Alveolar macrophages regulate neutrophil recruitment in endotoxin-induced lung injury

BackgroundAlveolar macrophages play an important role during the development of acute inflammatory lung injury. In the present study, in vivo alveolar macrophage depletion was performed by intratracheal application of dichloromethylene diphosphonate-liposomes in order to study the role of these effector cells in the early endotoxin-induced lung injury.MethodsLipopolysaccharide was applied intratracheally and the inflammatory reaction was assessed 4 hours later. Neutrophil accumulation and expression of inflammatory mediators were determined. To further analyze in vivo observations, in vitro experiments with alveolar epithelial cells and alveolar macrophages were performed.ResultsA 320% increase of polymorphonuclear leukocytes in bronchoalveolar lavage fluid was observed in macrophage-depleted compared to macrophage-competent lipopolysaccharide-animals. This neutrophil recruitment was also confirmed in the interstitial space. Monocyte chemoattractant protein-1 concentration in bronchoalveolar lavage fluid was significantly increased in the absence of alveolar macrophages. This phenomenon was underlined by in vitro experiments with alveolar epithelial cells and alveolar macrophages. Neutralizing monocyte chemoattractant protein-1 in the airways diminished neutrophil accumulation.ConclusionThese data suggest that alveolar macorphages play an important role in early endotoxin-induced lung injury. They prevent neutrophil influx by controlling monocyte chemoattractant protein-1 production through alveolar epithelial cells. Alveolar macrophages might therefore possess robust anti-inflammatory effects.

• Publication year

  2005

• Venue

  Respiratory Research

• Publication date

  2005-06-22

• Fields of study

  Medicine

• Identifiers
  DOI 10.1186/1465-9921-6-61PMID 15972102PMCID 1188075
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The effects and comparative differences of neutrophil specific chemokines on neutrophil chemotaxis of the neonate.

  2005cited by this paper
• Monocyte chemotactic protein-1 regulates leukocyte recruitment during gastric ulcer recurrence induced by tumor necrosis factor-alpha.

  2004cited by this paper
• Antimacrophage chemokine treatment prevents neutrophil and macrophage influx in hyperoxia-exposed newborn rat lung.

  2004cited by this paper
• Decreased alveolar oxygen induces lung inflammation.

  2003cited by this paper
• Macrophages exposed to Mycobacterium tuberculosis release chemokines able to recruit selected leucocyte subpopulations: focus on gammadelta cells.

  2003cited by this paper
• Macrophages exposed to Mycobacterium tuberculosis release chemokines able to recruit selected leucocyte subpopulations: focus on γδ cells

  2003cited by this paper
• Macrophages Are Necessary for Maximal Nuclear Factor- κ B Activation in Response to Endotoxin

  2002cited by this paper
• Role of alveolar epithelial ICAM-1 in lipopolysaccharide-induced lung inflammation

  2002cited by this paper
• Role of resident alveolar macrophages in leukocyte traffic into the alveolar air space of intact mice.

  2002cited by this paper
• Macrophages are necessary for maximal nuclear factor-kappa B activation in response to endotoxin.

  2002cited by this paper
• Interaction of alveolar macrophages and airway epithelial cells following exposure to particulate matter produces mediators that stimulate the bone marrow.

  2002cited by this paper
• Alveolar JE/MCP-1 and endotoxin synergize to provoke lung cytokine upregulation, sequential neutrophil and monocyte influx, and vascular leakage in mice.

  2001cited by this paper
• Roles of Macrophages in Measles Virus Infection of Genetically Modified Mice

  2001cited by this paper
• Hypoxia mediates increased neutrophil and macrophage adhesiveness to alveolar epithelial cells.

  2001influential reference
• Lipopolysaccharide induces functional ICAM-1 expression in rat alveolar epithelial cells in vitro.

  2000cited by this paper
• Pulmonary immunity to Listeria is enhanced by elimination of alveolar macrophages.

  1999cited by this paper
• Essential role of alveolar macrophages in intrapulmonary activation of NF-kappaB.

  1999cited by this paper
• Essential Role of Alveolar Macrophages in Intrapulmonary Activation of NF- κ B

  1999cited by this paper
• Chronic inflammation upregulates chemokine receptors and induces neutrophil migration to monocyte chemoattractant protein-1.

