Towards an improved serum-free, chemically defined medium for long-term culturing of cerebral cortex tissue.

No abstract is available for this paper.

• Publication year

  1984

• Venue

  Neuroscience and Biobehavioral Reviews

• Publication date

  1984-09-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0149-7634(84)90055-1PMID 6504415
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Effects of essential fatty acid deficiency on the central nervous system in the growing rat. In: lipids, malnutrition & the developing brain.

  2008cited by this paper
• Hormonal growth control of cells in culture

  2007cited by this paper
• The effect of ascorbic acid on malonaldehyde formation, K+, Na+ and water content of brain slices

  2004cited by this paper
• Selective destruction of the outer leaflet of the capillary endothelial membrane after intracerebral injection of arachidonic acid in the rat

  2004cited by this paper
• An ultrastructural study of the maturation of neuronal somata in the visual cortex of the rat

  2004cited by this paper
• Lipid metabolism of developing central nervous tissues in organotypic cultures

  2004cited by this paper
• Effects of chronic vitamin E deficiency on the nervous system of the rat

  2004cited by this paper
• Neuropathology of chronic vitamine E deficiency in fatal familial intrahepatic cholestasis

  2004cited by this paper
• The postnatal development of synapses in the different laminae of the visual cortex in the normal kitten and in kittens with eyelid suture.

  1983cited by this paper
• Effect of Polyunsaturated Fatty Acids on Fetal Mouse Brain Cells in Culture in a Chemically Defined Medium

  1983cited by this paper
• Lipid Peroxide Distribution in Brain and the Effect of Hyperbaric Oxygen

  1983cited by this paper
• Trophic effects of brain areas on the developing cerebral cortex: I. Growth and histological organization of intraocular grafts.

  1983cited by this paper
• The effect of ascorbic acid on malonaldehyde formation, K+, Na+ and water content of brain slices.

  1983cited by this paper
• Effect of thyroid hormone on the synthesis of sialosyl galactosylceramide (GM4) in myelinogenic cultures of cells dissociated from embryonic mouse brain.

  1983cited by this paper
• Oxygen toxicity of hippocampal tissue in vitro.

  1983cited by this paper
• Age-dependent requirements of sympathetic neurons in serum-free culture.

  1983cited by this paper
• Induction of brain edema following intracerebral injection of arachidonic acid

  1983cited by this paper
• Myelin changes induced by incubation of brain slices with serum.

  1983cited by this paper
• Glucocorticoid regulation of synaptic development.

  1983cited by this paper
• Primary astrocyte cultures—a key to astrocyte function

  1983cited by this paper
• The concentration of retinoic acid determines the differentiated cell types formed by a teratocarcinoma cell line.

  1983cited by this paper
• Trophic effects of brain areas on the developing cerebral cortex: II. Electrophysiology of intraocular grafts.

  1983cited by this paper
• Insulin-like growth factor I (IGF I) stimulates DNA synthesis in fetal rat brain cell cultures.

  1983cited by this paper
• Synaptic activity mediates death of hypoxic neurons.

  1983cited by this paper
• Ascorbic acid could be hazardous to your experiments: a commentary on dopamine receptor binding studies with speculation on a role for ascorbic acid in neuronal function.

  1983cited by this paper
• Serum‐ and substratum‐dependent modulation of neuritic growth

  1983cited by this paper
• Effect of neonatal and adult-onset hypothyroidism on pyramidal cells of the rat auditory cortex.

  1983cited by this paper
• Lactate Uptake and Release in the Presence of Glucose by Sympathetic Ganglia of Chicken Embryos and by Neuronal and Nonneuronal Cultures Prepared from These Ganglia

  1983cited by this paper
• Effects of thyroid deficiency and corrective effects of thyroxine on microtubules and mitochondria in cerebellar Purkinje cell dendrites of developing rats

  1983cited by this paper
• Local differences in the amount of early cell death in neocortex predict adult local specializations.

  1983cited by this paper
• Cholinergic neurons in the rat cerebral cortex demonstrated by immunohistochemical localization of choline acetyltransferase.

