Titering lentiviral vectors: comparison of DNA, RNA and marker expression methods

No abstract is available for this paper.

• Publication year

  2002

• Venue

  Gene Therapy

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/sj.gt.3301731PMID 12170379
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Lentiviral Vectors

  2002cited by this paper
• Packaging cell line DNA contamination of vector supernatants: implication for laboratory and clinical research.

  2001cited by this paper
• Functional correction of fanconi anemia group C hematopoietic cells by the use of a novel lentiviral vector.

  2001cited by this paper
• Lentiviral vectors: excellent tools for experimental gene transfer and promising candidates for gene therapy

  2000cited by this paper
• Development of lentiviral vectors for gene therapy for human diseases.

  2000cited by this paper
• Lentiviral vectors.

  2000cited by this paper
• Rapid titer determination using quantitative real-time PCR

  1999cited by this paper
• Lentivirus Vectors Using Human and Simian Immunodeficiency Virus Elements

  1999cited by this paper
• Use of enhanced green fluorescent protein to optimize and quantitate infection of target cells with recombinant retroviruses.

  1999cited by this paper
• A Third-Generation Lentivirus Vector with a Conditional Packaging System

  1998cited by this paper
• Retroviral Particles Produced from a Stable Human-Derived Packaging Cell Line Transduce Target Cells with Very High Efficiencies

  1997cited by this paper
• A rapid screening procedure for the identification of high-titer retrovirus packaging clones

  1997cited by this paper
• A Simple and Reliable Method for Screening Retroviral Producer Clones Without Selectable Markers

  1997cited by this paper
• Rapid retrovirus titration using competitive polymerase chain reaction.

  1996cited by this paper
• In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector

  1996cited by this paper
• Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells

  1996cited by this paper
• Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector.

  1996cited by this paper
• Proteoglycans secreted by packaging cell lines inhibit retrovirus infection

  1996cited by this paper
• Effects of retroviral vector design on expression of human adenosine deaminase in murine bone marrow transplant recipients engrafted with genetically modified cells.

  1995cited by this paper
• Cycles, chaos, and evolution in virus cultures: a model of defective interfering particles.

  1994cited by this paper
• Retrovirus Packaging Cells

  1990cited by this paper

• Challenges and Opportunities in Lentivirus Viral Vector Manufacturing for In Vivo Applications.

  2026cites this paper
• Vesicular Stomatitis Virus Glycoprotein- and Lentiviral RNA-Based Robust Approach for Rapid and Accurate Quantification of Viable Lentiviral Vectors.

  2025cites this paper
• Lymph-Node Inspired Hydrogels Enhance CAR Expression and Proliferation of CAR T Cells

  2025cites this paper
• IL-13 protects epithelial cells from SARS-CoV-2 infection by inhibiting ACE2-mediated virus binding and cell entry

  2025cites this paper
• Optimizing activation and culture conditions for the production of cord blood‐derived CAR‐T and natural killer cells

  2025cites this paper
• Utilizing shRNA-expressing lentivectors for viral hemorrhagic septicemia virus suppression via NV gene targeting

  2025cites this paper
• Foxg1 bimodally tunes L1-mRNA and -DNA dynamics in the developing murine neocortex

  2024cites this paper
• Effects of an indole derivative on cell proliferation, transfection, and alternative splicing in production of lentiviral vectors by transient co-transfection

  2024cites this paper
• Development and validation of a droplet digital PCR method for quantifying lentiviral vector infectious titer

  2024cites this paper
• ANGPTL4 is a potential driver of HCV-induced peripheral insulin resistance

  2023cites this paper
• Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR)

  2023cites this paper
• Foxg1 bimodally tunes L1-mRNA and -DNA dynamics in the developing murine neocortex

  2023cites this paper
• Essential role of an ERV-derived Env38 protein in adaptive humoral immunity against an exogenous SVCV infection in a zebrafish model

  2023cites this paper
• Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

  2022cites this paper
• RNA interference targeting UL25 gene as a gene therapy approach against BHV-1.

  2022cites this paper
• Activation of a hippocampal CREB-pCREB-miRNA-MEF2 axis modulates individual variation of spatial learning and memory capability.

  2021cites this paper
• A guide in lentiviral vector production for hard-to-transfect cells, using cardiac-derived c-kit expressing cells as a model system

  2021cites this paper
• FRugally Optimized DNA Octomer (FRODO) qPCR Measurement of HIV and SIV in Human and Nonhuman Primate Samples

  2021cites this paper
• The OM-85 bacterial lysate inhibits SARS-CoV-2 infection of epithelial cells by downregulating SARS-CoV-2 receptor expression

  2021cites this paper
• Brucella spp. Omp25 Promotes Proteasome-Mediated cGAS Degradation to Attenuate IFN-β Production

  2021cites this paper
• More Than Just Gene Therapy Vectors: Lentiviral Vector Pseudotypes for Serological Investigation

  2021cites this paper
• Platelet derived growth factor-A (Pdgf-a) gene transfer modulates scar composition and improves left ventricular function after myocardial infarction.

