New Roles for Conserved Regions within a ς54-dependent Enhancer-binding Protein*

23 amino acid substitutions were made in the C7 and C3 regions of pspFΔHTH, a protein required to convert ς54 closed promoter complexes to open complexes. These mutants were assayed for transcriptional competence, for the ability to hydrolyze ATP, for their multimerization state, and for their ability to interact with ς54 and its holoenzyme. C7 region mutants caused the protein to assume a compact form. This property could be mimicked by the addition of ATP, implying that compaction via C7 and ATP is part of the activation process. A number of C3 mutants were important for energy coupling, as indicated previously for several members of this activator family (1, 2). However, a patch within C3 influenced oligomerization. The C3 region was especially important in interacting with ς54 during the transition state but not important in inducing ς54holoenzyme to engage the nontemplate strand of the promoter. It is proposed that both regions contain deterrent functions that prevent premature activation. Overall, the results imply unexpected roles for the C7 and C3 regions of this protein family during promoter activation.

• Publication year

  2002

• Venue

  Journal of Biological Chemistry

• Publication date

  2002-11-01

• Fields of study

  Biology

• Identifiers
  DOI 10.1074/jbc.M206912200
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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