  1983cited by this paper
• Transferrin as a fetal growth factor: acquisition of responsiveness related to embryonic induction.

  1983cited by this paper
• Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells

  1982cited by this paper
• Ascorbic Acid in Neural Tissues

  1982cited by this paper
• Effects of retinoic acid on the binding and mitogenic activity of epidermal growth factor

  1982cited by this paper
• Investigations on Myelination In Vitro: Regulation of 2′,3′‐Cyclic Nucleotide 3′‐Phosphohydrolase by Thyroid Hormone in Cultures of Dissociated Brain Cells from Embryonic Mice

  1982cited by this paper
• Detailed mapping of ascorbate distribution in rat brain.

  1982cited by this paper
• Alterations of Nuclear Thyroid Hormone Receptors in Cerebral Cortex In Vivo

  1982cited by this paper
• Effects of prenatal progesterone on the development of pyramidal cells in rat cerebral cortex.

  1982cited by this paper
• Formation of postsynaptic specializations induced by latex beads in cultured muscle cells

  1982cited by this paper
• The distribution and role of carnitine in the mammalian brain.

  1982cited by this paper
• Effects of cell density on the neutral glycolipid composition of cultured human brain and glioma cells

  1982cited by this paper
• Limiting section thickness of guinea pig olfactory cortical slices studied from tissuepO2 values and electrical activities

  1982cited by this paper
• THE EFFECTS OF VITAMIN E ON ARACHIDONIC ACID METABOLISM *

  1982cited by this paper
• KINETICS OF TISSUE α‐TOCOPHEROL UPTAKE AND DEPLETION FOLLOWING ADMINISTRATION OF HIGH LEVELS OF VITAMIN E

  1982cited by this paper
• Influence of growth medium, age in vitro and spontaneous bioelectric activity on the distribution of sensory ganglion-evoked activity in spinal cord explants.

  1982cited by this paper
• Thyroxine treatment and recovery of hypothyroidism-induced pyramidal cell damage.

  1982cited by this paper
• Electrical stimulation of hindlimb increases neuronal cell death in chick embryo

  1982cited by this paper
• Effects of vitamin A on the behaviour of migratory neural crest cells in vitro.

  1982cited by this paper
• Effects of corticosterone on brain cholinergic enzymes in chick embryos

  1982cited by this paper
• Detailed mapping of ascorbate distribution in rat brain.

  1982cited by this paper
• The role of transferrin in the growth of testicular cell lines in serum-free medium.

  1982cited by this paper
• Free radical formation and lipid peroxidation in rat and mouse cerebral cortex slices exposed to high oxygen pressure.

  1982cited by this paper
• Nerve growth factor (NGF) stimulation of cholinergic telencephalic neurons in aggregating cell cultures.

  1982cited by this paper
• Serum contains a platelet-derived transforming growth factor.

  1982cited by this paper
• Synaptogenesis in human visual cortex--evidence for synapse elimination during normal development.

  1982cited by this paper
• A method for large-scale production of mouse brain cortical cultures.

  1982cited by this paper
• Factors affecting the free fatty acids in rat brain cortex.

  1982cited by this paper
• Intracellular cysteine delivery system that protects against toxicity by promoting glutathione synthesis.

  1982cited by this paper
• Vitamin A concentration in amniotic fluid and maternal serum related to neural‐tube defects

  1982cited by this paper
• FREE RADICAL BIOLOGY: XENOBIOTICS, CANCER, AND AGING *

  1982cited by this paper
• Effects of deoxycorticosterone and its ring A-reduced derivatives on the nervous system.

  1982cited by this paper
• Effect of hydrocortisone and thyroxine on ATPase activities of neuronal and glial cell lines in culture

  1982cited by this paper
• In vitro cellular respiration at elevated temperatures in developing rat cerebral cortex.

  1982cited by this paper
• Homeostatic control of ascorbate concentration in CNS extracellular fluid.

