Use ofXenopus oocytes for the functional expression of plasma membrane proteins

No abstract is available for this paper.

• Publication year

  1990

• Venue

  Journal of Membrane Biology

• Publication date

  1990-09-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1007/BF01868451PMID 2231695
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Sodium-alanine cotransport in oocytes ofXenopus laevis: Correlation of alanine and sodium fluxes with potential and current changes

  2005cited by this paper
• Voltage dependence of the rheogenic Na+/K+ ATPase in the membrane of oocytes ofXenopus laevis

  2005cited by this paper
• Potential dependence of the “electrically silent” anion exchange across the plasma membrane ofXenopus oocytes mediated by the band-3 protein of mouse red blood cells

  2005cited by this paper
• [Functional expression of plasma membrane proteins in the Xenopus laevis oocyte system].

  1994cited by this paper
• Functional expression and sites of gene transcription of a novel alpha subunit of the GABAA receptor in rat brain.

  1990cited by this paper
• Functional expression and sites of gene transcription of a novel α subunit of the GABAA receptor in rat brain

  1990cited by this paper
• Study of ATP binding in the active site of Na+,K+‐ATPase as probed by ultraviolet resonance Raman spectroscopy

  1990cited by this paper
• Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides.

  1989cited by this paper
• Importance of a novel GABAA receptor subunit for benzodiazepine pharmacology

  1989cited by this paper
• Pharmacological characterization and region-specific expression in brain of the beta 2- and beta 3-subunits of the rat GABAA receptor.

  1989cited by this paper
• Use of chemical modifications and site-directed mutagenesis to probe the functional role of thiol groups on the gamma subunit of Torpedo californica acetylcholine receptor.

  1989cited by this paper
• Coupling of exogenous receptors to phospholipase C in Xenopus oocytes through pertussis toxin-sensitive and -insensitive pathways. Cross-talk through heterotrimeric G-proteins.

  1989cited by this paper
• A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning

  1989cited by this paper
• Tissue-specific processing and polarized compartmentalization of clone-produced cholinesterase in microinjectedXenopus oocytes

  1989cited by this paper
• A novel calcium‐dependent chloride current in Xenopus oocytes injected with brain messenger RNA.

  1989cited by this paper
• Properties of ShB A-type potassium channels expressed in Shaker mutant Drosophila by germline transformation.

  1989cited by this paper
• Anion transport in oocytes of Xenopus laevis induced by expression of mouse erythroid band 3 protein‐‐encoding cRNA and of a cRNA derivative obtained by site‐directed mutagenesis at the stilbene disulfonate binding site.

  1989cited by this paper
• Assembly and N-glycosylation of all ACh receptor subunits are required for their efficient insertion into plasma membranes.

  1989cited by this paper
• Effects of antidepressants on serotonin-evoked current in Xenopus oocytes injected with rat brain mRNA.

  1989cited by this paper
• Sequence and expression of a novel GABAA receptor alpha subunit.

  1989cited by this paper
• Pharmacological characterization and region‐specific expression in brain of the β2‐ and β3‐subunits of the rat GABAA receptor

  1989cited by this paper
• Formation of hybrid cell-cell channels.

  1989cited by this paper
• Protein kinase C modulates neurotransmitter responses in Xenopus oocytes injected with rat brain RNA.

  1989cited by this paper
• Sequence and expression of a novel GABAA receptor α subunit

  1989cited by this paper
• Molecular cloning of the thyrotropin receptor.

  1989cited by this paper
• The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: β4

  1989cited by this paper
• Gating of single Shaker potassium channels in Drosophila muscle and in Xenopus oocytes injected with Shaker mRNA.

  1989cited by this paper
• Glutamate receptor subtypes may be classified into two major categories: a study on Xenopus oocytes injected with rat brain mRNA.

  1989cited by this paper
• Expression of the thyroid sodium/iodide symporter in Xenopus laevis oocytes.

  1989cited by this paper
• Functional expression of the human HepG2 and rat adipocyte glucose transporters in Xenopus oocytes. Comparison of kinetic parameters.

