Cloning of two novel human importin‐α subunits and analysis of the expression pattern of the importin‐α protein family

The import of many proteins into the nucleus is mediated by the importin‐α/β heterodimer. While only one importin‐β gene has been found, several forms of importin‐α have been described. In addition to the three human importin‐αs already identified, we report here the primary structure of two new human importin‐α proteins. The five known human importin‐α subunits can be classified into three subfamilies that appear conserved in higher eukaryotic organisms. We show by immunoblotting that the different importin‐α subfamilies are expressed in a variety of human tissues and mammalian cell lines.

• Publication year

  1997

• Venue

  FEBS Letters

• Publication date

  1997-11-03

• Fields of study

  Biology

• Identifiers
  DOI 10.1016/S0014-5793(97)01265-9
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Nuclear protein import.

  1997cited by this paper
• Differential Expression and Sequence-specific Interaction of Karyopherin α with Nuclear Localization Sequences*

  1997cited by this paper
• Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL.

  1997cited by this paper
• The conserved amino‐terminal domain of hSRP1 alpha is essential for nuclear protein import.

  1996cited by this paper
• The Nuclear Localization Signal of Lymphoid Enhancer Factor-1 Is Recognized by Two Differentially Expressed Srp1-Nuclear Localization Sequence Receptor Proteins (*)

  1996cited by this paper
• A 41 amino acid motif in importin‐alpha confers binding to importin‐beta and hence transit into the nucleus.

  1996cited by this paper
• In vivo evidence for involvement of a 58 kDa component of nuclear pore‐targeting complex in nuclear protein import.

  1995cited by this paper
• The overgrown hematopoietic organs-31 tumor suppressor gene of Drosophila encodes an Importin-like protein accumulating in the nucleus at the onset of mitosis

  1995cited by this paper
• Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62

  1995cited by this paper
• Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins.

  1995cited by this paper
• The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile.

  1995cited by this paper
• Distinct functions for the two importin subunits in nuclear protein import

  1995cited by this paper
• Sequence and characterization of cytoplasmic nuclear protein import factor p97

  1995cited by this paper
• Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope.

  1995cited by this paper
• Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins.

  1995cited by this paper
• A Karyophilic Protein Forms a Stable Complex with Cytoplasmic Components Prior to Nuclear Pore Binding (*)

  1995cited by this paper
• Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences.

  1995cited by this paper
• Purification of a Ran-interacting protein that is required for protein import into the nucleus.

  1994cited by this paper
• RAG-1 interacts with the repeated amino acid motif of the human homologue of the yeast protein SRP1.

  1994cited by this paper
• Isolation of a protein that is essential for the first step of nuclear protein import.

  1994cited by this paper
• Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein.

  1994cited by this paper
• The GTP-binding protein Ran/TC4 is required for protein import into the nucleus

  1993cited by this paper
• Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor [published erratum appears in J Cell Biol 1994 Jan;124(1-2):217]

  1993cited by this paper
• A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation.

  1992cited by this paper
• Cloning and characterization of SRP1, a suppressor of temperature-sensitive RNA polymerase I mutations, in Saccharomyces cerevisiae

  1992cited by this paper
• Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import.

  1991cited by this paper
• Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors

  1990cited by this paper
• Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.

  1988cited by this paper
• Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.

  1988cited by this paper
• Movement of a karyophilic protein through the nuclear pores of oocytes

  1984cited by this paper
• A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.

  1982cited by this paper

• Deficiency in KPNA4, but Not in KPNA3, Causes Attention Deficit/Hyperactivity Disorder like Symptoms in Mice

  2025cites this paper
• Drosophila Importin Alpha 1 (Dα1) Is Required to Maintain Germline Stem Cells in the Testis Niche

  2024cites this paper
• Normal male fertility in a mouse model of KPNA2 deficiency

  2024cites this paper
• Structural basis for nuclear import of adeno-associated virus serotype 6 capsid protein

  2024cites this paper
• Distribution of importin-α isoforms in poultry species and their tissue- and age-related differences.

  2023cites this paper
• LncRNA SNHG6 Upregulates KPNA5 to Overcome Gemcitabine Resistance in Pancreatic Cancer via Sponging miR-944

  2023cites this paper
• Structural characterization of human importin alpha 7 in its cargo-free form at 2.5 Å resolution

  2022cites this paper
• Decoding nonspecific interactions between human nuclear transport proteins: A computational study

  2021cites this paper
• Molecular profiling of nucleocytoplasmic transport factor genes in breast cancer

  2021cites this paper
• Importin α phosphorylation promotes TPX2 activation by GM130 to control astral microtubules and spindle orientation

  2021cites this paper
• Measuring and Interpreting Nuclear Transport in Neurodegenerative Disease—The Example of C9orf72 ALS

  2021cites this paper
• Kpna6 deficiency causes infertility in male mice by disrupting spermatogenesis

  2021cites this paper
• PACS‐1 contains distinct motifs for nuclear‐cytoplasmic transport and interacts with the RNA‐binding protein PTBP1 in the nucleus and cytosol

  2021cites this paper
• An epilepsy-associated mutation in the nuclear import receptor KPNA7 reduces nuclear localization signal binding

  2020cites this paper
• Role of Nucleoporins and Transport Receptors in Cell Differentiation

  2020cites this paper
• Importin α7 deficiency causes infertility in male mice by disrupting spermatogenesis

  2020cites this paper
• Cellular Importin-α3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection

  2020cites this paper
• Zika virus NS2A protein induces the degradation of KPNA2 (karyopherin subunit alpha 2) via chaperone-mediated autophagy

  2020cites this paper
• Evidence of nuclear transport mechanisms in the protozoan parasite Giardia lamblia.

