Crystal structure of the cytochrome P-450CAM active site mutant Thr252Ala.

No abstract is available for this paper.

• Publication year

  1991

• Venue

  Biochemistry

• Publication date

  1991-12-03

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI00112A008PMID 1742281
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Crystal structures of metyrapone- and phenylimidazole-inhibited complexes of cytochrome P-450cam

  1993cited by this paper
• Reactions and significance of cytochrome P-450 enzymes.

  1991cited by this paper
• The structural basis for substrate-induced changes in redox potential and spin equilibrium in cytochrome P-450CAM.

  1991cited by this paper
• Crystal structures of cytochrome P-450CAM complexed with camphane, thiocamphor, and adamantane: factors controlling P-450 substrate hydroxylation.

  1991cited by this paper
• Solvation of the active site of cytochrome P450-cam

  1990cited by this paper
• Formation, crystal structure, and rearrangement of a cytochrome P-450cam iron-phenyl complex.

  1990cited by this paper
• Uncoupling of the cytochrome P-450cam monooxygenase reaction by a single mutation, threonine-252 to alanine or valine: possible role of the hydroxy amino acid in oxygen activation.

  1989cited by this paper
• Point mutations at threonine-301 modify substrate specificity of rabbit liver microsomal cytochromes P-450 (laurate (omega-1)-hydroxylase and testosterone 16 alpha-hydroxylase).

  1989cited by this paper
• Crystal structure of the carbon monoxide-substrate-cytochrome P-450CAM ternary complex.

  1989cited by this paper
• Site-directed mutageneses of rat liver cytochrome P-450d: catalytic activities toward benzphetamine and 7-ethoxycoumarin.

  1989cited by this paper
• Mutations at the distal and proximal sites of cytochrome P-450d changed regio-specificity of acetanilide hydroxylations.

  1989cited by this paper
• Secondary structure prediction of 52 membrane-bound cytochromes P450 shows a strong structural similarity to P450cam.

  1989cited by this paper
• Point mutations at threonine-301 modify substrate specificity of rabbit liver microsomal cytochromes P-450 (laurate (ω-1)-hydroxylase and testosterone 16α-hydroxylase)

  1989cited by this paper
• Molecular recognition in cytochrome P-450: Alteration of regioselective alkane hydroxylation via protein engineering

  1989cited by this paper
• A conserved residue of cytochrome P-450 is involved in heme-oxygen stability and activation

  1989cited by this paper
• The roles of active site hydrogen bonding in cytochrome P-450cam as revealed by site-directed mutagenesis.

  1988cited by this paper
• Deuterium isotope effects in norcamphor metabolism by cytochrome P-450cam: kinetic evidence for the two-electron reduction of a high-valent iron-oxo intermediate.

  1988cited by this paper
• On the membrane topology of vertebrate cytochrome P-450 proteins.

  1988cited by this paper
• High-resolution crystal structure of cytochrome P450cam.

  1987cited by this paper
• P450 genes: structure, evolution, and regulation.

  1987cited by this paper
• Crystal structures of metyrapone- and phenylimidazole-inhibited complexes of cytochrome P-450cam.

  1987cited by this paper
• Metabolic switching in cytochrome P-450cam: Deuterium isotope effects on regiospecificity and the monooxygenase/oxidase ratio

  1987cited by this paper
• Nucleotide sequence of the Pseudomonas putida cytochrome P-450cam gene and its expression in Escherichia coli.

  1986cited by this paper
• Crystal structure of substrate-free Pseudomonas putida cytochrome P-450.

  1986cited by this paper
• Control of heme protein redox potential and reduction rate: linear free energy relation between potential and ferric spin state equilibrium

  1985cited by this paper
• The 2.6-A crystal structure of Pseudomonas putida cytochrome P-450.

  1985cited by this paper
• Regioselectivity in the cytochromes P-450: control by protein constraints and by chemical reactivities.

  1984cited by this paper
• On the stoichiometry of the oxidase and monooxygenase reactions catalyzed by liver microsomal cytochrome P-450. Products of oxygen reduction.

  1984cited by this paper
• Complete stoichiometry of free NADPH oxidation in liver microsomes.

  1982cited by this paper
• Preliminary crystallographic data on cytochrome P-450CAM.

  1982cited by this paper
• Genetic mechanisms controlling the induction of polysubstrate monooxygenase (P-450) activities.

  1981cited by this paper
• Cytochrome P450 and biological hydroxylation reactions.

  1979cited by this paper
• A graphics model building and refinement system for macromolecules

  1978cited by this paper
• The Protein Data Bank: a computer-based archival file for macromolecular structures.

