Purification of bromelain from pineapple wastes by ethanol precipitation

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Separation and Purification Technology

• Publication date

  2012-09-19

• Fields of study

  Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/J.SEPPUR.2012.06.042
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Thermal stability of free bromelain and bromelain-polyphenol complex in pineapple juice

  2011cited by this paper
• A review of the use of bromelain in cardiovascular diseases.

  2011cited by this paper
• Industrial scale natural products extraction.

  2011cited by this paper
• Ethanol precipitation and ultrafiltration of inulinases from Kluyveromyces marxianus

  2011cited by this paper
• Chemical modification of stem bromelain with anhydride groups to enhance its stability and catalytic activity

  2010cited by this paper
• Bromelain's activity and potential as an anti-cancer agent: Current evidence and perspectives.

  2010cited by this paper
• Purification and drying of bromelain

  2009cited by this paper
• Safety & efficacy of bromelain extract in debriding deep burns: Recent summary of all available data

  2009cited by this paper
• Bromelain inhibits COX-2 expression by blocking the activation of MAPK regulated NF-kappa B against skin tumor-initiation triggering mitochondrial death pathway.

  2009cited by this paper
• Examples of Structure-Function Relationships in Enzymatic Systems

  2009cited by this paper
• Use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes.

  2008cited by this paper
• Purification and characterization of transglutaminase from a newly isolated Streptomyces hygroscopicus

  2007cited by this paper
• Fourier transform infrared spectroscopic analysis of protein secondary structures.

  2007cited by this paper
• Purification and properties of a heat-stable exoinulinase isoform from Aspergillus fumigatus.

  2006cited by this paper
• Purificação de produtos biotecnológicos

  2005cited by this paper
• Therapeutic use, efficiency and safety of the proteolytic pineapple enzyme Bromelain-POS in children with acute sinusitis in Germany.

  2005cited by this paper
• Treatment with oral bromelain decreases colonic inflammation in the IL-10-deficient murine model of inflammatory bowel disease.

  2005cited by this paper
• Investigation of the stabilisation of freeze-dried lysozyme and the physical properties of the formulations.

  2004cited by this paper
• Exposure-response relationships and drug interactions of sirolimus

  2004cited by this paper
• Cryoprotection mechanisms of polyethylene glycols on lactate dehydrogenase during freeze-thawing

  2004cited by this paper
• Bromelain partitioning in two-phase aqueous systems containing PEO-PPO-PEO block copolymers.

  2004cited by this paper
• Isolation and purification of proteins

  2003cited by this paper
• Trifluoroethanol-induced "molten globule" state in stem bromelain.

  2003cited by this paper
• Bromelain treatment alters leukocyte expression of cell surface molecules involved in cellular adhesion and activation.

  2002cited by this paper
• Characterization of a partially folded intermediate of stem bromelain at low pH.

  2002cited by this paper
• Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations.

  2002cited by this paper
• Downstream processing of proteins : methods and protocols

  2000cited by this paper
• Xylanase and β-xylosidase separation by fractional precipitation

  1999cited by this paper
• Conformation, pH-induced conformational changes, and thermal unfolding of anti-p24 (HIV-1) monoclonal antibody CB4-1 and its Fab and Fc fragments.

  1999cited by this paper
• Artificial chaperone-assisted refolding of proteins.

  1998cited by this paper
• Cryopreservation of stallion spermatozoa.

  1996cited by this paper
• Circular dichroism of stem bromelain: a third spectral class within the family of cysteine proteinases.

  1994cited by this paper
• Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors.

  1993cited by this paper
• Secondary structure of the pentraxin female protein in water determined by infrared spectroscopy: effects of calcium and phosphorylcholine.

  1992cited by this paper
• Circular dichroism of cysteine proteinases from papaya latex. Evidence of differences in the folding of their polypeptide chains.

  1992cited by this paper
• Conformational studies on the N-linked carbohydrate chain of bromelain.

  1990cited by this paper
• The amino acid sequence of chymopapain from Carica papaya.

  1990cited by this paper
• Stem bromelain: Amino acid sequence and implications for weak binding of cystatin

  1989cited by this paper
• Examination of the secondary structure of proteins by deconvolved FTIR spectra

  1986cited by this paper
• Cloning and sequencing of papain-encoding cDNA.

  1986cited by this paper
• Complexes of rat alpha 1-macroglobulin and subtilisin are endocytosed by parenchymal liver cells.

  1985cited by this paper
• Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains.