  2019cites this paper
• Duck karyopherin α4 (duKPNA4) is involved in type I interferon expression and the antiviral response against Japanese encephalitis virus

  2019cites this paper
• Nucleocytoplasmic Shuttling of STATs. A Target for Intervention?

  2019cites this paper
• Characterization of the Importin-β binding domain in nuclear import receptor KPNA7.

  2019cites this paper
• The Extended C-Terminal Region of Influenza C Virus Nucleoprotein Is Important for Nuclear Import and Ribonucleoprotein Activity

  2019cites this paper
• Sumoylated α-synuclein translocates into the nucleus by karyopherin α6

  2018cites this paper
• Importin α1 is required for nuclear import of herpes simplex virus proteins and capsid assembly in fibroblasts and neurons

  2018cites this paper
• Oscillatory IL-2 stimulus reveals pertinent signaling timescales of T cell responsiveness

  2018cites this paper
• Importin α: functions as a nuclear transport factor and beyond

  2018cites this paper
• A set of enhanced green fluorescent protein concatemers for quantitative determination of nuclear localization signal strength.

  2017cites this paper
• Karyopherin Alpha Proteins Regulate Oligodendrocyte Differentiation

  2017cites this paper
• Identification of the nuclear localisation signal of O-GlcNAc transferase and its nuclear import regulation

  2016cites this paper
• Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7

  2016cites this paper
• Molecular changes in skin pigmented lesions of the coral trout Plectropomus leopardus.

  2016cites this paper
• Expression of importin-α isoforms in human nasal mucosa: implication for adaptation of avian influenza A viruses to human host

  2016cites this paper
• Karyopherin Alpha 1 Regulates Satellite Cell Proliferation and Survival by Modulating Nuclear Import

  2016cites this paper
• Karyopherin Alpha 2 Promotes the Inflammatory Response in Rat Pancreatic Acinar Cells Via Facilitating NF-κB Activation

  2016cites this paper
• Structure of Importin-α from a Filamentous Fungus in Complex with a Classical Nuclear Localization Signal

  2015cites this paper
• Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core Protein

  2015cites this paper
• Importin-α7 Is Involved in the Formation of Ebola Virus Inclusion Bodies but Is Not Essential for Pathogenicity in Mice.

  2015cites this paper
• Molecular characterization, tissue distribution, and immune reaction expression of karyopherins in the domestic silkworm (Bombyx mori).

  2015cites this paper
• The functional motifs for nucleocytoplasmic shuttling of C-terminal fragment of c-Met : A novel "pH-dependent' nuclear localization signal

  2015cites this paper
• Diversification of importin-α isoforms in cellular trafficking and disease states.

  2015cites this paper
• Importins and Exportins Regulating Allergic Immune Responses

  2014cites this paper
• Nuclear localization signals of varicella zoster virus ORF4

  2014cites this paper
• Recombinant Adeno-Associated Virus Utilizes Host Cell Nuclear Import Machinery To Enter the Nucleus

  2014cites this paper
• Understanding mechanisms of Adeno-associated virus vector transduction through pharmacological and biological approaches

  2014cites this paper
• Restricted expression of karyopherin alpha mRNA in the sea urchin suggests a role in neurogenesis.

  2014cites this paper
• Identification of Importin α 7 Specific Transport Cargoes Using a Proteomic Screening Approach*

  2014cites this paper
• A putative pH-dependent nuclear localization signal in the juxtamembrane region of c-Met

  2014cites this paper
• Exploration of Binary Virus–Host Interactions Using an Infectious Protein Complementation Assay*

  2013cites this paper
• Identification of Novel Stress Granule Components That Are Involved in Nuclear Transport

  2013cites this paper
• Hop-on hop-off: importin-α-guided tours to the nucleus in innate immune signaling

  2013cites this paper
• Domains of Lymphoid Enhancer Factor 1 and within the High-Mobility-Group DNA Binding Transport by Nuclear Localization Signals Recognition and Nuclear α Differential Importin-

  2013cites this paper
• Systemic Delivery of MicroRNA-181b Inhibits Nuclear Factor-&kgr;B Activation, Vascular Inflammation, and Atherosclerosis in Apolipoprotein E–Deficient Mice

  2013cites this paper
• UV-Induced Nuclear Import of XPA Is Mediated by Importin-α4 in An ATR-Dependent Manner

  2013cites this paper
• Adaptive mutations in the H5N1 polymerase complex.