  1999cited by this paper
• Hyaluronan induces monocyte chemoattractant protein-1 expression in renal tubular epithelial cells.

  1998cited by this paper
• Lung inflammatory response in pneumonia.

  1998influential reference
• Adhesion Molecules in Inflammatory Diseases

  1998cited by this paper
• Lipopolysaccharide-induced alveolar epithelial permeability: the role of nitric oxide.

  1998cited by this paper
• Alveolar macrophages are required for protective pulmonary defenses in murine Klebsiella pneumonia: elimination of alveolar macrophages increases neutrophil recruitment but decreases bacterial clearance and survival

  1997cited by this paper
• Expression of lung vascular and airway ICAM-1 after exposure to bacterial lipopolysaccharide.

  1997cited by this paper
• Depletion of alveolar macrophages decreases neutrophil chemotaxis to Pseudomonas airspace infections.

  1996cited by this paper
• Modulation of cell membrane potential in cultured vascular endothelium.

  1996cited by this paper
• Characterization of murine lung interstitial macrophages in comparison with alveolar macrophages in vitro.

  1996cited by this paper
• Response of alveolar macrophage-depleted rats to hyperoxia.

  1995cited by this paper
• Intratracheal administration of endotoxin and cytokines. VI. Antiserum to CINC inhibits acute inflammation.

  1995cited by this paper
• Cytokine expression by neutrophils and macrophages in vivo: endotoxin induces tumor necrosis factor-alpha, macrophage inflammatory protein-2, interleukin-1 beta, and interleukin-6 but not RANTES or transforming growth factor-beta 1 mRNA expression in acute lung inflammation.

  1994cited by this paper
• Intratracheal administration of endotoxin and cytokines. VII. The soluble interleukin-1 receptor and the soluble tumor necrosis factor receptor II (p80) inhibit acute inflammation.

  1994cited by this paper
• Contrasting effects of alveolar macrophages and neutrophils on asbestos-induced pulmonary epithelial cell injury.

  1994cited by this paper
• Polymorphonuclear leukocytes as a significant source of tumor necrosis factor-alpha in endotoxin-challenged lung tissue.

  1993cited by this paper
• MCP-1 expression by rat type II alveolar epithelial cells in primary culture.

  1993cited by this paper
• Macrophages and polymorphonuclear neutrophils in lung defense and injury.

  1990cited by this paper
• Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice

  1989cited by this paper
• The alveolar macrophage.

  1986cited by this paper
• Lung myeloperoxidase as a measure of pulmonary leukostasis in rabbits.

  1985cited by this paper
• The alveolar macrophage.

  1984cited by this paper

• The Involvement of MicroRNAs in Innate Immunity and Cystic Fibrosis Lung Disease: A Narrative Review

  2026cites this paper
• Macrophage TRIM21 lactylation exacerbates infection-induced orchitis through enhancing STAT1-mediated CXCL9 and CXCL10 production

  2026cites this paper
• Caspase‐1 Dependent Neutrophil Pyroptosis Contributes to Fine Particulate Matter‐Induced Lung Inflammation

  2025cites this paper
• Lyophilized MSC-EVs attenuates COVID-19 pathogenesis by regulating the JAK/STAT pathway

  2025cites this paper
• Proteome profiling of the lung tissues of the mice co-infected with H9N2 influenza A virus and Pseudomonas aeruginosa.

  2025cites this paper
• A vasculature-resident innate lymphoid cell population in mouse lungs

  2025cites this paper
• Cirsium japonicum leaf extract attenuated lipopolysaccharide-induced acute respiratory distress syndrome in mice via suppression of the NLRP3 and HIF1α pathways.

  2025cites this paper
• Inhibition of peptidyl arginine deiminase-4 ameliorated pulmonary fibrosis via modulating M1/M2 polarisation of macrophages.

  2025cites this paper
• Liposomal 188Rhenium Plus Macrophage Depletion Enhances Anti‐PD‐L1 Efficacy and B Cell Infiltration Against Lung Metastatic Cancer

  2025cites this paper
• Lung Immune Cell Niches and the Discovery of New Cell Subtypes

  2024cites this paper
• Chronic TNF exposure induces glucocorticoid‐like immunosuppression in the alveolar macrophages of aged mice that enhances their susceptibility to pneumonia

  2024cites this paper
• Transplantation of Alveolar Macrophages Improves the Efficacy of Endothelial Progenitor Cell Therapy in Mouse Model of Bronchopulmonary Dysplasia.