  1985cited by this paper
• Structure of papain refined at 1.65 A resolution.

  1984cited by this paper
• Sensitivity of circular dichroism to protein tertiary structure class

  1983cited by this paper
• Complete structure of the carbohydrate moiety of stem bromelain. An application of the almond glycopeptidase for structural studies of glycopeptides.

  1979cited by this paper
• The amino acid sequence of the tryptic peptides from actinidin, a proteolytic enzyme from the fruit of Actinidia chinensis.

  1978cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Kinetics of the hydrolysis of N-benzoyl-L-serine methyl ester catalysed by bromelain and by papain. Analysis of modifier mechanisms by lattice nomography, computational methods of parameter evaluation for substrate-activated catalyses and consequences of postulated non-productive binding in bromelai

  1974cited by this paper
• Multiple molecular forms of stem bromelain. Isolation and characterization of two closely related components, SB1 and SB2.

  1973cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Stem bromelain—A new protease preparation from pineapple plants

  1957cited by this paper
• COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE

  1956cited by this paper
• CRYSTALLINE SOYBEAN TRYPSIN INHIBITOR

  1947cited by this paper

• Ultrasound‐assisted extraction and purification of bromelain from pineapple ( Ananas comosu s ) stem waste using ethanol precipitation and resin adsorption

  2025cites this paper
• A novel strategy for using ficin enzyme from fig leaves to extract collagen from tannery-trimming wastes.

  2025cites this paper
• Bioactive Bacterial Nanocellulose Membranes for Non-Surgical Debridement and Infection Prevention in Burn Wound Healing

  2025cites this paper
• Chlorella sp. as a promising protein source: insight to novel extraction techniques, nutritional and techno-functional attributes of derived proteins

  2025cites this paper
• Single step three phase partitioning for the purification of bromelain enzyme from pineapple (Ananas comosus) fruit

  2025cites this paper
• Exploration of a novel protease from Phytolacca dodecandra (Endod) berries for potential application in detergent formulation

  2024cites this paper
• Application of Immobilized β-Glucosidase from Candida boidinii in the Hydrolysis of Delignified Sugarcane Bagasse

  2024cites this paper
• Cysteine Proteases from Plants: Utilization in Foods and Nutraceuticals and Characterization Approaches for Quality Management

  2024cites this paper
• Profile of Bromelain Enzyme Extracts by Different Precipitation Methods and Effect of pH and Temperature on Protease Activity

  2024influential citation
• Optimization of enhanced collagen extraction from tannery rawhide trimming waste using pineapple peel-derived bromelain enzyme through response surface methodology

  2024cites this paper
• Production of a bacterial secretome highly efficient for the deconstruction of xylans

  2024cites this paper
• Composition and properties of ethanol-precipitated chickpea aquafaba

  2023cites this paper
• Valorization of pineapple rind for bromelain extraction using microwave assisted technique: optimization, purification, and structural characterization

  2023cites this paper
• Isolation of Proteolytic Enzyme from Pineapple Crown

  2023cites this paper
• A Vibrant Accelerating Strategic Solvent System for Sustainability and Functionality for Proteins: Unvarying Structure of Stem Bromelain in Hybrid Ionic Fluids

  2023cites this paper
• Anticancer properties of bromelain: State-of-the-art and recent trends

  2023cites this paper
• Sustainable eutectic mixture-based ultrasound assisted multifaceted valorisation of pineapple waste for bromelain and bioethanol production

  2022cites this paper
• Structure–process interaction in mass transfer processes: Application of ethanol and ultrasound in a vascular structure

  2022cites this paper
• Bromelain enzyme extraction from pineapple waste and its application as meat tenderizer

  2022cites this paper
• Comparative study of intermediate-wave and catalytic infrared drying on the kinetics and physicochemical properties of pineapple rings

  2022influential citation
• Multi-frequency power ultrasound as a novel approach improves intermediate-wave infrared drying process and quality attributes of pineapple slices

  2022influential citation
• A review on chemistry, therapeutic applications, extraction & purification of bromelain

  2022cites this paper
• Process Design and Techno-Economic Feasibility Analysis of an Integrated Pineapple Processing Waste Biorefinery

  2022cites this paper
• Low-cost approaches to producing and concentrating stable lipases and the evaluation of inductors

  2022cites this paper
• Beneficial Properties of Bromelain

  2021cites this paper
• Soluble Expression and Catalytic Properties of Codon-Optimized Recombinant Bromelain from MD2 Pineapple in Escherichia coli

  2021cites this paper
• In-flow SAXS investigation of whey protein isolate hydrolyzed by bromelain

  2021cites this paper
• Preparation and In Vitro Characterization of Gels Based on Bromelain, Whey and Quince Extract

  2021cites this paper
• Composición nutricional y compuestos fitoquímicos de la piña (Ananas comosus) y su potencial emergente para el desarrollo de alimentos funcionales.

