Effects of procaine amide on the electrophysiologic properties of the canine ventricular conducting system.

Microelectrode techniques were used to study the effects of procaine amide (PA), 10 to 90 µg/ml, on electrophysiologic properties of canine Purkinje fibers (PF) and ventricular muscle perfused with Tyrode's solution containing 2.5, 4.0 or 5.5 mM KCl. Automaticity of spontaneously beating PF decreased within 40 minutes of perfusion with [PF] = 10 µg/ml; this was the only consistent electrophysiologic change at [PA]<30 µg/ml. After 30 minutes of perfusion with [PA] = 30 µg/ml, action potential amplitude and maximal slope of phase 0 decreased, and conduction throughout the ventricular conducting system was slowed, especially that in ventricular muscle. The voltage-time course of repolarization was altered in such a way that action potentials dissimilar in duration, recorded from sites proximal to, distal to and at the area of maximum action potential duration became similar. Simultaneously action potential duration measured to full repolarization and effective refractory periods were prolonged. Changes were most prominent at [K+] = 2.5 and least apparent at [K+] = 5.5 mM. At [PA] = 90 µg/ml the aforementioned changes were accentuated.

• Publication year

  1973

• Venue

  Journal of Pharmacology and Experimental Therapeutics

• Publication date

  1973-06-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0022-3565(25)29526-xPMID 4712643
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The electrophysiologic effects of procaine amide were most prominent in Tyrode's solution with 2.5 mM KCl, least apparent at 5.5 mM KCl, and accentuated at 90 µg/ml.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review
• At 30 µg/ml procaine amide, action potential amplitude and maximal slope of phase 0 decreased, and effective refractory period was prolonged.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review
• Automaticity of spontaneously beating canine Purkinje fibers decreased within 40 minutes after perfusion with 10 µg/ml procaine amide, and this was the only consistent electrophysiologic change below 30 µg/ml.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review

• action potential amplitude

  electrophysiologic measure

  The peak voltage height of a recorded action potential.

  Aliases: APA

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review
• automaticity

  electrophysiologic property

  The spontaneous pacemaker activity of the Purkinje fibers.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review
• canine purkinje fibers

  cardiac tissue, conducting fiber

  Specialized cardiac conducting fibers from dog ventricles that were recorded with microelectrodes.

  Aliases: PF

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review
• effective refractory period

  electrophysiologic property

  The post-activation interval during which the tissue cannot be re-excited.

  Aliases: ERP

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review
• maximal slope of phase 0

  electrophysiologic measure

  The steepest upstroke rate during phase 0 depolarization of the action potential.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review
• procaine amide

  drug

  An antiarrhythmic agent perfused at 10 to 90 µg/ml in the experiments.

  Aliases: PA

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review
• tyrode's solution with kcl

  solution, perfusate

  The perfusion medium whose potassium chloride concentration was set to 2.5, 4.0, or 5.5 mM.

  Aliases: Tyrode's solution

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review
• ventricular muscle

  cardiac tissue

  Contractile ventricular myocardium examined alongside the conducting system.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewimjlk (vdp8mqzes2) reviewAK (4715169a40) review

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