  2021cites this paper
• In vitro Inhibition of Border Disease Virus Replication With Lentivirus-Mediated shRNAs

  2021cites this paper
• A Novel Branched DNA-Based Flowcytometric Method for Single-Cell Characterization of Gene Therapy Products and Expression of Therapeutic Genes

  2021cites this paper
• Cytochalasin B-induced membrane vesicles from human mesenchymal stem cells overexpressing TRAIL, PTEN and IFN-β1 can kill carcinoma cancer cells.

  2021cites this paper
• Design and Evaluation of AgoshRNAs with 3'-Terminal HDV Ribozymes to Enhance the Silencing Activity.

  2021cites this paper
• Study on Apoptosis of Prostate Cancer Cells Induced by IκBα Overexpression Synergistic with Poly (Lactic-Co-Glycolic Acid)-Curcumin Nanoparticles

  2021cites this paper
• Lentiviral mediated gene delivery as an effective therapeutic approach for Parkinson disease.

  2021cites this paper
• Selective optogenetic stimulation of efferent fibers in the vagus nerve of a large mammal

  2020cites this paper
• Ex Vivo Rat Transected Spinal Cord Slices as a Model to Assess Lentiviral Vector Delivery of Neurotrophin-3 and Short Hairpin RNA against NG2

  2020cites this paper
• Lentivirus expressing shRNAs inhibit the replication of contagious ecthyma virus by targeting DNA polymerase gene

  2020cites this paper
• Concise review on optimized methods in production and transduction of lentiviral vectors in order to facilitate immunotherapy and gene therapy.

  2020cites this paper
• Long non-coding RNA GRASLND enhances chondrogenesis via suppression of the interferon type II signaling pathway

  2020cites this paper
• Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency

  2020cites this paper
• Efficient engraftment of genetically modified cells is necessary to ameliorate central nervous system involvement of murine model of mucopolysaccharidosis type II by hematopoietic stem cell targeted gene therapy.

  2020cites this paper
• Scaffold‐Mediated Static Transduction of T Cells for CAR‐T Cell Therapy

  2020cites this paper
• ASSESSMENT, AND STREAMLINED PREPARATION OF LOW CYTOTOXICITY LENTIVIRAL VECTORS FOR MOBILIZED HUMAN HEMATOPOIETIC STEM CELL TRANSDUCTION.

  2020cites this paper
• Evaluation of real-time PCR as an alternative for potency testing of Brucella abortus vaccines

  2020cites this paper
• Preclinical Development of Autologous Hematopoietic Stem Cell-Based Gene Therapy for Immune Deficiencies: A Journey from Mouse Cage to Bed Side

  2020cites this paper
• Rapid In-Process Monitoring of Lentiviral Vector Particles by High-Performance Liquid Chromatography

  2020cites this paper
• Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen

  2019cites this paper
• Tubulin detyrosination promotes human trophoblast syncytium formation

  2019cites this paper
• A hysteretic genetic circuit for detection of proteasomal degradation in mammalian cells.

  2019cites this paper
• Long non-coding RNA GRASLND enhances chondrogenesis via suppression of interferon type II signaling pathway

  2019cites this paper
• Metabolic stress activates an ERK/hnRNPK/DDX3X pathway in pancreatic β cells

  2019cites this paper
• Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins

  2019cites this paper
• Transient Lentiviral Vector Production Using a Packed-Bed Bioreactor System

  2019cites this paper
• Production of CAR T-cells by GMP-grade lentiviral vectors: latest advances and future prospects

  2019cites this paper
• Generation of High-Titer Pseudotyped Lentiviral Vectors.

  2019cites this paper
• JUND regulates pancreatic β cell survival during metabolic stress

  2019cites this paper
• Direct genome editing of patient-derived xenografts using CRISPR-Cas9 enables rapid in vivo functional genomics

  2019cites this paper
• Unraveling The Beta Cell Translatome: Elucidation Of An Erk/hnrnpk/jund Axis

  2019cites this paper
• High-affinity T cell receptors redirect cytokine-activated T cells (CAT) to kill cancer cells

  2019cites this paper
• Determination of Lentiviral Infectious Titer by a Novel Droplet Digital PCR Method

  2018cites this paper
• Efficient lentiviral transduction of different human and mouse cells

  2018cites this paper
• Brucella Downregulates Tumor Necrosis Factor-α to Promote Intracellular Survival via Omp25 Regulation of Different MicroRNAs in Porcine and Murine Macrophages

  2018cites this paper
• Light-induced intracellular hydrogen peroxide generation through genetically encoded photosensitizer KillerRed-SOD1

  2018cites this paper
• A Portable Device Integrated with Aligned Carbon Nanotubes for Sensitive Virus Capture and Detection

  2018cites this paper
• b-globin gene transfer to human bone marrow for sickle cell disease

  2018cites this paper
• Scale-up of lentiviral vectors for gene therapy: advances and challenges

  2017cites this paper
• Lentivirus-mediated bta-miR-193 a Overexpression Promotes Apoptosis of MDBK Cells by Targeting BAX and Inhibits BVDV Replication

  2017cites this paper
• King, Barnabas and Temperton, Nigel and Grehan, Keith and Scott, Simon D. and Wright, Edward and Tarr, Alexander W. and Daly, Janet M. (2016) Technical considerations for the generation of novel pseudotyped