  1982cited by this paper
• AN UPDATE ON ANTIOXIDANT THEORY: SPIN TRAPPING OF TRICHLOROMETHYL RADICALS IN VIVO

  1982cited by this paper
• Postnatal changes in the number of neurons and synapses in the visual cortex (area 17) of the macaque monkey: A stereological analysis in normal and monocularly deprived animals

  1982cited by this paper
• CHAPTER 7 – Glutathione Peroxidase Brought into Focus

  1982cited by this paper
• The aging process.

  1981cited by this paper
• Organotypic monolayer cultures of nervous tissue.

  1981cited by this paper
• Ascorbic acid inhibition of Na,K-adenosine triphosphatase of rat forebrain without peroxidation of membrane lipids.

  1981cited by this paper
• Synthesis of lipids in mouse brain cell cultures during development

  1981cited by this paper
• Rapid clonal growth and serial passage of human diploid fibroblasts in a lipid-enriched synthetic medium supplemented with epidermal growth factor, insulin, and dexamethasone.

  1981cited by this paper
• Synapse replacement in the nervous system of adult vertebrates.

  1981cited by this paper
• Investigations on myelination in vitro. Regulation of sulfolipid synthesis by thyroid hormone in cultures of dissociated brain cells from embryonic mice.

  1981cited by this paper
• Progesterone receptors in the cerebral cortex of neonatal female rats.

  1981cited by this paper
• Regional distribution of ascorbate in human brain.

  1981cited by this paper
• Differentiation of fetal mouse hypothalamic cells in serum-free medium.

  1981cited by this paper
• NEURONAL DEATH IN THE DEVELOPMENT AND AGING OF THE CEREBRAL CORTEX OF THE MOUSE

  1981cited by this paper
• Glial-released proteins: II. Two-dimensional electrophoretic identification of proteins regulated by hydrocortisone.

  1981cited by this paper
• Nerve outgrowth, synaptogenesis and bioelectric activity in fetal rat cerebral cortex tissue cultured in serum-free, chemically defined medium.

  1981cited by this paper
• Effects of postdecapitative ischemia on arachidonate release from brain synaptosomes

  1981cited by this paper
• 9 EFFECTS OF EXOGENOUS GANGLIOSIDES ON GROWTH OF CULTURED GLIOMA AND FETAL BRAIN CELLS

  1981cited by this paper
• Metabolic and functional defects in selenium deficiency.

  1981cited by this paper
• Chemically defined medium enhances bioelectric activity in mouse spinal cord-dorsal root ganglion cultures.

  1981cited by this paper
• Insulin acts as a somatomedin analog in stimulating myoblast growth in serum-free medium

  1981cited by this paper
• The Uptake of Carnitine by Slices of Rat Cerebral Cortex

  1981cited by this paper
• Growth of dissociated rat cerebellar cells using serum-free supplemented media and varied transferrin concentrations

  1981cited by this paper
• A quantitative electron microscopic study on synapse formation in dissociated fetal rat cerebral cortex in vitro.

  1981cited by this paper
• Polyamine metabolism and function in brain.

  1981cited by this paper
• Ascorbic acid enables reversible dopamine receptor 3H-agonist binding.

  1981cited by this paper
• Effects of insulin on cellular growth and proliferation.

  1981cited by this paper
• In vitro and in vivo depolarization coupled efflux of ascorbic acid in rat brain preparations.

  1981cited by this paper
• Insulin receptors, receptor antibodies, and the mechanism of insulin action.

  1981cited by this paper
• Alteration of the action potential of tissue cultured neuronal cells by growth in the presence of a polyunsaturated fatty acid

  1981cited by this paper
• Progressive Neuropathologic Lesions in Vitamin E-Deficient Rhesus Monkeys

  1981cited by this paper
• Effects of Insulin on Cultured Rat Brain Cells: Stimulation of Ornithine Decarboxylase Activity

  1981cited by this paper
• Vitamin E-enriched lipoproteins increase longevity of neurons in vitro.

