L‐Glutamate Suppresses Amyloid β‐Protein‐Induced Stellation of Cultured Rat Cortical Astrocytes

Abstract: Alzheimer’s amyloid β‐protein (Aβ) has been reported to potentiate glutamate toxicity in neurons, but very little is known about interaction between Aβ and glutamate in astrocytes. Therefore, in the present study, we investigated the effects of Aβ and glutamate on morphology of astrocytes. Cultured rat cortical astrocytes exhibited polygonal morphology in the absence of stimulation and differentiated into process‐bearing stellate cells following exposure to Aβ (20 μM). L‐Glutamate (30‐1,000 μM) had no effect on astrocyte morphology in the absence of stimulation but strongly suppressed Aβ‐induced stellation. The suppressive effect of L‐glutamate on Aβ‐induced stellation was not mimicked by glutamate receptor agonists and not blocked by glutamate receptor antagonists. In contrast, the suppressive effect of L‐glutamate was mimicked by D‐ and L‐aspartate and transportable glutamate uptake inhibitors. These results suggest that Aβ‐induced astrocyte stellation is suppressed by a mechanism related to glutamate transporters.

• Publication year

  2000

• Venue

  Journal of Neurochemistry

• Publication date

  2000-01-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1046/j.1471-4159.2000.0740280.xPMID 10617130
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Amyloid-β peptide activates cultured astrocytes: morphological alterations, cytokine induction and nitric oxide release

  1998cited by this paper
• The NF-kappaB/Rel family of proteins mediates Abeta-induced neurotoxicity and glial activation.

  1998cited by this paper
• The NF-κB/Rel family of proteins mediates Aβ-induced neurotoxicity and glial activation

  1998cited by this paper
• Regulation of the glial Na+-dependent glutamate transporters by cyclic AMP analogs and neurons.

  1998cited by this paper
• Adenosine stimulates stellation of cultured rat cortical astrocytes.

  1998cited by this paper
• Amyloid-beta peptide activates cultured astrocytes: morphological alterations, cytokine induction and nitric oxide release.

  1998cited by this paper
• Neuronal Regulation of Glutamate Transporter Subtype Expression in Astrocytes

  1997cited by this paper
• Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance.

  1997cited by this paper
• Human amylin mimics amyloid beta protein-induced reactive gliosis and inhibition of cellular redox activity in cultured astrocytes.

  1997cited by this paper
• The inhibitory effects of beta-amyloid on glutamate and glucose uptakes by cultured astrocytes.

  1997cited by this paper
• Developmental changes in cyclic AMP-stimulated stellation of cultured rat cortical astrocytes.

  1997cited by this paper
• Nanomolar Amyloid β Protein‐Induced Inhibition of Cellular Redox Activity in Cultured Astrocytes

  1997cited by this paper
• Amyloid β Peptide (25–35) Inhibits Na+‐Dependent Glutamate Uptake in Rat Hippocampal Astrocyte Cultures

  1996cited by this paper
• beta-Amyloid-mediated inhibition of redox activity (MTT reduction) is not an indicator of astroglial degeneration.

  1996cited by this paper
• Menadione toxicity in cultured rat cortical astrocytes.

  1996cited by this paper
• An excitatory amino-acid transporter with properties of a ligand-gated chloride channel

  1995cited by this paper
• Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex

  1994cited by this paper
• Phorbol ester‐stimulated stellation in primary cultures of astrocytes from different brain regions

  1994cited by this paper
• Hydrogen peroxide mediates amyloid beta protein toxicity.

  1994cited by this paper
• Beta-amyloid-induced changes in cultured astrocytes parallel reactive astrocytosis associated with senile plaques in Alzheimer's disease.

  1994cited by this paper
• Intracellular pH changes produced by glutamate uptake in rat hippocampal slices.

  1994cited by this paper
• β-Amyloid-induced changes in cultured astrocytes parallel reactive astrocytosis associated with senile plaques in Alzheimer's disease

  1994cited by this paper
• Calcium-destabilizing and neurodegenerative effects of aggregated beta-amyloid peptide are attenuated by basic FGF.

  1993cited by this paper
• Expression cloning of a rat glutamate transporter.

  1993influential reference
• Glutamate transporters from brain

  1993cited by this paper
• Glutamate uptake from the synaptic cleft does not shape the decay of the non-NMDA component of the synaptic current.

  1993cited by this paper
• Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brain.

  1992cited by this paper
• Epidermal growth factor selectively enhances NMDA receptor-mediated increase of intracellular Ca2+ concentration in rat hippocampal neurons.