  1977cited by this paper
• The role of NADH in uncoupled microsomal monoxygenations.

  1974cited by this paper
• Solvent effects on reversible formation and oxidative stability of heme-oxygen complexes.

  1974cited by this paper

• Structural insights into S-lignin O-demethylation via a rare class of heme peroxygenase enzymes

  2025cites this paper
• Identification and Reactivity of Peroxyhemiacetal Intermediate during Aldehyde Deformylation by a Heme Ferric-Peroxide Complex.

  2025cites this paper
• A Thermostable Heme Protein Fold Adapted for Stereoselective C−H Bond Hydroxylation Using Peroxygenase Activity

  2024cites this paper
• The S-lignin O-demethylase SyoA: Structural insights into a new class of heme peroxygenase enzymes

  2024cites this paper
• Selective carbon-hydrogen bond hydroxylation using an engineered cytochrome P450 peroxygenase.

  2023cites this paper
• Characterisation of the heme aqua-ligand coordination environment in an engineered peroxygenase cytochrome P450 variant.

  2023cites this paper
• Computationally guided bioengineering of the active site, substrate access pathway, and water channels of thermostable cytochrome P450, CYP175A1, for catalyzing the alkane hydroxylation reaction

  2023cites this paper
• Selective Oxidations Using a Cytochrome P450 Enzyme Variant Driven with Surrogate Oxygen Donors and Light

  2022cites this paper
• An Altered Heme Environment in an Engineered Cytochrome P450 Enzyme Enables the Switch from Monooxygenase to Peroxygenase Activity

  2022cites this paper
• The 16α‐Hydroxylation of Progesterone by Cytochrome P450 107X1 from Streptomyces avermitilis

  2022influential citation
• Depicting the proton relay network in human aromatase: New insights into the role of the alcohol‐acid pair

  2022influential citation
• Understanding the Mechanistic Requirements for Efficient and Stereoselective Alkene Epoxidation by a Cytochrome P450 Enzyme

  2021cites this paper
• Emerging Structural and Functional Diversity in Proteins With Dioxygen-Reactive Dinuclear Transition Metal Cofactors

  2020cites this paper
• Mechanism-Guided Design and Discovery of Efficient P450-Derived C-H Amination Biocatalysts.

  2020cites this paper
• Structural insights into the role of the acid-alcohol pair of residues required for dioxygen activation in cytochrome P450 enzymes

  2020cites this paper
• Nature's Machinery, Repurposed: Expanding the Repertoire of Iron-Dependent Oxygenases.

  2020cites this paper
• A heterogeneous bio-inspired peroxide shunt for catalytic oxidation of organic molecules.

  2020cites this paper
• Proton relay network in P450cam formed upon docking of putidaredoxin

  2020cites this paper
• A BIOPHYSICAL INVESTIGATION OF STABILITY, LIGAND BINDING, AND IRON STATE OF CYP102A1

  2020cites this paper
• Proton Relay Network in P450cam is Formed Upon Docking of its Redox Partner Putidaredoxin (Pdx)

  2019cites this paper
• Effects of polymorphic variation on the thermostability of heterogenous populations of CYP3A4 and CYP2C9 enzymes in solution

  2018cites this paper
• Binding of Distinct Substrate Conformations Enables Hydroxylation of Remote Sites in Thaxtomin D by Cytochrome P450 TxtC.

  2018cites this paper
• Structure of cytochrome P450 2B4 with an acetate ligand and an active site hydrogen bond network similar to oxyferrous P450cam.

  2018cites this paper
• Harnessing P450 enzymes as biocatalysts for selective C-H bond hydroxylation

  2018cites this paper
• Heme-utilizing ribozymes and DNAzymes: Biological impacts, structural aspects, and a kinetic model of activation

  2017cites this paper
• Catalytic Determinants of Alkene Production by the Cytochrome P450 Peroxygenase OleTJE

  2017cites this paper
• Characterisation and engineering of alkene producing P450 peroxygenases for bioenergy applications

  2017cites this paper
• Investigating the roles of T224 and T232 in the oxidation of cinnamaldehyde catalyzed by myxobacterial CYP260B1

  2017cites this paper
• Utilising CYP199A4 from Rhodopseudomonas palustris HaA2 for biocatalysis and mechanistic studies

  2016cites this paper
• Structural characterization of CYP260A1 from Sorangium cellulosum to investigate the 1α‐hydroxylation of a mineralocorticoid

  2016cites this paper
• Interactions of Cytochrome P450 3A4 with Phospholipid Bilayer Nanodiscs

  2016cites this paper
• Etude et applications de nouveaux modèles géométriques des canaux d'accès au site actif de certains cytochromes P450 humains par des ligands volumineux

  2016cites this paper
• In vivo and in vitro hydroxylation of cineole and camphor by cytochromes P450CYP101A1, CYP101B1 and N242A CYP176A1

  2016cites this paper
• Valence tautomerism in synthetic models of cytochrome P450

  2016cites this paper
• The role of cytochrome P450 BM3 phenylalanine-87 and threonine-268 in binding organic hydroperoxides.

