The influence of calcium-free EGTA solution upon membrane permeability in the crystalline lens of the frog

Potential difference, resistance, cation content, and 86Rb efflux were measured in frog lenses maintained in normal or calcium-free EGTA Ringer's solution. Exposure of the lens to calcium-free solution resulted in a rapid fall in potential and resistance, together with a twofold increase in 86Rb efflux rate. These rapid changes were not due to an alteration in cation distribution between the lens and its environment. However, the alteration in 86Rb efflux rate could be explained on the basis of the fall in potential. These findings suggested that removal of calcium from the bathing medium caused a rapid increase in sodium permeability alone. This suggestion was substantiated by the results of experiments where the response of the lens to low calcium solution was determined in a medium in which 90% of the sodium had been replaced by sucrose.

• Publication year

  1978

• Venue

  The Journal of General Physiology

• Publication date

  1978-05-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1085/JGP.71.5.581PMID 307048PMCID 2215107
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Electrophysiology of the Mammalian Lens

  1978cited by this paper
• A comparison of ion concentrations, potentials and conductances of amphibian, bovine and cephalopod lenses

  1977influential reference
• Distribution of the extracellular space of the amphibian lens.

  1976cited by this paper
• Current‐voltage relationships in the crystalline lens.

  1976influential reference
• Letter: The potential difference of the rabbit lens.

  1974cited by this paper
• Intracellular potassium activity in frog lens determined using ion specific liquid ion-exchanger filled microelectrodes.

  1974cited by this paper
• Voltage compartments in the lens.

  1974cited by this paper
• The potential difference of the rabbit lens

  1974cited by this paper
• Relative permeabilities of the lens membranes to sodium and potassium.

  1969cited by this paper
• The site of the ion restricting membranes in the toad lens.

  1969cited by this paper
• The Role of Calcium in the Regulation of the Steady-State Levels of Sodium and Potassium in the HeLa Cell

  1967cited by this paper
• THE EFFLUX OF 86-RUBIDIUM FROM THE RABBIT LENS.

  1965cited by this paper
• THE ELECTRON MICROSCOPY OF THE NORMAL HUMAN LENS.

  1965cited by this paper
• THE EFFECT OF CALCIUM ON RAT LENS PERMEABILITY.

  1965cited by this paper
• Changes in the Membrane Permeability of Frog's Sartorius Muscle Fibers in Ca-Free EDTA Solution

  1963influential reference
• The Gross Anatomy and Embryology of the Eye

  1962cited by this paper
• The Effect of Calcium on Sodium Transport by Frog Skin

  1962cited by this paper
• Accumulation of rubidium-86 by the rabbit lens.

  1962cited by this paper
• The Effect of Calcium on the Cations of Calf Lens

  1960cited by this paper
• Significance of changes in potassium and sodium content of the lens. A mechanism for lenticular intumescence.

  1951cited by this paper
• The effect of sodium ions on the electrical activity of the giant axon of the squid

  1949cited by this paper
• POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANES

  1943cited by this paper
• Lens Opacities Associated with Experimental Calcium Deficiency*: Preliminary report

  1941cited by this paper
• THE INFLUENCE OF CALCIUM DEFICIENCY ON THE SURVIVING RABBIT'S LENS: AN EXPERIMENTAL STUDY ON THE PATHOGENESIS OF CATARACTA TETANICA

  1940cited by this paper
• The Relation of the Parathyroid Gland to Cataract

  1931cited by this paper

• Cataract and optic disk drusen in a patient with glycogenosis and di George syndrome: clinical and molecular report

  2017cites this paper
• A permeability barrier surrounds taste buds in lingual epithelia.

  2015cites this paper
• Elevation of intracellular Zn2+ level by nanomolar concentrations of triclocarban in rat thymocytes.

  2012cites this paper
• Biology of the Lens: Lens Transparency as a Function of Embryology, Anatomy, and Physiology

  2008cites this paper
• Imidazole antifungals, but not triazole antifungals, increase membrane Zn2+ permeability in rat thymocytes Possible contribution to their cytotoxicity.

  2008cites this paper
• Effects of A23187 and CaCl(2) on tri-n-butyltin-induced cell death in rat thymocytes.

  2003cites this paper
• Influence of amphotericin B on the sodium pump of porcine lens epithelium.

  1996cites this paper
• Effects of Ca2+ on rabbit translens short-circuit current: evidence for a Ca2+ inhibitable K+ conductance.

