Absolute quantification of cassava brown streak virus mRNA by real-time qPCR

R. Shirima,Daniel G. Maeda,E. Kanju,Gloria Ceasar,F. Tibazarwa,J. Legg

Published 2017 in Journal of Virological Methods

ABSTRACT

Highlights • First protocol for absolute quantification of cassava brown streak viruses.• Standard templates for specific absolute quantification of CBSVs generated.• Acceptable standard curves for specific absolute quantification of CBSVs prepared.• Screening efficiency for CBSD-resistance sources will be greatly improved.

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