  2016cites this paper
• Identification of new substrates for the CYP106A1‐mediated 11‐oxidation and investigation of the reaction mechanism

  2015cites this paper
• Biological diversity of cytochrome P450 redox partner systems.

  2015cites this paper
• Oxidizing intermediates in P450 catalysis: a case for multiple oxidants.

  2015cites this paper
• Allelic variation and multigenic metabolic activity of cytochrome P450s confer insecticide resistance in field populations of anopheles funestus s.s., a major malaria vector in Africa

  2015cites this paper
• Monooxygenase, peroxidase and peroxygenase properties and reaction mechanisms of cytochrome P450 enzymes.

  2015cites this paper
• Spectroscopic and Kinetic Evidence for the Crucial Role of Compound 0 in the P450cam -Catalyzed Hydroxylation of Camphor by Hydrogen Peroxide.

  2015cites this paper
• Activation of Molecular Oxygen in Cytochromes P450

  2015cites this paper
• Steroid conversions with the CYP106A subfamily from Bacillus megaterium

  2015cites this paper
• Microbial Cytochromes P450

  2015cites this paper
• QM/MM Studies of Structure and Reactivity of Cytochrome P450 Enzymes: Methodology and Selected Applications

  2014cites this paper
• Heme enzyme structure and function.

  2014cites this paper
• Fifty Years of Cytochrome P450 Research: Examples of What We Know and Do Not Know

  2014cites this paper
• Structure and Biochemical Properties of the Alkene Producing Cytochrome P450 OleTJE (CYP152L1) from the Jeotgalicoccus sp. 8456 Bacterium*

  2014influential citation
• Kinetic solvent isotope effect in steady‐state turnover by CYP19A1 suggests involvement of Compound 1 for both hydroxylation and aromatization steps

  2014cites this paper
• Active site proton delivery and the lyase activity of human CYP17A1.

  2014cites this paper
• Water in Photosystem II: structural, functional and mechanistic considerations.

  2014cites this paper
• Selective steroid hydroxylation by bacterial cytochrome P450 monooxygenases

  2014cites this paper
• Combining in silico protein stability calculations with structure-function relationships to explore the effect of polymorphic variation on cytochrome P450 drug metabolism.

  2013cites this paper
• Unusual properties of the cytochrome P450 superfamily

  2013cites this paper
• Role of the highly conserved threonine in cytochrome P450 2E1: prevention of H2O2-induced inactivation during electron transfer.

  2013cites this paper
• Crystal structures and functional characterization of wild-type CYP101D1 and its active site mutants.

  2013cites this paper
• Overview on theoretical studies discriminating the two-oxidant versus two-state-reactivity models for substrate monoxygenation by cytochrome P450 enzymes.

  2013cites this paper
• P450cin active site water: implications for substrate binding and solvent accessibility.

  2013cites this paper
• Biochemical Processes and Metal Complexes’ Role as Catalysts

  2012cites this paper
• Novel CYP11B2 mutation causing aldosterone synthase (P450c11AS) deficiency

  2012cites this paper
• Changing the Regioselectivity of a P450 from C15 to C11 Hydroxylation of Progesterone

  2012cites this paper
• Unusual Spectroscopic and Ligand Binding Properties of the Cytochrome P450-Flavodoxin Fusion Enzyme XplA*

  2012cites this paper
• Thr302 Is the Site for the Covalent Modification of Human Cytochrome P450 2B6 Leading to Mechanism-Based Inactivation by tert-Butylphenylacetylene

  2011cites this paper
• Insights into the different dioxygen activation pathways of methane and toluene monooxygenase hydroxylases.

  2011cites this paper
• Structural and catalytic properties of DNA/RNA-heme complexes

  2011cites this paper
• The many faces of the adamantyl group in drug design.

  2011cites this paper
• Targeting of the highly conserved threonine 302 residue of cytochromes P450 2B family during mechanism-based inactivation by aryl acetylenes.