  1981cited by this paper
• Maturation of rat visual cortex. I. A quantitative study of Golgi-impregnated pyramidal neurons

  1981cited by this paper
• ACTION OF RETINOIDS AND PHORBOL ESTERS ON CELL GROWTH AND THE BINDING OF EPIDERMAL GROWTH FACTOR

  1981cited by this paper

• Oxygen gradient generator to improve in vitro modeling of ischemic stroke

  2023cites this paper
• Prediction in cultured cortical neural networks

  2023cites this paper
• The Association between Hypoxia-Induced Low Activity and Apoptosis Strongly Resembles That between TTX-Induced Silencing and Apoptosis

  2022cites this paper
• Ghrelin Regulates Expression of the Transcription Factor Pax6 in Hypoxic Brain Progenitor Cells and Neurons

  2022cites this paper
• Enteric Neural Network Assembly Was Promoted by Basic Fibroblast Growth Factor and Vitamin A but Inhibited by Epidermal Growth Factor

  2022cites this paper
• Maximum entropy models provide functional connectivity estimates in neural networks

  2022cites this paper
• Evidence for accelerated aging in mammary epithelia of women carrying germline BRCA1 or BRCA2 mutations

  2021cites this paper
• Mild stimulation improves neuronal survival in an in vitro model of the ischemic penumbra

  2019cites this paper
• Nanogroove-Enhanced Hydrogel Scaffolds for 3D Neuronal Cell Culture: An Easy Access Brain-on-Chip Model

  2019cites this paper
• Different Conformational Subensembles of the Intrinsically Disordered Protein α-Synuclein in Cells

  2018cites this paper
• Nanoscale membrane actuator for in vitro mechano-stimuli responsive studies of neuronal cell networks on chip

  2018cites this paper
• Evolution of Excitation–Inhibition Ratio in Cortical Cultures Exposed to Hypoxia

  2018cites this paper
• Exogenous α-synuclein hinders synaptic communication in cultured cortical primary rat neurons

  2018cites this paper
• Loss and recovery of functional connectivity in cultured cortical networks exposed to hypoxia.

  2017cites this paper
• Dual effect of fetal bovine serum on early development depends on stage-specific reactive oxygen species demands in pigs

  2017cites this paper
• Reduced Synaptic Vesicle Recycling during Hypoxia in Cultured Cortical Neurons

  2017cites this paper
• Rapid solution exchange to entire neuronal cultures grown on multi electrode arrays

  2017cites this paper
• The number of α-synuclein proteins per vesicle gives insights into its physiological function

  2016cites this paper
• Functionally different α-synuclein inclusions yield insight into Parkinson’s disease pathology

  2016cites this paper
• Mitochondrial dysfunction precedes depression of AMPK/AKT signaling in insulin resistance induced by high glucose in primary cortical neurons

  2016cites this paper
• Microfabrication and microfluidics for 3D brain-on-chip

  2016cites this paper
• Transcriptomic Profiling of Mice Primary Cortical Neurons in Response to Medium Change

  2016cites this paper
• Fabrication and characterization of microsieve electrode array (µSEA) enabling cell positioning on 3D electrodes

  2016cites this paper
• Progression of Neuronal Damage in an In Vitro Model of the Ischemic Penumbra

  2016cites this paper
• Acyl Ghrelin Improves Synapse Recovery in an In Vitro Model of Postanoxic Encephalopathy

  2015cites this paper
• Repeated stimulation of cultured networks of rat cortical neurons induces parallel memory traces

  2015cites this paper
• Media formulation influences chemical effects on neuronal growth and morphology

  2015cites this paper
• Tonic GABAergic Inhibition of Sympathetic Preganglionic Neurons: A Novel Substrate for

  2015cites this paper
• Advances in 3D neuronal cell culture

  2015cites this paper
• Barbed channels enhance unidirectional connectivity between neuronal networks cultured on multi electrode arrays

  2015cites this paper
• Nanoscaffold's stiffness affects primary cortical cell network formation

  2014cites this paper
• Connectivity, excitability and activity patterns in neuronal networks

  2014cites this paper
• Ghrelin accelerates synapse formation and activity development in cultured cortical networks

  2014cites this paper
• Mild hypoxia affects synaptic connectivity in cultured neuronal networks.