  2021cites this paper
• Recent Advances and Insights into Bromelain Processing, Pharmacokinetics and Therapeutic Uses

  2021influential citation
• INCORPORATING PINEAPPLE PEEL EXTRACT INTO CHITOSAN COATING FOR RETAINING POSTHARVEST QUALITY OF WHITE SHRIMP (Litopaeneus vannamei)

  2021cites this paper
• Bromelain recovery from pineapple subproducts by ultrafiltration and aqueous biphasic systems: processes comparison and integration

  2021cites this paper
• Bromelain, a Group of Pineapple Proteolytic Complex Enzymes (Ananas comosus) and Their Possible Therapeutic and Clinical Effects. A Summary

  2021cites this paper
• Composition, enzyme and antioxidant activities of pineapple

  2021cites this paper
• Bromelain and Nisin: The Natural Antimicrobials with High Potential in Biomedicine

  2021cites this paper
• Puri cation of the recombinant green uorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography

  2021cites this paper
• Integrated Biorefinery Strategy for Valorization of Pineapple Processing Waste into High-Value Products

  2021cites this paper
• Conventional macro- and micromolecules separation

  2021cites this paper
• Pineapple

  2020cites this paper
• Papain grafted into the silica coated iron-based magnetic nanoparticles ‘IONPs@SiO2-PPN’ as a new delivery vehicle to the HeLa cells

  2020cites this paper
• Enzymatic recovery of silver from waste radiographic film: Optimize with response surface methodology

  2020cites this paper
• Optimization of membrane dispersion ethanol precipitation process with a set of temperature control improved equipment

  2020cites this paper
• Evaluation of dissolution of purified bromelain from pineapple core extract (Ananas comosus [L.] Merr) in the form of chitosan-coated microspheres

  2020cites this paper
• A review of clinical uses of Bromelain and concerned purification methods to obtain its pharmacological effects efficiently

  2020cites this paper
• Recovery of high value-added compounds from pineapple, melon, watermelon and pumpkin processing by-products: An overview.

  2020cites this paper
• Extraction of flavonoids from Cyclocarya paliurus (Juglandaceae) leaves using ethanol/salt aqueous two‐phase system coupled with ultrasonic

  2020cites this paper
• Effect of Maltodextrin Content and Inlet Temperature on the Powder Qualities of Spray-Dried Pineapple (Ananas comosus) Waste Extract

  2020cites this paper
• Bromelain nanoemulsion formulation resulting from partial purification of pineapple core (Ananas comosus [L.] Merr) and in vitro testing as antiinflammation

  2020cites this paper
• Pineapple

  2020cites this paper
• Inhibitor effect of PCMB and PMSF towards partially purified product from pineapple core (Ananas comosus [L.] Merr) using gel filtration column chromatography method

  2020cites this paper
• BROMELAIN SEPARATION AND PURIFICATION PROCESSES FROM PINEAPPLE EXTRACT

  2019influential citation
• Development of L-Asparaginase Biobetters: Current Research Status and Review of the Desirable Quality Profiles

  2019cites this paper
• Stabilizing bromelain for therapeutic applications by adsorption immobilization on spores of probiotic Bacillus.

  2019cites this paper
• Polysaccharides production from soybean curd residue via Morchella esculenta.

  2019cites this paper
• Integrated strategy for purification of esterase from Aureobasidium pullulans

  2019cites this paper
• Properties of bromelain extract from different parts of the pineapple variety Morris

  2019cites this paper
• The use of bromelain as a feed additive in fish diets: Growth performance, intestinal morphology, digestive enzyme and immune response of juvenile Sterlet (Acipenser ruthenus)

  2019cites this paper
• Selective purification of protease from ginger and sodom apple by ultrasound assisted liquid-liquid microextraction using natural deep eutectic solvent

  2019cites this paper
• Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain–Chitosan Nanoparticles

  2019cites this paper
• A high efficiency method combining metal chelate ionic liquid-based aqueous two-phase flotation with two-step precipitation process for bromelain purification.