  2011cites this paper
• The molecular biology, biochemistry, and physiology of human steroidogenesis and its disorders.

  2011cites this paper
• P450 enzymes: their structure, reactivity, and selectivity-modeled by QM/MM calculations.

  2010cites this paper
• Conformational Plasticity and Structure/Function Relationships in Cytochromes P450

  2010cites this paper
• Covalent Modification of Thr302 in Cytochrome P450 2B1 by the Mechanism-Based Inactivator 4-tert-Butylphenylacetylene

  2010cites this paper
• Scanning chimeragenesis: the approach used to change the substrate selectivity of fatty acid monooxygenase CYP102A1 to that of terpene ω-hydroxylase CYP4C7

  2010cites this paper
• S K-edge XAS and DFT calculations on cytochrome P450: covalent and ionic contributions to the cysteine-Fe bond and their contribution to reactivity.

  2009cites this paper
• Revisiting the mechanism of dioxygen activation in soluble methane monooxygenase from M. capsulatus (Bath): evidence for a multi-step, proton-dependent reaction pathway.

  2009cites this paper
• Role for threonine 201 in the catalytic cycle of the soluble diiron hydroxylase toluene 4-monooxygenase.

  2009cites this paper
• How is the reactivity of cytochrome P450cam affected by Thr252X mutation? A QM/MM study for X = serine, valine, alanine, glycine.

  2009cites this paper
• Biochemical and Spectroscopic Characterization of Tryptophan Oxygenation: Tryptophan 2, 3-Dioxygenase and Maug

  2009cites this paper
• Quantum mechanics/molecular mechanics studies on the sulfoxidation of dimethyl sulfide by compound I and compound 0 of cytochrome P450: which is the better oxidant?

  2009cites this paper
• Towards robust and specific enzymatic oxidation of hydrocarbons

  2009cites this paper
• New insights into the structural characteristics and functional relevance of the human cytochrome P450 2D6 enzyme

  2009cites this paper
• Efficient catalytic turnover of cytochrome P450(cam) is supported by a T252N mutation.

  2008cites this paper
• Structural rationalization of novel drug metabolizing mutants of cytochrome P450 BM3

  2008cites this paper
• Theoretical and Experimental Evaluation of a CYP106A2 Low Homology Model and Production of Mutants with Changed Activity and Selectivity of Hydroxylation

  2008cites this paper
• Oxygen activation by cytochrome P450 monooxygenase

  2008cites this paper
• Alkane hydroxylation by peroxy acids: a comparison with the cytochrome P450 hydroxylation.

  2008cites this paper
• Alteration of P450 distal pocket solvent leads to impaired proton delivery and changes in heme geometry.

  2007cites this paper
• Sterol 14alpha-demethylase cytochrome P450 (CYP51), a P450 in all biological kingdoms.

  2007cites this paper
• Sterol 14α-Demethylase Cytochrome P450 (CYP51), a P450 in all Biological Kingdoms

  2007cites this paper
• Proton assisted oxygen-oxygen bond splitting in cytochrome p450.

  2007cites this paper
• The molecular enzymology of cytochromes P450 of biotechnological and medical interest

  2007cites this paper
• The role of Thr268 and Phe393 in cytochrome P450 BM3.

  2006cites this paper
• Chapter 10 Cytochrome P450 Enzymes: Computational Approaches to Substrate Prediction

  2006cites this paper
• Engineering of Cytochrome P450 3A4 for Enhanced Peroxide-Mediated Substrate Oxidation Using Directed Evolution and Site-Directed Mutagenesis

  2006cites this paper
• Identification of Binding Sites of Non-I-Helix Water Molecules in Mammalian Cytochromes P450

  2006cites this paper
• Drug metabolism and chemotherapy resistance

  2006cites this paper
• Cytochromes P450 as versatile biocatalysts.

  2006cites this paper
• A 4 for Enhanced Peroxide-mediated Substrate Oxidation Using Directed Evolution and Site-directed Mutagenesis

  2006cites this paper
• Engineering of Cytochrome P 450 3 A 4 for Enhanced Peroxide-Mediated Substrate Oxidation Using Directed Evolution and Site-Directed Mutagenesis

  2006cites this paper
• Investigation of the role of cytochrome P450 2B4 active site residues in substrate metabolism based on crystal structures of the ligand-bound enzyme.

  2006cites this paper
• MECHANISM-BASED INACTIVATION AND REVERSIBILITY: IS THERE A NEW TREND IN THE INACTIVATION OF CYTOCHROME P450 ENZYMES?

  2006cites this paper