  2014cites this paper
• Imprint lithography provides topographical nanocues to guide cell growth in primary cortical cell culture

  2014cites this paper
• Hydrogel/poly-dimethylsiloxane hybrid bioreactor facilitating 3D cell culturing

  2013cites this paper
• Tetanic stimulation of cortical networks induces parallel memory traces in experimental cultures and computer models

  2013cites this paper
• Strategies for repair of white matter: influence of osmolarity and microglia on proliferation and apoptosis of oligodendrocyte precursor cells in different basal culture media

  2013influential citation
• Ghrelin stimulates synaptic formation in cultured cortical networks in a dose-dependent manner.

  2013cites this paper
• pierwotna hodowla komórek nerwowych hipokampa – zastosowanie metody w modelach neuroprotekcji doświadczalnej primary culture of dissociated hippocampal neurons – the use of method in the experimental models of neuroprotection

  2011cites this paper
• Neural cell–cell and cell–substrate adhesion through N-cadherin, N-CAM and L1

  2011influential citation
• Development of an 11000 electrode high-density multi- electrode-array system for neural network recording

  2011cites this paper
• Single pulse responses in cultured neuronal networks to describe connectivity

  2011cites this paper
• Advances in Anatomy Embryology and Cell Biology

  2011cites this paper
• Orexin A in Cortical Cultures: Expression and Effect on Synaptogenesis During Development

  2011cites this paper
• Experimental analysis and computational modeling of interburst intervals in spontaneous activity of cortical neuronal culture

  2011cites this paper
• Bifurcating microchannels as a scaffold to induce separation of regenerating neurites

  2010cites this paper
• Impedance Sensing for Monitoring Neuronal Coverage and Comparison With Microscopy

  2010cites this paper
• Phase-dependent effects of stimuli locked to oscillatory activity in cultured cortical networks.

  2010cites this paper
• Perforated Multielectrode Array in Drug Discovery

  2010influential citation
• Regulation of in vitro cell-cell and cell-substrate adhesion

  2010influential citation
• In vitro Verification of a 3-D Regenerative Neural Interface Design: Examination of Neurite Growth and Electrical Properties Within a Bifurcating Microchannel Structure

  2010cites this paper
• Inhibition of neuronal cell–cell adhesion measured by the microscopic aggregation assay and impedance sensing

  2010cites this paper
• The Effect of Slow Electrical Stimuli to Achieve Learning in Cultured Networks of Rat Cortical Neurons

  2010cites this paper
• In vitro Verification of a 3-D Regenerative Neural Interface Design: Examination of Neurite GrowthandElectricalProperties Within a Bifurcating Microchannel Structure Implantable devices that guide growth of multiple neurites are used to prompt cell separation and improve selective neural interfaces.

  2010cites this paper
• Spontaneous and synchronous firing activity in solitary microcultures of cortical neurons on chemically patterned multielectrode arrays

  2009cites this paper
• Slow electrical stimuli affect connectivity in cultured neuronal networks

  2009cites this paper
• Live learning neuronal networks : plasticity of bursts

  2009cites this paper
• Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex.

  2009cites this paper
• Aberrant luminal progenitors as the candidate target population for basal tumor development in BRCA1 mutation carriers

  2009cites this paper
• Neural growth into a microchannel network: Towards A regenerative neural interface

  2009cites this paper
• The Effect of Learning on Bursting

  2009cites this paper
• Do external stimuli, applied to train cultured cortical networks, disturb the balance between activity and connectivity?

  2008cites this paper
• Analysis of Cultured Neuronal Networks Using Intraburst Firing Characteristics

  2008cites this paper
• Secretion of 23 kDa and glycosylated prolactin by rat pituitary cell culture in serum-free media: a comparative morphological, cyto- and immunochemical study

  2008cites this paper
• NS21: re-defined and modified supplement B27 for neuronal cultures.