  1992cited by this paper
• beta-Amyloid peptides destabilize calcium homeostasis and render human cortical neurons vulnerable to excitotoxicity

  1992cited by this paper
• Cloning and expression of a rat brain L-glutamate transporter

  1992cited by this paper
• Primary structure and functional characterization of a high-affinity glutamate transporter

  1992cited by this paper
• In vitro aging of ß-amyloid protein causes peptide aggregation and neurotoxicity ☆

  1991cited by this paper
• Characterization of glutamate uptake systems in astrocyte primary cultures from rat brain

  1991cited by this paper
• In vitro aging of beta-amyloid protein causes peptide aggregation and neurotoxicity.

  1991cited by this paper
• Neurotrophic and neurotoxic effects of amyloid beta protein: reversal by tachykinin neuropeptides.

  1990cited by this paper
• β-Amyloid protein increases the vulnerability of cultured cortical neurons to excitotoxic damage

  1990cited by this paper
• Astrocytes and the plaques of Alzheimer's disease

  1990cited by this paper
• Potentiation by treatment with reserpine of alpha 2-adrenoceptor-mediated contractions of rat tail artery.

  1989cited by this paper
• Glutamate neurotoxicity and diseases of the nervous system.

  1988cited by this paper
• Alzheimer's disease. A double-labeling immunohistochemical study of senile plaques.

  1988cited by this paper
• Glutamate and the pathophysiology of hypoxic–ischemic brain damage

  1986cited by this paper
• Protein kinase C in astrocytes: a determinant of cell morphology.

  1986cited by this paper
• Excitotoxic models for neurodegenerative disorders.

  1984cited by this paper
• II: Excitotoxic models for neurodegenerative disorders

  1984cited by this paper
• Glial fibrillary acidic protein and Alzheimer‐type senile dementia

  1980cited by this paper
• Variability of the effects of serum-free medium, dibutyryl-cyclic AMP or theophylline on the morphology of cultured new-born rat astroblasts

  1975cited by this paper

• Transglutaminases Derived from Astrocytes Accelerate Amyloid β Aggregation

  2017cites this paper
• Adenosine A2A receptors modulate glutamate uptake in cultured astrocytes and gliosomes

  2012cites this paper
• Lactacystin Stimulates Stellation of Cultured Rat Cortical Astrocytes

  2009cites this paper
• Astrocyte and Neuron Intone Through Glutamate

  2008cites this paper
• Microglial cells in astroglial cultures: a cautionary note

  2007cites this paper
• l‐Glutamate activates RhoA GTPase leading to suppression of astrocyte stellation

  2006cites this paper
• Cholesterol deficiency perturbs actin signaling and glutamate homeostasis in hippocampal astrocytes.

  2006cites this paper
• Essential role for c‐jun N‐terminal kinase on tPA‐induced matrix metalloproteinase‐9 regulation in rat astrocytes

  2006cites this paper
• Positive allosteric modulators of the metabotropic glutamate receptor subtype 2 (mGluR2).

  2005cites this paper
• (2S,1'S,2'R,3'R)-2-(2'-Carboxy-3'-hydroxymethylcyclopropyl) glycine is a highly potent group 2 and 3 metabotropic glutamate receptor agonist with oral activity.

  2004cites this paper
• Structural association of glia with the various compartments of neurons

  2004cites this paper
• Essential role for ERK mitogen‐activated protein kinase in matrix metalloproteinase‐9 regulation in rat cortical astrocytes

  2003cites this paper
• Amyloid beta protein enhances the clearance of extracellular L-glutamate by cultured rat cortical astrocytes.

  2003cites this paper
• The extracellular signal-regulated kinase cascade suppresses amyloid beta protein-induced promotion of glutamate clearance in cultured rat cortical astrocytes.

  2003cites this paper
• Astrocyte stellation induced by Rho kinase inhibitors in culture.

  2003cites this paper
• (2S,1'S,2'S,3'R)-2-(2'-carboxy-3'-methylcyclopropyl) glycine is a potent and selective metabotropic group 2 receptor agonist with anxiolytic properties.

  2002cites this paper
• Human glioma cells and undifferentiated primary astrocytes that express aberrant EAAT2 mRNA inhibit normal EAAT2 protein expression and prevent cell death.

  2002cites this paper
• L-glutamate suppresses astrocyte stellation induced by actin breakdown in culture.

  2001cites this paper
• Possible linkage between glutamate transporter and mitogen‐activated protein kinase cascade in cultured rat cortical astrocytes

  2001cites this paper
• Na+ and K+ dependence of morphological changes of cultured rat cortical astrocytes.

  2001cites this paper
• Na+ and K+ dependence of L-glutamate-induced suppression of astrocyte stellation in culture.

  2001cites this paper
• Amyloid β neurotoxicity not mediated by the mitogen-activated protein kinase cascade in cultured rat hippocampal and cortical neurons

  2000cites this paper
• cAMP-induced stellation in primary astrocyte cultures with regional heterogeneity.

  2000cites this paper