  1996cites this paper
• Is there a calcium paradox in isolated bovine aortic endothelial cells?

  1992cites this paper
• Senile cataract‐a biochemical approach and in vivo regulation of calcium and phosphate

  1992cites this paper
• Divalent cation effects on lens conductance and stretch-activated cation channels.

  1992cites this paper
• Increased ion traffic through non-specific cation pathways in the ageing human lens. Evidence from radiotracer fluxes studies.

  1991cites this paper
• Nutritional factors in cataract.

  1990cites this paper
• EGTA modulates equatorial currents in frog lenses.

  1989cites this paper
• Membrane and junctional properties of the isolated frog lens epithelium

  1988cites this paper
• The calcium paradox of the heart.

  1987cites this paper
• The Cellular Basis of Ischemic Acute Renal Failure

  1986cites this paper
• Membrane permeability characteristics of perfused human senile cataractous lenses.

  1986cites this paper
• Examination of lens calcium balance using 45Ca uptake studies.

  1985cites this paper
• Response of the intracellular potentials of cultured bovine lens cells to ions and inhibitors.

  1985cites this paper
• Effects of calcium on the steady outward currents at the equator of the rat lens.

  1985cites this paper
• Lenticular energy metabolism during exogenous calcium deprivation and during recovery: effects of dextran-40.

  1985cites this paper
• Mechanism of depolarization of rat cortical synaptosomes at submicromolar external Ca2+ activity. The use of Ca2+ buffers to control the synaptosomal membrane potential.

  1985cites this paper
• Characteristics of 45Ca uptake by the rabbit lens.

  1985cites this paper
• 86-Rb efflux in normal and cataractous human lenses.

  1985cites this paper
• Localization of potassium and sodium fluxes in the rat lens.

  1985cites this paper
• A quantitative microprobe analysis of elements in cortical and nuclear cells of the calf lens

  1985cites this paper
• Chapter 2 – The Lens as a Physicochemical System

  1984cites this paper
• Activator of Ca2+-transport in the lens.

  1984cites this paper
• The Bovine Lens as a Model for Osmotic Cataract

  1983cites this paper
• Lens cation transport and permeability changes following exposure to hydrogen peroxide.

  1983cites this paper
• Chlorpromazine effects upon rabbit lens water and electrolyte balance.

  1983cites this paper
• Calcium metabolism of the rabbit lens.

  1983cites this paper
• Lens permeability changes associated with metabolic inhibition by iodoacetate.

  1982cites this paper
• The effect of 2-4 dinitrophenol on cell to cell communication in the frog lens.

  1982cites this paper
• Studies on calcium regulation in relation to sodium-potassium balance in the rabbit lens.

  1982cites this paper
• Effects of extracellular calcium removal and anoxia on isolated rat myocytes.

  1982cites this paper
• An analysis of 45Ca fluxes in the rabbit lens.

  1982cites this paper
• The effect of 2–4 dinitrophenol on cell to cellcommunication in the frog lens

  1982cites this paper
• Effects of veratrine and tetrodotoxin on the frog lens potential in normal and calcium-free media

  1982cites this paper
• Calcium controls both sodium and potassium permeability of lens membranes.

  1981cites this paper
• Hypocalcemic cataract. I. An animal model and cation distribution study.

  1981cites this paper
• Impedance of the amphibian lens.

  1981cites this paper
• The calcium metabolism of the amphibian lens.

  1980cites this paper
• Cortical opacity, calcium concentration and fiber membrane structure in the calf lens.

  1980cites this paper
• The influence of external potassium ions upon lens conductance characteristics investigated using a voltage clamp technique.

  1980cites this paper
• Some observations on the magnesium metabolism of the amphibian lens.

  1980cites this paper
• The influence of calcium-free media on the electrical properties of the isolated toad lens.

  1980cites this paper
• Contribution of an electrogenic pump to the electrical characteristics of frog lens membranes.

  1980cites this paper
• Relative roles of Ca2+, Sr2+, Ba2+ and Mg2+ in controlling lens permeability characteristics

  1980cites this paper
• Observations on high and low voltage compartments in the crystalline lens of the frog.

  1979cites this paper
• The viability of the bovine lens in organ culture.

  1979cites this paper
• The influence of calcium-free solutions upon permeability characteristics of the rabbit lens.

  1979cites this paper
• Dextran's effects on stressed lenses: water, electrolyte, and radioisotope studies.

  1979cites this paper