The birch GLK1 transcription factor mutant reveals new insights in chlorophyll biosynthesis and chloroplast development.

Birch (Betula platyphylla × B. pendula) is a precious material for landscaping due to its attractive white bark and straight trunk. In this study, we characterized a T-DNA caused yellow-green leaf mutant yl in birch. We identified six insertion sites (ISs) in yl mutant by genome resequencing and found a 40-kb deletion containing BpGLK1 around IS2 on chromosome 2. Complementation experiment of yl mutant and repression of BpGLK1 in wild type birch confirmed that BpGLK1 gene was responsible for the mutated phenotype of yl. Physiological and ultrastructural analysis showed that the leaves of yl mutant and BpGLK1 repression lines had decreased chlorophyll content and defective chloroplast development compared to the wild type. Furthermore, the loss function of BpGLK1 also affected photosynthesis in leaves. Transcriptomics, proteomics and ChIP-PCR results revealed that BpGLK1 directly interacted with the promoter of genes related to antenna protein, chlorophyll biosynthesis, photosystem subunit synthesis and regulated their expression. Collectively, our research not only provided a new insight into the mechanism of chloroplast development and chlorophyll biosynthesis regulated by BpGLK1, but also provided new transgenic birch varieties with various levels of yellowing leaves by repressing BpGLK1 gene expression.

• Publication year

  2019

• Venue

  Journal of Experimental Botany

• Publication date

  2019-06-28

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1093/jxb/erz128PMID 30921458
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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