  1989cited by this paper
• Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA.

  1989cited by this paper
• Inward rectifier produced by Xenopus oocytes injected with mRNA extracted from carp olfactory epithelium

  1989cited by this paper
• Single-channel currents of rat neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes.

  1989cited by this paper
• Expression of active alpha-3 subunit of rat brain Na+,K+-ATPase from the messenger RNA injected into Xenopus oocytes.

  1989cited by this paper
• mRNA from NCB-20 cells encodes the N-methyl-D-aspartate/phencyclidine receptor: a Xenopus oocyte expression study.

  1989cited by this paper
• Evidence for the existence of a cardiac specific isoform of the α1 subunit of the voltage dependent calcium channel

  1989cited by this paper
• Expression of amiloride-blockable sodium channels in Xenopus oocytes.

  1989cited by this paper
• GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs.

  1989cited by this paper
• Expression of a cloned rat brain potassium channel in Xenopus oocytes.

  1989cited by this paper
• The major kidney band 3 gene transcript predicts an amino-terminal truncated band 3 polypeptide.

  1989cited by this paper
• Modulation of the voltage-dependent sodium channel by agents affecting G-proteins: a study in Xenopus oocytes injected with brain RNA.

  1989cited by this paper
• Functional expression in Xenopus oocytes of the strychnine binding 48 kd subunit of the glycine receptor.

  1989cited by this paper
• Molecular characterization of a functional cDNA for rat substance P receptor.

  1989cited by this paper
• Cloning and expression of a Xenopus embryonic gap junction protein.

  1989cited by this paper
• A novel α subunit in rat brain GABAA receptors

  1989cited by this paper
• The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: beta 4.

  1989cited by this paper
• Change in desensitization of cat muscle acetylcholine receptor caused by coexpression of Torpedo acetylcholine receptor subunits in Xenopus oocytes.

  1989cited by this paper
• Specific block of calcium channel expression by a fragment of dihydropyridine receptor cDNA.

  1989cited by this paper
• N-methyl-D-aspartate/glycine and quisqualate/kainate receptors expressed in Xenopus oocytes: antagonist pharmacology.

  1989cited by this paper
• The rat β1 ‐subunit of the GABAA receptor forms a picrotoxin‐sensitive anion channel open in the absence of GABA

  1989cited by this paper
• Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel

  1989cited by this paper
• Activation of glucose uptake by insulin and insulin-like growth factor I in Xenopus oocytes.

  1989cited by this paper
• Cloning and expression of a novel rat GABAA receptor

  1989cited by this paper
• Cloning by functional expression of a member of the glutamate receptor family

  1989cited by this paper
• Sodium channels from human brain RNA expressed in Xenopus oocytes. Basic electrophysiologic characteristics and their modification by diphenylhydantoin.

  1989cited by this paper
• Molecular basis of functional diversity of voltage‐gated potassium channels in mammalian brain.

  1989cited by this paper
• A novel alpha subunit in rat brain GABAA receptors.

  1989cited by this paper
• Characterization of a Na+/glucose cotransporter cloned from rabbit small intestine

  1989cited by this paper
• Formation of gap junctions by expression of connexins in Xenopus oocyte pairs.

  1989cited by this paper
• A role for the beta-subunit in the expression of functional Na+-K+-ATPase in Xenopus oocytes.

  1989cited by this paper
• N-methyl-D-aspartate activates different channels than do kainate and quisqualate.

  1989cited by this paper
• The tremorigen aflatrem is a positive allosteric modulator of the gamma-aminobutyric acidA receptor channel expressed in Xenopus oocytes.

  1989cited by this paper
• Selective expression of an amiloride-inhibitable Na+ conductance from mRNA of respiratory epithelium in Xenopus laevis oocytes.

  1989cited by this paper
• Mutant potassium channels with altered binding of charybdotoxin, a pore-blocking peptide inhibitor.

  1989cited by this paper
• Structural requirements for activation of the glycine coagonist site of N-methyl-D-aspartate receptors expressed in Xenopus oocytes.

