Drought and heat are among the major abiotic stresses that affect soybean crops worldwide. During the current investigation, the effect of drought, heat, and drought plus heat stresses was compared in the leaves of two soybean varieties, Surge and Davison, combining 2D-DIGE proteomic data with physiology and biochemical analyses. We demonstrated how 25 differentially expressed photosynthesis-related proteins affect RuBisCO regulation, electron transport, Calvin cycle, and carbon fixation during drought and heat stress. We also observed higher abundance of heat stress-induced EF-Tu protein in Surge. It is possible that EF-Tu might have activated heat tolerance mechanisms in the soybean. Higher level expressions of heat shock-related protein seem to be regulating the heat tolerance mechanisms. This study identifies the differential expression of various abiotic stress-responsive proteins that regulate various molecular processes and signaling cascades. One inevitable outcome from the biochemical and proteomics assays of this study is that increase of ROS levels during drought stress does not show significant changes at the phenotypic level in Davison and this seems to be due to a higher amount of carbonic anhydrase accumulation in the cell which aids the cell to become more resistant to cytotoxic concentrations of H2O2.
Leaf Proteome Analysis Reveals Prospective Drought and Heat Stress Response Mechanisms in Soybean
Aayudh Das,Moustafa M. Eldakak,Bimal Paudel,D. Kim,Homa Hemmati,Chhandak Basu,J. S. Rohila
Published 2016 in BioMed Research International
ABSTRACT
PUBLICATION RECORD
- Publication year
2016
- Venue
BioMed Research International
- Publication date
2016-03-13
- Fields of study
Biology, Agricultural and Food Sciences, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- 2d-dige proteomics
A gel-based proteomic method used to compare leaf protein abundance across treatments and soybean varieties.
Aliases: 2D-DIGE, two-dimensional difference gel electrophoresis
- carbonic anhydrase
An enzyme that interconverts carbon dioxide and bicarbonate in leaf cells.
- drought stress
A water-deficit stress condition applied to soybean leaves in the comparison.
- ef-tu protein
Elongation factor Tu, a protein identified among the heat-responsive leaf proteins.
Aliases: elongation factor Tu
- heat shock-related protein
A stress-associated protein family involved in the cellular heat-shock response.
Aliases: heat shock protein, HSP
- heat stress
An elevated-temperature stress condition applied to soybean leaves in the comparison.
- photosynthesis-related proteins
Proteins in soybean leaves that participate in photosynthetic machinery and carbon assimilation.
- soybean varieties surge and davison
The two soybean cultivars compared for their leaf proteome responses to drought and heat stress.
Aliases: Surge, Davison