

  1989cites this paper
• Glucocorticoid and estrogen regulation of a rat T-kininogen gene.

  1989cites this paper
• Effect of thyroidectomy on rat plasma T-kininogen concentration as demonstrated by a new and direct T-kininogen radioimmunoassay.

  1989cites this paper
• Differential effects of inflammation models on rat T-kininogen and rat angiotensinogen.

  1988cites this paper
• Pig plasma protease inhibitor gene complex: isolation and partial characterization of three inhibitors.

  1988cites this paper
• Effect of dexamethasone on kininogen production by a rat hepatoma cell line.

  1988cites this paper
• Purification and characterization of rat T-kininogens isolated from plasma of adjuvant-treated rats. Identification of three kinds of T-kininogens.

  1988cites this paper
• T-kinin release from T-kininogen by rat-submaxillary-gland endopeptidase K.

  1988cites this paper
• Purification and characterization of two kinds of low molecular weight kininogens from rat (non-inflamed) plasma. One resistant and the second sensitive to rat glandular kallikreins.

  1988cites this paper
• Effect of thyroidectomy on rat T-kininogen.

  1988cites this paper
• Changes in proteinase/proteinase inhibitor levels in rat skeletal muscle tissue during diabetes and fasting.

  1988cites this paper
• Acute phase response of plasma proteins in analbuminemic rats.

  1988cites this paper
• Differential effects of nephrectomy and surgery on plasma kininogens and angiotensinogen in the rat.

  1988cites this paper
• Cysteine-proteinase-inhibiting function of T kininogen and of its proteolytic fragments.

  1988cites this paper
• Proteinase inhibitors as acute phase reactants: regulation of synthesis and turnover.

  1988cites this paper
• Purification and characterization of α1-thiol proteinase inhibitor and its identity with kinin and fragment 1.2 free high molecular weight kininogen

  1988cites this paper
• Possible relationship between the restricted biological function of rat T kininogen (thiostatin) and its behaviour as an acute phase reactant.

  1988cites this paper
• Comparison of the acute phase response of cultured Morris hepatoma 7777 cells and of rat hepatocytes.

  1987cites this paper
• Role of High Molecular Weight Kininogen in Contact Activation

  1987cites this paper
• Limited effects of recombinant human and murine interleukin 1 and tumour necrosis factor on production of acute phase proteins by cultured rat hepatocytes.

  1987cites this paper
• Purification of the Ca2+-dependent proteinase inhibitor from bovine cardiac muscle and its interaction with the millimolar Ca2+-dependent proteinase.

  1987cites this paper
• Three high molecular weight protease inhibitors of rat plasma. Isolation, characterization, and acute phase changes.

  1987cites this paper
• Substance P precursor and kininogen: their structures, gene organizations, and regulation.

  1987cites this paper
• [Molecular mechanism for gene expression in kininkallikrein system].

  1987cites this paper
• Structure and expression of the genes for major acute phase alpha 1-protein (thiostatin) and kininogen in the rat.

  1987cites this paper
• Novel Functions of the Kininogens

  1987cites this paper
• 5 – Synthesis, Processing, and Secretion of Plasma Proteins by the Liver and Other Organs and Their Regulation

  1987cites this paper
• The novel rat kininogen, T-kininogen. A pro-inflammatory factor or a thiostatin?

  1987cites this paper
• Direct radioimmunoassay for rat T-kininogen.

  1987cites this paper
• Inhibition of chicken calpain II by proteins of the cystatin superfamily and alpha 2-macroglobulin.

  1987cites this paper
• Thiol proteinase inhibitor in the ascitic fluid of sarcoma 180 tumor-bearing mice.

  1987cites this paper
• T-kinin and T-kininogen--an historical overview.

  1986cites this paper
• Purification of two species of exudate cysteine‐proteinase inhibitors that are acute‐phase reactants in the carrageenin‐induced inflammation in rats

  1986cites this paper
• Acute phase plasma proteins in kininogen-deficient Brown Norway rats.

  1986cites this paper
• Haptoglobin and proteinase inhibitors in the blood serum of women with inflammatory, benign and neoplastic lesions of the ovary.

  1986cites this paper
• The cystatins: a diverse superfamily of cysteine peptidase inhibitors.

  1986cites this paper
• Relationship between the cysteine-proteinase-inhibitory function of rat T kininogen and the release of immunoreactive kinin upon trypsin treatment.

  1986cites this paper
• Modulation of alpha 2-acute phase globulin serum concentration in turpentine- and immune complex-challenged rats by dexamethasone and adrenaline: evidence for a synergistic mode of action.

  1986cites this paper
• Alpha-1-acute phase globulin in the blood of tunicamycin-injected rats. Isolation of the non-glycosylated form, its inhibitory properties and synthesis in liver slices.

  1986cites this paper
• T-kininogen--the major plasma kininogen in rat adjuvant arthritis.

  1985cites this paper
• Biosynthesis and secretion of α1 acute‐phase globulin in primary cultures of rat hepatcytes

  1985cites this paper
• Structure, Regulation and Evolution of the Genes for the Renin-Angiotensin and the Kallikrein-Kinin Systems

  1985cites this paper
• Biosynthesis and secretion of alpha 1 acute-phase globulin in primary cultures of rat hepatocytes.

  1985cites this paper
• Murine interleukin 1 stimulates α2‐macroglobulin synthesis in rat hepatocyte primary cultures

  1985cites this paper
• Acute-phase response of plasma protein synthesis during experimental inflammation in neonatal rats

  1985cites this paper
• Purification and characterization of an inhibitor of the cysteine protease from the hemolymph of Sarcophaga peregrina larvae.

  1985cites this paper
• Differential expression of the multiple forms of rat prekininogen mRNAs after acute inflammation.

  1985cites this paper
• Plasma proteinase inhibitors in Morris hepatoma-bearing rats: changes in the blood level and synthesis in tissue slices.

  1985cites this paper
• A new function of kininogens as thiol‐proteinase inhibitors: inhibition of papain and cathepsins B, H and L by bovine, rat and human plasma kininogens

  1985cites this paper
• Rat plasma alpha 1-inhibitor3: a member of the alpha-macroglobulin family.

  1985cites this paper
• Major acute-phase alpha(1)protein in the rat: structure, molecular cloning, and regulation of mRNA levels.

  1985cites this paper
• Primary structures of the mRNAs encoding the rat precursors for bradykinin and T-kinin. Structural relationship of kininogens with major acute phase protein and alpha 1-cysteine proteinase inhibitor.

  1985cites this paper
• Major acute phase α1‐protein of the rat is homologous to bovine kininogen and contains the sequence for bradykinin: its synthesis is regulated at the mRNA level

  1985cites this paper
• Murine interleukin 1 stimulates alpha 2-macroglobulin synthesis in rat hepatocyte primary cultures.

  1985cites this paper
• Molecular basis of intracellular regulation of thiol proteinase inhibitors.

  1985cites this paper
• Changes of the level of proteinase inhibitors in rat plasma during turpentine-induced inflammation

  1985cites this paper
• The relationship between rat major acute phase protein and the kininogens.

  1985cites this paper
• More homologies among the vertebrate plasma proteins

  1985cites this paper