  1989cited by this paper
• Molecular cloning and sequence analysis of human genomic DNA encoding a novel membrane protein which exhibits a slowly activating potassium channel activity.

  1989cited by this paper
• Identification of a novel gene encoding an insulin-responsive glucose transporter protein.

  1989cited by this paper
• Endogenousd-glucose transport in oocytes ofXenopus laevis

  1989cited by this paper
• Concanavalin A potentiates NMDA-evoked responses in the Xenopus oocyte expression system.

  1989cited by this paper
• Mercuric ions are potent noncompetitive antagonists of human brain kainate receptors expressed in Xenopus oocytes.

  1989cited by this paper
• Structural parts involved in activation and inactivation of the sodium channel

  1989cited by this paper
• Functional expression of the amiloride-sensitive sodium channel in Xenopus oocytes.

  1989cited by this paper
• Expression of the mammalian Na+-independent L system amino acid transporter in Xenopus laevis oocytes.

  1989cited by this paper
• A specific quisqualate agonist inhibits kainate responses induced in Xenopus oocytes injected with rat brain RNA.

  1989cited by this paper
• Expression of Ba currents in Xenopus oocyte injected with pregnant rat myometrium mRNA

  1989cited by this paper
• Primary structure and functional expression of a mammalian skeletal muscle sodium channel.

  1989cited by this paper
• Expression of neurotransmitter transport from rat brain mRNA in Xenopus laevis oocytes.

  1988cited by this paper
• Evidence for the involvement of more than one mRNA species in controlling the inactivation process of rat and rabbit brain Na channels expressed in Xenopus oocytes

  1988cited by this paper
• Expression of ACh-activated channels and sodium channels by messenger RNAs from innervated and denervated muscle

  1988cited by this paper
• Ouabain sensitivity of a chimeric alpha subunit (Torpedo/rat) of the (Na,K)ATPase expressed in Xenopus oocyte.

  1988cited by this paper
• Repression of nicotinic acetylcholine receptor expression by antisense RNAs and an oligonucleotide.

  1988cited by this paper
• Efficient processing and expression of human nerve growth factor receptors in Xenopus laevis oocytes: effects on maturation

  1988cited by this paper
• Hemispheric asymmetry of rapid chloride responses to inositol trisphosphate and calcium in Xenopus oocytes

  1988cited by this paper
• Expression of a bovine rhodopsin gene in Xenopus oocytes: demonstration of light-dependent ionic currents.

  1988cited by this paper
• Four cDNA clones from the Shaker locus of Drosophila induce kinetically distinct A-type potassium currents in Xenopus oocytes.

  1988cited by this paper
• Activation of ionic currents in Xenopus oocytes by corticotropin-releasing peptides.

  1988cited by this paper
• Forskolin alters acetylcholine receptor gating by a mechanism independent of adenylate cyclase activation.

  1988cited by this paper
• Expression of the murine interferon gamma receptor in Xenopus laevis oocytes.

  1988cited by this paper
• Expression of functional potassium channels from Shaker cDNA in Xenopus oocytes

  1988cited by this paper
• Structural basis of beta-adrenergic receptor subtype specificity studied with chimeric beta 1/beta 2-adrenergic receptors.

  1988cited by this paper
• Structure and function of voltage-sensitive ion channels.

  1988cited by this paper
• Expression of GABA and glycine receptors by messenger RNAs from the developing rat cerebral cortex

  1988cited by this paper
• A rat brain Na+ channel alpha subunit with novel gating properties.

  1988cited by this paper
• Differences in receptor-evoked membrane electrical responses in native and mRNA-injected Xenopus oocytes.

  1988cited by this paper
• Potassium channels expressed from rat brain cDNA have delayed rectifier properties

  1988cited by this paper
• Atrial natriuretic peptide clearance receptor. Complete sequence and functional expression of cDNA clones.

  1988cited by this paper
• mRNA-induced expression of the cardiac Na+-Ca2+ exchanger in Xenopus oocytes.

