Thrombin‐induced proliferation and expression of platelet‐derived growth factor‐A chain gene in human vascular smooth muscle cells

Treatment of human vascular smooth muscle cells (SMC) with human α‐thrombin greatly increased DNA synthesis and cell proliferation. Both the integrity of the catalytic site and that of the anion binding exosite were required for expression of this activity. Experiments employing Northerns indicated induction of c‐ƒos expression as well as a time‐dependent induction of platelet‐derived growth factor‐A (PDGF‐A) gene by thrombin. The thrombin mitogenic activity was potentiated by PDGF‐BB, insulin and the vasoconstrictor peptide endothelin‐1 suggesting synergism by convergence of intracellular growth‐promoting signals. SMC treatment with pertussis toxin and forskolin indicated that the mitogenic activity of thrombin may be induced via signal transduction mechanism(s) involving changes in cAMP levels and activation of a Gi‐like protein. These results suggest that thrombin may play a functional role in the regulation of human vascular SMC proliferation.

• Publication year

  1992

• Venue

  FEBS Letters

• Publication date

  1992-12-14

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0014-5793(92)80961-FPMID 1333990
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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