  2024cites this paper
• iRhom2 deficiency reduces sepsis-induced mortality associated with the attenuation of lung macrophages in mice

  2024cites this paper
• Ism1 deficiency in mice exacerbates bleomycin-induced pulmonary fibrosis with enhanced cellular senescence and delayed fibrosis resolution

  2024cites this paper
• Peripheral blood monocyte count and outcomes in patients with interstitial lung disease: a systematic review and meta-analysis

  2023cites this paper
• Immunomodulatory Effects of Subacute Inhalation Exposure to Copper Oxide Nanoparticles in House Dust Mite-Induced Asthma

  2023cites this paper
• Stimulation of lipopolysaccharide from Pseudomonas aeruginosa following H9N2 IAV infection exacerbates inflammatory responses of alveolar macrophages and decreases virus replication.

  2023cites this paper
• Myeloid Cell Derived IL1β Contributes to Pulmonary Vascular Remodeling in Heart Failure with Preserved Ejection Fraction

  2023cites this paper
• MiR-223-3p attenuates radiation-induced inflammatory response and inhibits the activation of NLRP3 inflammasome in macrophages.

  2023cites this paper
• Myeloid Cell Derived IL1β Contributes to Pulmonary Hypertension in HFpEF

  2023cites this paper
• Techniques for navigating postsurgical adhesions: Insights into mechanisms and future directions

  2023cites this paper
• Identification and immunological characterization of endoplasmic reticulum stress-related molecular subtypes in bronchopulmonary dysplasia based on machine learning

  2023cites this paper
• Diesel vehicles-derived PM2.5 induces lung and cardiovascular injury attenuates by Securiniga suffruticosa: Involvement of NF-κB-mediated NLRP3 inflammasome activation pathway.

  2023cites this paper
• S1P lyase inhibition prevents lung injury following high pressure-controlled mechanical ventilation in aging mice.

  2022cites this paper
• Dose–response relationship of pulmonary disorders by inhalation exposure to cross-linked water-soluble acrylic acid polymers in F344 rats

  2022cites this paper
• Senescent macrophages alter fibroblast fibrogenesis in response to SARS-CoV-2 infection

  2022cites this paper
• Nicotinic acetylcholine receptor agonist reduces acute lung injury after renal ischemia-reperfusion injury by acting on splenic macrophages in mice.

  2022cites this paper
• HIF-1α induces glycolytic reprograming in tissue-resident alveolar macrophages to promote cell survival during acute lung injury

  2022cites this paper
• Inflammation driven metabolic regulation and adaptation in macrophages.

  2022cites this paper
• Pathogenesis of COPD at the cellular and molecular level.

  2022cites this paper
• Multiple Death Pathways of Neutrophils Regulate Alveolar Macrophage Proliferation

  2022cites this paper
• Alveolar macrophages protect mice from MERS-CoV-induced pneumonia and severe disease

  2022cites this paper
• Neutrophils in aging and aging‐related pathologies

  2022cites this paper
• Galectin-3 inhibitor GB0139 protects against acute lung injury by inhibiting neutrophil recruitment and activation

  2022cites this paper
• Association of immune cell recruitment and BPD development

  2022cites this paper
• Toll-Like Receptor 2 Modulates Pulmonary Inflammation and TNF-α Release Mediated by Mycoplasma pneumoniae

  2022cites this paper
• Absence of CCR2 Promotes Proliferation of Alveolar Macrophages That Control Lung Inflammation in Acute Respiratory Distress Syndrome in Mice

  2022cites this paper
• Qu-Yu-Jie-Du Decoction Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulation of Neutrophils and Macrophage Infiltration

  2022cites this paper
• Selenium-Containing Compound Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Regulating the MAPK/AP-1 Pathway

  2021cites this paper
• Diversity of Macrophages in Lung Homeostasis and Diseases

  2021cites this paper
• The Role of Macrophages During Mammalian Tissue Remodeling and Regeneration Under Infectious and Non-Infectious Conditions

  2021cites this paper
• Geranylgeranyl diphosphate synthase deficiency hyperactivates macrophages and aggravates lipopolysaccharide-induced acute lung injury.