  1988cited by this paper
• Allosteric modulation by benzodiazepine receptor ligands of the GABAA receptor channel expressed in Xenopus oocytes

  1988cited by this paper
• Biophysical and pharmacological properties of cloned GABAA receptor subunits expressed in Xenopus oocytes.

  1988cited by this paper
• Calcium mobilization by angiotensin II and neurotransmitter receptors expressed in Xenopus laevis oocytes

  1988cited by this paper

• P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes.

  2020cites this paper
• Disease-associated HCN4 V759I variant is not sufficient to impair cardiac pacemaking

  2020cites this paper
• 3D Biofabrication Using Living Cells for Applications in Biohybrid Sensors and Actuators.

  2020cites this paper
• Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

  2020cites this paper
• Analysis of membrane transport mechanisms of endogenous substrates using chromatographic techniques.

  2019cites this paper
• Functional and structural analysis of rare SLC2A2 variants associated with Fanconi‐Bickel syndrome and metabolic traits

  2019cites this paper
• Current Research Method in Transporter Study.

  2019cites this paper
• The effects of Capsaicin on the function of human α7 Nicotine Acetylcholine receptors expressed in Xenopus Oocytes.

  2018cites this paper
• A Xenopus oocyte model system to study action potentials

  2018cites this paper
• Expression cloning human and rat renal cortex Na/Pi cotransporters: behind the scenes in the Murer laboratory

  2018cites this paper
• Gating control and K+ uptake by the KAT1 K+ channel leaveraged through membrane anchoring of the trafficking protein SYP121

  2018cites this paper
• Modulation of DEG/ENaCs by Amphiphiles Suggests Sensitivity to Membrane Alterations.

  2018cites this paper
• Membrane protein-based biosensors

  2018cites this paper
• Functional characterization of anion channels of the SLAC/SLAH family in Arabidopsis thaliana

  2017cites this paper
• Molecular insights into the normal operation , regulation and multisystemic roles of K +-Cl − cotransporter 3 ( KCC 3 )

  2017cites this paper
• Uptake Assays in Xenopus laevis Oocytes Using Liquid Chromatography-mass Spectrometry to Detect Transport Activity.

  2017cites this paper
• Transmembrane solute transport in the apicomplexan parasite Plasmodium.

  2017cites this paper
• Potential new drug targets and therapeutic approaches for parasitic nematode infections

  2017cites this paper
• Molecular insights into the normal operation, regulation, and multisystemic roles of K+-Cl- cotransporter 3 (KCC3).

  2017cites this paper
• Combining Cytotoxicity Assessment and Xenopus laevis Phenotypic Abnormality Assay as a Predictor of Nanomaterial Safety

  2017cites this paper
• A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes.

  2016cites this paper
• Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments.

  2016cites this paper
• Xenopus borealis as an alternative source of oocytes for biophysical and pharmacological studies of neuronal ion channels

  2015cites this paper
• Pre‐Plated Cell Lines for ADMETox Applications in the Pharmaceutical Industry

  2015cites this paper
• Natural Products as Scaffolds for the Development of GABAA Receptor Ligands

  2015cites this paper
• Xenopus Oocytes Isolation and Microinjection

  2014cites this paper
• Transport assays in filamentous fungi: kinetic characterization of the UapC purine transporter of Aspergillus nidulans.

  2014cites this paper
• Drug-transporter interaction testing in drug discovery and development

  2013cites this paper
• The Xenopus oocyte: a single-cell model for studying Ca2+ signaling.

  2013cites this paper
• Characterization of choline uptake in Trypanosoma brucei procyclic and bloodstream forms.

  2013cites this paper
• Housing and husbandry of Xenopus laevis affect the quality of oocytes for heterologous expression studies.

  2011cites this paper
• Identification and Characterization of Cellular Manganese Efflux Mechanisms

  2011cites this paper
• Dengue virus PrM/M proteins fail to show pH-dependent ion channel activity in Xenopus oocytes.

  2011cites this paper
• Inhibition of G Protein-Activated Inwardly Rectifying K+ Channels by Different Classes of Antidepressants

  2011cites this paper
• Microinjection into Xenopus Oocytes

  2010cites this paper
• Essential and Toxic Metal Transport in the Liver

  2010cites this paper
• Endogenous transport systems in the Xenopus laevis oocyte plasma membrane.

