Xenopus borealis as an alternative source of oocytes for biophysical and pharmacological studies of neuronal ion channels

For the past 30 years, oocytes from Xenopus laevis have been extensively used to express and characterise ion channels in an easily controlled environment. Here we report the first use of oocytes from the closely related species Xenopus borealis as an alternative expression system for neuronal ion channels. Using the two-electrode voltage-clamp technique, we show that a wide variety of voltage- and ligand-gated ion channels have the same channel properties and pharmacological profiles when expressed in either X. laevis or X. borealis oocytes. Potential advantages of the X. borealis oocytes include a smaller endogenous chloride current and the ability to produce more intense fluorescence signals when studied with voltage-clamp fluorometry. Scanning electron microscopy revealed a difference in vitelline membrane structure between the two species, which may be related to the discrepancy in fluorescence signals observed. We demonstrate that X. borealis oocytes are a viable heterologous system for expression of neuronal ion channels with some potential advantages over X. laevis oocytes for certain applications.

• Publication year

  2015

• Venue

  Scientific Reports

• Publication date

  2015-10-06

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/srep14763PMID 26440210PMCID 4594035
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Scanning electron microscopy identified a difference in vitelline membrane structure between the two Xenopus species, which may be related to the fluorescence signal discrepancy.

  Confidence 0.86

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• Xenopus borealis oocytes produce more intense fluorescence signals in voltage-clamp fluorometry than Xenopus laevis oocytes.

  Confidence 0.91

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• Xenopus borealis oocytes have a smaller endogenous chloride current than Xenopus laevis oocytes.

  Confidence 0.93

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• Using the two-electrode voltage-clamp technique, a wide variety of voltage-gated ion channels and ligand-gated ion channels show the same channel properties and pharmacological profiles in Xenopus laevis oocytes and Xenopus borealis oocytes.

  Confidence 0.97

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review

• endogenous chloride current

  electrical current

  The native chloride current generated by the oocyte itself rather than by an introduced channel.

  Aliases: native chloride current

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• ligand-gated ion channels

  protein class

  Ion channels that open or close in response to binding of a chemical ligand.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• neuronal ion channels

  protein class

  Ion channels associated with neuronal function that were expressed and characterized in the oocytes.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• two-electrode voltage-clamp technique

  method

  An electrophysiological recording method used to measure ion channel activity in oocytes.

  Aliases: TEVC, two-electrode voltage clamp

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• vitelline membrane structure

  anatomical structure

  The structural organization of the oocyte vitelline membrane examined by scanning electron microscopy.

  Aliases: vitelline membrane

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• voltage-clamp fluorometry

  method

  A method that combines voltage-clamp recording with fluorescence readout from labeled proteins or membranes.

  Aliases: VCF

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• voltage-gated ion channels

  protein class

  Ion channels whose opening is controlled by changes in membrane voltage.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• xenopus borealis oocytes

  experimental system, material

  Oocytes from the frog species Xenopus borealis used here as a heterologous expression host.

  Aliases: borealis oocytes, X. borealis oocytes

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review
• xenopus laevis oocytes

  experimental system, material

  Oocytes from the frog species Xenopus laevis used as the comparison expression host in the study.

  Aliases: laevis oocytes, X. laevis oocytes

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewAK (4715169a40) review

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