  2008cites this paper
• Changes within bursts during learning in dissociated neural networks

  2008cites this paper
• β-Amyloid precursor protein (APP) and APP-RNA are rapidly affected by glutamate in cultured neurons

  2007cites this paper
• A new hybrid neural system interfacing neurons and silicon hardware for fast signal recognition

  2005cites this paper
• K+ conductance of mechanically dissociated rat sensory neurones in long lerm cultures in a defined medium

  2004cites this paper
• Rapid Communication Optimized Survival of Hippocampal Neurons in B 27-Supplemented Neurobasalm , a New Serum-free Medium Combination

  2004cites this paper
• GABAA receptor maturation in relation to eye opening in the rat visual cortex.

  2004cites this paper
• Development in the absence of spontaneous bioelectric activity results in increased stereotyped burst firing in cultures of dissociated cerebral cortex

  2004cites this paper
• Differential antiepileptic effects of the organic calcium antagonists verapamil and flunarizine in neurons of organotypic neocortical explants from newborn rats

  2004cites this paper
• Influence of meningeal cells on the proliferation and maturation of rat neuroblasts in culture

  2004influential citation
• Long-term adhesion and survival of dissociated cortical neurons on miniaturised chemical patterns

  2003cites this paper
• Cross-interval histogram analysis of neuronal activity on multi-electrode arrays

  2003cites this paper
• Low Frequency Changes in Skin Surface Potentials by Skin Compression: Experimental Results and Theories

  2003cites this paper
• Differential effects of ethanol antagonism and neuroprotection in peptide fragment NAPVSIPQ prevention of ethanol-induced developmental toxicity

  2003cites this paper
• "We dance round in a ring and suppose, but the secret sits in the middle and knows."

  2003cites this paper
• Allopregnanolone activates GABA(A) receptor/Cl(-) channels in a multiphasic manner in embryonic rat hippocampal neurons.

  2002influential citation
• Investigating membrane breakdown of neuronal cells exposed to nonuniform electric fields by finite-element modeling and experiments

  2002influential citation
• The influence of carbon dioxide concentration on the neurite outgrowth of mouse embryonic contical neurons in vitro.

  2002cites this paper
• Survival at low and growth of hippocampal neurons in defined medium density : advantages of a sandwich culture technique or low oxygen

  2002cites this paper
• Immortalized chromaffin cells disimmortalized with Cre/lox site-directed recombination for use in cell therapy for pain after partial nerve injury.

  2002cites this paper
• Chemokine release is associated with the protective action of PACAP-38 against HIV envelope protein neurotoxicity.

  2002cites this paper
• Intrinsic Properties Inhibit Axonal Outgrowth from Neonatal Rat Spinal Cord Explant

  2002cites this paper
• Extracellular detection of active membrane currents in the neuron-electrode interface.

  2002cites this paper
• Pax6 is required to regulate the cell cycle and the rate of progression from symmetrical to asymmetrical division in mammalian cortical progenitors.

  2002influential citation
• Allopregnanolone Activates GABAA Receptor/Cl Channels in a Multiphasic Manner in Embryonic Rat Hippocampal Neurons

  2002influential citation
• Allopregnanolone Activates GABAAReceptor/Cl− Channels in a Multiphasic Manner in Embryonic Rat Hippocampal Neurons

  2002cites this paper
• Experimental Investigation on Neural Cell Survival after Dielectrophoretic Trapping

  2002influential citation
• Adhesion and growth of electrically active cortical neurons on polyethylenimine patterns microprinted onto PEO-PPO-PEO triblockcopolymer-coated hydrophobic surfaces.

  2002cites this paper
• Profuse and selective growth in vitro of rat spinal axons on a micro-patterned poly (ethylene imine) grid

  2001cites this paper
• Lumbar spinal cord explants from neonatal rat display age-related decrease of outgrowth in culture.

  2001cites this paper
• Neuroelectronic interfacing with cultured multielectrode arrays toward a cultured probe

  2001cites this paper
• Viability of dielectrophoretically trapped neural cortical cells in culture.

  2001cites this paper
• Dielectrophoretic trapping of dissociated fetal cortical rat neurons

  2001cites this paper
• Glutamate receptor subunit expression in primary neuronal and secondary glial cultures

  2001influential citation
• Astrocytes release small molecules to protect neurons from oxidative stress

  2001cites this paper