  2010cites this paper
• Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors

  2010cites this paper
• Expression cloning and radiotracer uptakes in Xenopus laevis oocytes

  2008cites this paper
• Evaluation of drug-transporter interactions using in vitro and in vivo models.

  2007cites this paper
• Untersuchungen zur Pathophysiologie der Cystinurie Typ A: Elektrophysiologische Charakterisierung des hrBAT Wildtyps und der Mutation T216M in Xenopus Oozyten

  2006cites this paper
• Elektrophysiologische Untersuchungen an Aminosäuretransporter des Systems SN1

  2005cites this paper
• The Xenopus oocyte: system for the study of functional expression and modulation of proteins.

  2005cites this paper
• A naturally occurring human Nedd4-2 variant displays impaired ENaC regulation in Xenopus laevis oocytes.

  2004cites this paper
• Transportador de aminoácidos heteromérico xCT Identificación, caracterización funcional y topología

  2004cites this paper
• Effects of organometal(loid) compounds on neuronal ion channels: possible sites for neurotoxicity

  2004cites this paper
• Na+/amino acid coupling stoichiometry of rheogenic system B0,+ transport in Xenopus oocytes is variable

  2004cites this paper
• Bufo marinus Oocytes as a Model for Ion Channel Protein Expression and Functional Characterization for Electrophysiological Studies

  2004cites this paper
• Functional expression and cellular distribution of diastrophic dysplasia sulfate transporter (DTDST) gene mutations in HEK cells.

  2004cites this paper
• Electrochemical monitoring of transport by a vesicular monoamine transporter expressed in Xenopus oocytes.

  2004cites this paper
• Characteristics of ecto-ATPase of Xenopus oocytes and the inhibitory actions of suramin on ATP breakdown

  2004cites this paper
• Determination of native oligomeric state and substrate specificity of rat NTPDase1 and NTPDase2 after heterologous expression in Xenopus oocytes.

  2003cites this paper
• Characterization of urea transport in Bufo arenarum oocytes.

  2003cites this paper
• Stromal cell-derived receptor 2 and cytochrome b561 are functional ferric reductases.

  2003cites this paper
• Defining the molecular and cellular basis of toxicity using comparative models.

  2002cites this paper
• Xenopus helveticus, an endangered species?

  2002cites this paper
• Expression of functional neurotransmitter receptors in Xenopus oocytes after injection of human brain membranes

  2002cites this paper
• Methods used to study intestinal nutrient transport: past and present.

  2002cites this paper
• Transport proteins of Plasmodium falciparum: defining the limits of metabolism.

  2001cites this paper
• Cysteine string proteins are associated with cortical granules of Xenopus laevis oocytes

  2001cites this paper
• Physiological roles and regulation of mammalian sulfate transporters.

  2001cites this paper
• Mutations in the diastrophic dysplasia sulfate transporter (DTDST) gene: correlation between sulfate transport activity and chondrodysplasia phenotype.

  2001cites this paper
• Expression of the Human Menkes ATPase in Xenopus laevis Oocytes

  2001cites this paper
• Expression cloning of two genes that together mediate organic solute and steroid transport in the liver of a marine vertebrate

  2001cites this paper
• Cloning and characterization of an arginine vasotocin receptor from the euryhaline flounder Platichthys flesus.

  2001cites this paper
• Expression and functional characterization of the mt1 melatonin receptor from rat brain in Xenopus oocytes: evidence for coupling to the phosphoinositol pathway

  2001cites this paper
• An endogenous monocarboxylate transport in Xenopus laevis oocytes.

  2000cites this paper
• Xenopus oocytes as an expression system for plant transporters.

  2000cites this paper
• Functional characterization of the human high‐affinity choline transporter 1

  2000cites this paper
• Ion currents of Xenopus laevis oocytes: state of the art.

