Degradation of  T Cell Receptor (TCR)–CD3-ζ Complexes after Antigenic Stimulation

T cell activation by specific antigen results in a rapid and long-lasting downregulation of triggered T cell receptors (TCRs). In this work, we investigated the fate of downregulated TCR– CD3-ζ complexes. T cells stimulated by peptide-pulsed antigen-presenting cells (APCs) undergo an antigen dose-dependent decrease of the total cellular content of TCR-β, CD3-ε, and ζ chains, as detected by FACS® analysis on fixed and permeabilized T–APC conjugates and by Western blot analysis on cell lysates. The time course of CD3-ζ chain consumption overlaps with that of TCR downregulation, indicating that internalized TCR–CD3 complexes are promptly degraded. Inhibitors of lysosomal function (bafilomycin A1, folimycin) markedly reduced ζ chain degradation, leading to the accumulation of ζ chain in large Lamp1+ vesicles. These results indicate that in T cell–APC conjugates, triggered TCRs are rapidly removed from the cell surface and are degraded in the lysosomal compartment.

• Publication year

  1997

• Venue

  Journal of Experimental Medicine

• Publication date

  1997-05-19

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.185.10.1859PMID 9151711PMCID 2196323
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Rapid turnover of the CD3ζ chain independent of the TCR-CD3 complex in normal T cells(正常T細胞におけるTCR-CD3複合体から独立したCD3ζ鎖の早い代謝回転)

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