  2021cites this paper
• The Role of Macrophages in the Development of Acute and Chronic Inflammatory Lung Diseases

  2021cites this paper
• Investigation of Calprotectin Positive Leukocytes in Canine Soft Tissue Tumors

  2021cites this paper
• lincRNA-Cox2 Functions to Regulate Inflammation in Alveolar Macrophages during Acute Lung Injury

  2021cites this paper
• Selenium-containing compound ameliorates lipopolysaccharide-induced acute lung injury via regulating MAPKs/AP-1 pathway

  2021cites this paper
• Corylin Ameliorates LPS-Induced Acute Lung Injury via Suppressing the MAPKs and IL-6/STAT3 Signaling Pathways

  2021cites this paper
• A review of physiological and cellular mechanisms underlying fibrotic postoperative adhesion

  2021cites this paper
• The Role of Macrophages in the Pathogenesis of SARS-CoV-2-Associated Acute Respiratory Distress Syndrome

  2021cites this paper
• Diesel exhaust particulates induce neutrophilic lung in�ammation by modulating endoplasmic reticulum stress-mediated CXCL1/KC expression in alveolar macrophages

  2021cites this paper
• Correction to: Dose–response relationship of pulmonary disorders by inhalation exposure to cross-linked water-soluble acrylic acid polymers in F344 rats

  2021cites this paper
• A review of Sulfur Mustard-induced pulmonary immunopathology: An Alveolar Macrophage Approach.

  2020cites this paper
• Tissue-Resident Macrophages in the Control of Infection and Resolution of Inflammation.

  2020cites this paper
• Patrolling Alveolar Macrophages Conceal Bacteria from the Immune System to Maintain Homeostasis.

  2020cites this paper
• Diesel Exhaust Particulates Induce Neutrophilic Lung Inflammation by Modulating Endoplasmic Reticulum Stress-Mediated CXCL1/KC Expression in Alveolar Macrophages

  2020cites this paper
• An aberrant STAT pathway is central to COVID-19

  2020cites this paper
• Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway.

  2020cites this paper
• The CCL2/CCR2 axis is critical to recruiting macrophages into acellular nerve allograft bridging a nerve gap to promote angiogenesis and regeneration.

  2020cites this paper
• Lung Toxicity Analysis of Nano-Sized Kaolin and Bentonite: Missing Indications for a Common Grouping

  2020cites this paper
• Mitochondria-Modulating Porous Se@SiO2 Nanoparticles Provide Resistance to Oxidative Injury in Airway Epithelial Cells: Implications for Acute Lung Injury

  2020cites this paper
• The Diverse Roles of Phagocytes During Bacterial and Fungal Infections and Sterile Inflammation: Lessons From Zebrafish

  2020cites this paper
• THE IMPACT OF AGING AND MECHANICAL INJURY ON ALVEOLAR EPITHELIAL AND MACROPHAGE RESPONSES IN ACUTE LUNG INJURY AND INFLAMMATION

  2020cites this paper
• Residual endotoxin induces primary graft dysfunction through ischemia/reperfusion-primed alveolar macrophages.

  2020cites this paper
• Harnessing the chronic inflammatory environment in non-small cell lung cancer for biomarker discovery

  2019cites this paper
• Network Pharmacology-Based Investigation of Protective Mechanism of Aster tataricus on Lipopolysaccharide-Induced Acute Lung Injury

  2019cites this paper
• Uncovering the Mechanisms of Staphylococcus aureus Enterotoxin A-induced Systemic and Pulmonary Inflammation

  2019cites this paper
• Non-small cell lung cancer tumour antigen, MUC-1 peptide-loaded non-aggregated poly (lactide-co-glycolide) nanoparticles augmented cellular uptake in mouse professional antigen-presenting cells: optimisation and characterisation

  2019cites this paper
• PIM1 inhibitor SMI-4a attenuated lipopolysaccharide-induced acute lung injury through suppressing macrophage inflammatory responses via modulating p65 phosphorylation.