  1999cites this paper
• Effects of 2-phenoxyethanol on N-methyl-d-aspartate (NMDA) receptor-mediated ion currents

  1999cites this paper
• Interactions of snake dendrotoxins with potassium channels.

  1999cites this paper
• Expression of parasite transporters in Xenopus oocytes.

  1999cites this paper
• Further characteristics of the Ca(2+)-inactivated Cl(-) channel in Xenopus laevis oocytes.

  1999cites this paper
• Endogenous Ion Channels in Oocytes of Xenopus laevis: Recent Developments

  1999cites this paper
• Oligomerization state of MIP proteins expressed in Xenopus oocytes as revealed by freeze-fracture electron-microscopy analysis.

  1999cites this paper
• Expression of membrane transporters in cane toad Bufo marinus oocytes.

  1999cites this paper
• Identification of a K+ channel from potato leaves by functional expression in Xenopus oocytes.

  1998cites this paper
• pICln can regulate swelling-induced Cl- currents in either layer of rabbit ciliary epithelium.

  1998cites this paper
• Functional characterization of an organic cation transporter (hOCT1) in a transiently transfected human cell line (HeLa).

  1998cites this paper
• A Yeast Recombinant Aquaporin Mutant That Is Not Expressed or Mistargeted in Xenopus Oocyte Can Be Functionally Analyzed in Reconstituted Proteoliposomes*

  1998cites this paper
• Characterization of SKT1, an inwardly rectifying potassium channel from potato, by heterologous expression in insect cells.

  1998cites this paper
• Chlorpromazine activates chloride currents in Xenopus oocytes.

  1997cites this paper
• Expression of a non-MDR2-coded liver phosphatidylcholine membrane transport protein in Xenopus laevis oocytes.

  1997cites this paper
• A site‐directed mutagenesis study on the conserved alanine residue in the distal third intracellular loops of cholecystokininB and neurotensin receptors

  1997cites this paper
• The Ca2+-inactivated Cl− Channel at Work: Selectivity, Blocker Kinetics and Transport Visualization

  1997cites this paper
• Basic Amino Acids at the C‐Terminus of the Third Intracellular Loop Are Required for the Activation of Phospholipase C by Cholecystokinin‐B Receptors

  1997cites this paper
• A pH-sensitive Yeast Outward Rectifier K Channel with Two Pore Domains and Novel Gating Properties (*)

  1996cites this paper
• Incorporation of proteins into (Xenopus) oocytes by proteoliposome microinjection: functional characterization of a novel aquaporin.

  1996cites this paper
• Detection of intracellular calcium elevations in Xenopus laevis oocytes: aequorin luminescence versus electrophysiology.

  1996cites this paper
• Expression of the renal Na+/dicarboxylate cotransporter, NaDC-1, in COS-7 cells

  1996cites this paper
• Characterization of a new high‐temperature‐induced 66‐kDa heat‐shock protein, antigenically related to heat‐shock protein 72

  1996cites this paper
• Homomeric beta 1 gamma-aminobutyric acid A receptor-ion channels: evaluation of pharmacological and physiological properties.

  1996cites this paper
• Molecular cloning and functional expression of the alpha1A-adrenoceptor of Medaka fish, Oryzias latipes.

  1996cites this paper
• Effects of P2-purinoceptor antagonists on degradation of adenine nucleotides by ecto-nucleotidases in folliculated oocytes of Xenopus laevis.

  1996cites this paper
• Characteristics of ecto-ATPase ofXenopus oocytes and the inhibitory actions of suramin on ATP breakdown

  1996cites this paper
• Functional reconstitution of KCl‐evoked, Ca2+‐dependent acetylcholine release system in Xenopus oocytes microinjected with presynaptic plasma membranes and synaptic vesicles

  1996cites this paper
• Further investigation of ionic diffusive properties and of NH4+ pathways inXenopus laevis oocyte cell membrane

  1996cites this paper
• Water channels encoded by mutant aquaporin-2 genes in nephrogenic diabetes insipidus are impaired in their cellular routing.

  1995cites this paper
• Amiloride-sensitive Na+ conductance in native Xenopus oocytes.

  1995cites this paper