  2019cites this paper
• An Overview of Proteases: Production, Downstream Processes and Industrial Applications

  2019influential citation
• Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography

  2019cites this paper
• Effect of Maltodextrin Content and Inlet Temperature on the Powder Qualities of Spray-Dried Pineapple (Ananas comosus) Waste Extract

  2019cites this paper
• In Vivo antiplatelet activity aggregation assay of bromelain fractionate by ethanol from extract pineapple core (Ananas comosus [l.] merr.)

  2018cites this paper
• Integrated strategy for puri fi cation of esterase from Aureobasidium pullulans Ailton

  2018cites this paper
• Influence of Different Solvents in the Recovery and Pre-Purification of Pectinases from Aspergillus niger

  2018cites this paper
• Effect of maltodextrin concentration and slurry temperature on pineapple powder using ultrasonic spray dryer

  2018cites this paper
• Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems

  2018cites this paper
• Characterization of PNIPAAm-co-AAm hydrogels for modified release of bromelain

  2018cites this paper
• Extraction and identification of endopeptidases in convection dried papaya and pineapple residues: A methodological approach for application to higher scale.

  2018cites this paper
• Fabrication of a novel magnetic mesoporous molecularly imprinted polymer based on pericarpium granati-derived carrier for selective absorption of bromelain.

  2018cites this paper
• Hidrogéis de pnipaam-co-aam como sistemas carreadores de produtos bioativos de interesse farmacêutico : Pnipaam-co-aam hydrogels as carrier systems of bioactive products of pharmaceutical interest

  2018cites this paper
• Preservation of the antibacterial activity of enzymes against Alicyclobacillus spp. through microencapsulation

  2018cites this paper
• Isolation and characterization of bromelain enzyme from pineapple and its utilization as anti-browning agent

  2017influential citation
• Bromelain: from production to commercialisation.

  2017cites this paper
• Comparative study of two methods of fractionation bromelain from pineapple core extract (Ananas comosus)

  2017cites this paper
• Utilization of Waste from Tropical Fruits

  2017cites this paper
• The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells

  2017cites this paper
• Utilisation d'agents chimiques pour l'amélioration des phases post-fécondation chez la perche européenne Perca fluviatilis

  2017cites this paper
• Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1

  2017cites this paper
• Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan

  2017cites this paper
• Plant By-Products and Food Industry Waste: A Source of Nutraceuticals and Biopolymers

  2017cites this paper
• Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols.

  2017cites this paper
• Single step purification of bromelain from Ananas comosus pulp using a polymer/salt aqueous biphasic system

  2017cites this paper
• Comparison of purification methods to purify recombinant bromelain from Escherichia coli BL21-A1 = Perbandingan kaedah penulenan rekombinan bromelain daripada Escherichia coli BL21-A1

  2017cites this paper
• Bromelain-Functionalized Multiple-Wall Lipid-Core Nanocapsules: Formulation, Chemical Structure and Antiproliferative Effect Against Human Breast Cancer Cells (MCF-7)

  2016cites this paper
• Changes in the physicochemical quality, functional properties, and actinidin content of kiwifruit (Actinidia chinensis) during postharvest storage

  2016cites this paper
• Value added processing and utilization of pineapple by‐products

  2016cites this paper
• β-cyclodextrin assisted two-stage foam fractionation of bromelain from the crude extract of pineapple peels

  2016cites this paper
• Identification and Functional Characterization of Cysteine Protease from Nine Pear Cultivars (Pyrus pyrifolia)

  2016cites this paper
• Comparison of four purification methods to purify cysteine protease from Asian pear fruit (Pyrus pyrifolia)

  2016cites this paper
• Functional properties of pineapple

  2016cites this paper
• Comparative Study on Characterization of Bromelain Extracted from the Stem and Fruit of Pineapple (Ananas comosus)

  2016cites this paper
• Functional characterization of purified pear protease and its proteolytic activities with casein and myofibrillar proteins

  2016cites this paper
• Bromelain: Methods of Extraction, Purification and Therapeutic Applications

  2016influential citation
• Stability, purification, and applications of bromelain: A review

  2016influential citation
• Characteristic properties of crude pineapple waste extract for bromelain purification by membrane processing

  2015influential citation
• Comparative Study on The Extraction and Purification The Stem and Fruit Bromelain From Pineapple (Ananas comosus)

  2015influential citation
• Purification of pectinases from Aspergillus niger ATCC 9642 by ethanol precipitation

  2015cites this paper
• Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus

  2015cites this paper