  2019cites this paper
• Coordinated Induction of Antimicrobial Response Factors in Systemic Lupus Erythematosus

  2019cites this paper
• CXCR4 knockdown prevents inflammatory cytokine expression in macrophages by suppressing activation of MAPK and NF-κB signaling pathways

  2019cites this paper
• Human Pleural Fluid Elicits Pyruvate and Phenylalanine Metabolism in Acinetobacter baumannii to Enhance Cytotoxicity and Immune Evasion

  2019cites this paper
• Transcriptomic and epigenetic mechanisms underlying myeloid diversity in the lung

  2019cites this paper
• Simvastatin Nanoparticles Reduce Inflammation in LPS-Stimulated Alveolar Macrophages.

  2019cites this paper
• Exosomes in postshock mesenteric lymph are key mediators of acute lung injury triggering the macrophage activation via Toll‐like receptor 4

  2018cites this paper
• Monocyte-derived Alveolar Macrophages: The Dark Side of Lung Repair?

  2018cites this paper
• Detrimental Influence of Alveolar Macrophages on Protective Humoral Immunity during Francisella tularensis SchuS4 Pulmonary Infection

  2018cites this paper
• Substrate accumulation and extracellular matrix remodelling promote persistent upper airway disease in mucopolysaccharidosis patients on enzyme replacement therapy

  2018cites this paper
• Saquinavir plus methylprednisolone ameliorates experimental acute lung injury

  2018cites this paper
• The Role of Macrophages in the Pathogenesis of ALI/ARDS

  2018cites this paper
• Circadian clock component REV-ERBα controls homeostatic regulation of pulmonary inflammation

  2018cites this paper
• Effects of Dexmedetomidine Infusion on Inflammatory Responses and Injury of Lung Tidal Volume Changes during One-Lung Ventilation in Thoracoscopic Surgery: A Randomized Controlled Trial

  2018cites this paper
• Partners in crime: neutrophils and monocytes/macrophages in inflammation and disease

  2018cites this paper
• Comment on “Serum human epididymis protein 4 is associated with the treatment response of concurrent chemo-radiotherapy and prognosis in patients with locally advanced non-small cell lung cancer” by Lan WG et al.

  2018cites this paper
• Dysregulated Functions of Lung Macrophage Populations in COPD

  2018cites this paper
• Evaluating the Impact of Novel Bone Marrow Cell-secreted Factors, C19orf10 and C1orf54, on Immunomodulation, Lung Cell Proliferation, and Resistance to Injury

  2017cites this paper
• CD169+ Macrophages Restrain Systemic Inflammation Induced by Staphylococcus aureus Enterotoxin A Lung Response

  2017cites this paper
• Depletion of circulating monocytes suppresses IL-17 and HMGB1 expression in mice with LPS-induced acute lung injury.

  2017influential citation
• Translational Research in Acute Lung Injury and Pulmonary Fibrosis Depletion of circulating monocytes suppresses IL-17 and HMGB 1 expression in mice with LPS-induced acute lung injury

  2017influential citation
• Innate Immunity at Birth

  2017cites this paper
• The blood‐borne sialyltransferase ST6Gal‐1 is a negative systemic regulator of granulopoiesis

  2017cites this paper
• Role of REV-ERBα in the regulation of lung inflammation

  2017cites this paper
• Zerumbone reduced the inflammatory response of acute lung injury in endotoxin-treated mice via Akt-NFκB pathway.

  2017cites this paper
• LRP1: A chameleon receptor of lung inflammation and repair.

  2017cites this paper
• The regulation of acute immune responses to the bacterial lung pathogen Legionella pneumophila

  2017cites this paper
• Necrostatin-1 attenuates lipopolysaccharide-induced acute lung injury in mice

  2017cites this paper
• The protective effect and mechanism of sevoflurane on LPS-induced acute lung injury in mice.

  2017cites this paper
• Nogo-B Facilitates LPS-Mediated Immune Responses by Up-Regulation of TLR4-Signaling in Macrophage RAW264.7

  2017cites this paper
• XB130 deficiency enhances lipopolysaccharide-induced septic response and acute lung injury

  2016cites this paper
• Suppressive oligonucleotides inhibit inflammation in a murine model of mechanical ventilator induced lung injury.

  2016cites this paper
• Activated prostaglandin D2 receptors on macrophages enhance neutrophil recruitment into the lung

  2016cites this paper