  2017cites this paper
• Optimization of Proteasomal Degradation Reporter (eDeg-On) System for CRISPR-mediated Whole-genome Knockout Screens

  2017influential citation
• Strategies and methods for the detection and identification of viral vectors

  2017cites this paper
• The study of highly pathogenic emerging zoonotic virus envelope proteins through pseudotyped virus generation

  2017influential citation
• Lentivirus Aracılı bta-miR-193a Overeksprasyonu MDBK Hücrelerinin Apoptozisini Artırır ve BVDV Replikasyonunu Baskılar

  2017cites this paper
• The Effects of Lentivirus-mediated shRNA Interference Targeting Mcl-1 on Growth of NK/T-cell Lymphoma.

  2017cites this paper
• Reverse Transcription Quantitative Polymerase Chain Reaction for Detection of and Differentiation Between RNA and DNA of HIV-1-Based Lentiviral Vectors

  2017cites this paper
• Suitability of small diagnostic peripheral-blood samples for cell-therapy studies.

  2017cites this paper
• Temporal effect of manipulating NeuroD1 expression with the synthetic small molecule KHS101 on morphine contextual memory

  2017cites this paper
• Interleukin-6 Mediates Post-Infarct Repair by Cardiac Explant-Derived Stem Cells

  2017cites this paper
• Title MECP 2 Is a Frequently Amplified Oncogene with a Novel Epigenetic Mechanism that Mimics the Role of Activated RAS in Malignancy Authors / Affiliation

  2017cites this paper
• Isolation of human explant derived cardiac stem cells from cryopreserved heart tissue

  2017cites this paper
• Differential Regulation of Lipoprotein and Hepatitis C Virus Secretion by Rab1b.

  2017cites this paper
• Mutations dans la région précore du virus de l’hépatite B et fibrose hépatique : approche épidémiologique et application fondamentale

  2017cites this paper
• MicroRNA-539 Inhibits the Epithelial–Mesenchymal Transition of Esophageal Cancer Cells by Twist-Related Protein 1-Mediated Modulation of Melanoma-Associated Antigen A4

  2017cites this paper
• Strategien zum Nachweis und der Identifizierung viraler Vektoren

  2017cites this paper
• Chromatin remodeling gene AT-rich interactive domain-containing protein 1A suppresses gastric cancer cell proliferation by targeting PIK3CA and PDK1

  2016cites this paper
• Differences in Vector Genome Processing and Illegitimate Integration of Non-Integrating Lentiviral Vectors

  2016cites this paper
• Introduction of shRNAs, miRNAs, or AntagomiRs into Primary Human Liver Cells Through Lentiviral Vectors.

  2016cites this paper
• Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates

  2016cites this paper
• Prosurvival Factors Improve Functional Engraftment of Myogenically Converted Dermal Cells into Dystrophic Skeletal Muscle

  2016cites this paper
• Real Time PCR-Based Infectivity Assay and Characterization of Cell Surface Receptors for Turkey Hemorrhagic Enteritis Virus

  2016cites this paper
• Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction.

  2016cites this paper
• Challenges of up-scaling lentivirus production and processing.

  2016cites this paper
• Morphine Modulates Adult Neurogenesis and Contextual Memory by Impeding the Maturation of Neural Progenitors

  2016cites this paper
• A descriptive guide for absolute quantification of produced shRNA pseudotyped lentiviral particles by real-time PCR

  2016influential citation
• Analysis of Hepatocyte Secretion Pathways: A Case Study on Hepatic Apolipoproteins, Serum Albumin, and Hepatitis C Virus

  2016cites this paper
• Kinetics of lentiviral vector transduction in human CD34(+) cells.

  2016cites this paper
• The hepatocyte growth factor-expressing character is required for mesenchymal stem cells to protect the lung injured by lipopolysaccharide in vivo

  2016cites this paper
• Technical considerations for the generation of novel pseudotyped viruses

  2016cites this paper
• Specific promoter deacetylation of histone H3 is conserved across mouse models of Huntington's disease in the absence of bulk changes.

  2016cites this paper
• Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment

  2016cites this paper
• Troubleshooting methods for the generation of novel pseudotyped viruses

  2016cites this paper
• Hepatitis C Virus Increases Occludin Expression via the Upregulation of Adipose Differentiation-Related Protein

  2016influential citation
• Lentiviral vectors titration using real-time PCR

  2015cites this paper
• Ly49 RECEPTORS: REQUIREMENTS FOR LIGAND RECOGNITION AND STRUCTURAL ASSEMBLY

  2015cites this paper
• Antagonism Between Saturated and Unsaturated Fatty Acids in ROS Mediated Lipotoxicity in Rat Insulin-Producing Cells

  2015cites this paper
• Impacts of human LDLR overexpression on the production of a VSVG-pseudotyped lentiviral vector

  2015cites this paper
• Both Foot-and-Mouth Disease Virus and Bovine Viral Diarrhea Virus Replication are Inhibited by Mx1 Protein Originated from Porcine

  2015influential citation