  2013cites this paper
• Dynamic trafficking of STAT5 depends on an unconventional nuclear localization signal

  2013cites this paper
• KPNB1, XPO7 and IPO8 Mediate the Translocation ofNF‐κB/p65 into the Nucleus

  2013cites this paper
• Vitamin D and Immunomodulation in Bronchial Asthma

  2012cites this paper
• The Ins and Outs of HIV‐1 Tat

  2012cites this paper
• Biology and significance of the JAK/STAT signalling pathways

  2012cites this paper
• KPNA3 Variation Is Associated with Schizophrenia, Major Depression, Opiate Dependence and Alcohol Dependence

  2012cites this paper
• KPNA7, an oocyte- and embryo-specific karyopherin α subtype, is required for porcine embryo development.

  2012cites this paper
• Global enhancement of nuclear localization‐dependent nuclear transport in transformed cells

  2012cites this paper
• Changing subcellular localization of nuclear transport factors during human spermatogenesis.

  2012cites this paper
• Specific Cooperation Between Imp-α2 and Imp-β/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions

  2012cites this paper
• Differential use of importin-α isoforms governs cell tropism and host adaptation of influenza virus

  2011cites this paper
• Importin α7 Is Essential for Zygotic Genome Activation and Early Mouse Development

  2011cites this paper
• Karyopherins in nuclear transport of homeodomain proteins during development.

  2011cites this paper
• Induction of karyopherin α1 expression by indole-3-acetic acid in auxin-treated or overproducing tobacco plants

  2011cites this paper
• Molecular basis for specificity of nuclear import and prediction of nuclear localization.

  2011cites this paper
• Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery

  2011cites this paper
• Distinct roles for classical nuclear import receptors in the growth of multinucleated muscle cells.

  2011cites this paper
• The R(h)oads to Stat3: Stat3 activation by the Rho GTPases.

  2011cites this paper
• Regulation of nucleocytoplasmic transport in skeletal muscle.

  2011cites this paper
• Proteomic analysis of importin α-interacting proteins in adult mouse brain.

  2011cites this paper
• Live Cell Imaging Reveals Continuous STAT6 Nuclear Trafficking

  2010cites this paper
• Interaction of Sp1 zinc finger with transport factor in the nuclear localization of transcription factor Sp1.

  2010cites this paper
• Review of molecular mechanisms involved in the activation of the Nrf2-ARE signaling pathway by chemopreventive agents.

  2010cites this paper
• Stress-mediated nuclear stabilization of p53 is regulated by ubiquitination and importin-α3 binding

  2010cites this paper
• Studying the Nuclear Import of Thyroid Hormone Receptor Alpha using a Mammalian Two-Hybrid System

  2010cites this paper
• Common importin alpha specificity for papillomavirus E2 proteins.

  2010cites this paper
• The mechanisms regulating the subcellular localization of AID

  2010cites this paper
• Probing the Specificity of Binding to the Major Nuclear Localization Sequence-binding Site of Importin-α Using Oriented Peptide Library Screening*

  2010cites this paper
• Karyopherin α7 (KPNA7), a divergent member of the importin α family of nuclear import receptors

  2010cites this paper
• Importin α3 Interacts with HIV-1 Integrase and Contributes to HIV-1 Nuclear Import and Replication

  2010cites this paper
• Seizures increase importin‐β1 expression in NG2+ cells in the rat hippocampus

  2009cites this paper
• Identification of Essential Sequences for Cellular Localization in BRMS1 Metastasis Suppressor

  2009cites this paper
• Mechanisms and Signals for the Nuclear Import of Proteins

  2009cites this paper
• Identification of a selective nuclear import signal in adenosine deaminases acting on RNA

  2009cites this paper
• Nuclear translocation contributes to regulation of DNA excision repair activities.

  2009cites this paper
• Role of Importin Alpha8, a New Member of the Importin Alpha Family of Nuclear Transport Proteins, in Early Embryonic Development in Cattle1

  2009cites this paper
• Quantifying the possible cross-reactivity risk of an HPV16 vaccine.

  2009cites this paper
• Evasion of Interferon Responses by Ebola and Marburg Viruses

  2009cites this paper
• Evolution of the Metazoan-Specific Importin α Gene Family

  2009cites this paper
• The role of the nuclear transport system in cell differentiation.

  2009cites this paper
• Novel expression of importin alpha homologue in marine teleost, Pagrus major.

  2008cites this paper
• Drosophila Importin α1 Performs Paralog-Specific Functions Essential For Gametogenesis

  2008cites this paper
• IMPa-4, an Arabidopsis Importin α Isoform, Is Preferentially Involved in Agrobacterium-Mediated Plant Transformation[W]

  2008cites this paper
• NF-kappaB p52, RelB and c-Rel are transported into the nucleus via a subset of importin alpha molecules.

  2008cites this paper
• Daxx contains two nuclear localization signals and interacts with importin α3

  2008cites this paper