Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli.

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Metabolic Engineering

• Publication date

  2011-05-01

• Fields of study

  Biology, Chemistry, Engineering, Environmental Science, Medicine

• Identifiers
  DOI 10.1016/j.ymben.2011.02.004PMID 21515217
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2009influential reference
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  2008influential reference
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  2008cited by this paper
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  2008influential reference
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  2005influential reference
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  2004influential reference
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  1997influential reference
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  1992influential reference
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  1989influential reference
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  1987cited by this paper
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