STUDIES OF PHAGOCYTOSIS OF GROUP A STREPTOCOCCI BY POLYMORPHONUCLEAR LEUCOCYTES IN VITRO

Studies have been made on phagocytosis and killing of Group A streptococci during mixing with suspensions of leucocytes in vitro. Under appropriate test conditions an anti-phagocytic effect can be demonstrated for the streptococcal hyaluronic acid capsule as well as for its M protein. The results obtained suggest an explanation for the suitability of human, but not rabbit, blood for opsonophagocytic tests designed to measure type-specific streptococcal antibodies. Human sera contain a factor which counteracts the anti-phagocytic effects of streptococcal hyaluronic acid capsules, and hence human blood serves well for detection of antibodies which combine with the only other phagocytosis-resisting component of this microorganism, namely M protein. In contrast, rabbit sera contain none of this factor, and addition of antibody to M protein to phagocytic test systems employing rabbit serum does not necessarily render the streptococci susceptible to engulfment by white cells, since the hyaluronic acid capsule may continue to interfere with phagocytosis. The nature of the human serum factor which opsonizes encapsulated streptococci is unknown. It does not appear to be an antibody or an enzyme capable of depolymerizing hyaluronic acid.

• Publication year

  1960

• Venue

  Journal of Experimental Medicine

• Publication date

  1960-02-29

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.111.3.309PMID 13714578PMCID 2137261
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• STUDIES ON THE PATHOGENICITY OF GROUP A STREPTOCOCCI

  1959cited by this paper
• The lack of bactericidal effect of mouse blood for nonvirulent group A streptococci.

  1959influential reference
• STUDIES ON THE PATHOGENICITY OF GROUP A STREPTOCOCCI

  1959cited by this paper
• ACCESSORY PLASMA FACTORS INVOLVED IN THE BACTERICIDAL TEST FOR TYPE-SPECIFIC ANTIBODY TO GROUP A STREPTOCOCCI

  1958cited by this paper
• FATE OF NON-VIRULENT GROUP A STREPTOCOCCI PHAGOCYTIZED BY HUMAN AND MOUSE NEUTROPHILS

  1957cited by this paper
• Mouse protection and enhancement of phagocytosis by antisera to Streptococcus pyogenes.

  1956cited by this paper
• Mouse protection and enhancement of phagocytosis by antisera to Streptococcus pyogenes.

  1956influential reference
• PHAGOCYTIN: A BACTERICIDAL SUBSTANCE FROM POLYMORPHONUCLEAR LEUCOCYTES

  1956cited by this paper
• THE RELATIONSHIP OF M PROTEIN AND RESISTANCE TO PHAGOCYTOSIS IN THE BETA HEMOLYTIC STREPTOCOCCI

  1955cited by this paper
• PROTECTIVE EFFECT OF HYALURONIDASE AND TYPE-SPECIFIC ANTI-M SERUM ON EXPERIMENTAL GROUP A STREPTOCOCCUS INFECTIONS IN MICE

  1948cited by this paper
• BACTERIOSTATIC EFFECT OF HUMAN SERA ON GROUP A STREPTOCOCCI

  1945cited by this paper
• BACTERIOSTATIC EFFECT OF HUMAN SERA ON GROUP A STREPTOCOCCI

  1945influential reference
• THE RÔLE OF THE MUCOID POLYSACCHARIDE (HYALURONIC ACID) IN THE VIRULENCE OF GROUP A HEMOLYTIC STREPTOCOCCI

  1944cited by this paper
• THE OCCURRENCE OF BACTERIOSTATIC PROPERTIES IN THE BLOOD OF PATIENTS AFTER RECOVERY FROM STREPTOCOCCAL PHARYNGITIS.

  1944cited by this paper
• Factors which determine the fate of hæmolytic streptococci (group A) in shed blood and in serum

  1939cited by this paper

• The historical milestones in the understanding of leukocyte biology initiated by Elie Metchnikoff

  2011cites this paper
• ROLE OF OXYGEN FREE RADICALS IN RETINAL DAMAGE ASSOCIATED WITH EXPERIMENTAL UVEITIS*

  2005cites this paper
• STERNAL MARROW SPLEEN LYMPH NODE of LDH isoenzyme patterns from normal 862 STARKWEATHEII , SPENCER AND SCHOGI

  2005cites this paper
• The Lactate Dehydrogenases of Hemopoietic Cells

  2005cites this paper
• Proceedings of the British Pharmacological Society

  2004cites this paper
• ACCESSORY PLASMA FACTORS INVOLVED IN THE BACTERICIDAL TEST FOR TYPE-SPECIFIC ANTIBODY TO GROUP A STREPTOCOCCI* I. ATYPICAL BEHAVIOR OF SOME HUMAN AND RABBIT BLOODS

  2003influential citation
• ACCESSORY PLASMA FACTORS INVOLVED IN THE BACTERICIDAL TEST FOR TYPE-SPECIFIC ANTIBODY TO GROUP A STREPTOCOCCI

  2003influential citation
• RESULTS Generation of Chemotactic Activity for Rabbit PMN in Guinea Pig Peritoneal Exudates

  2003cites this paper
• Degranulation of Rabbit Polymorphonuclear Leucocytes Following Phagocytosis of Various Microorganisms

  2003cites this paper
• Commentary on and reprint of Boyden S, The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes, in Journal of Experimental Medicine (1962) 115:453–457

  2000cites this paper
• BASIS FOR I.MMUNITY TO TYPHOID IN MICE AND THE QUESTION OF "CELLULAR IMMIUNITY"

  1999cites this paper
• Genetic Inactivation of an Extracellular Cysteine Protease (SpeB) Expressed by Streptococcus pyogenes Decreases Resistance to Phagocytosis and Dissemination to Organs

  1998cites this paper
• Expression of both M protein and hyaluronic acid capsule by group A streptococcal strains results in a high virulence for chicken embryos

  1996cites this paper
• Hyaluronate capsule and surface M protein in resistance to opsonization of group A streptococci

  1996influential citation
• Enhanced chemiluminescence with decreased antibody-dependent cellular cytotoxicity of human alveolar neutrophil in patients with adult respiratory distress syndrome.

  1992cites this paper
• Lipid peroxidation in experimental uveitis: sequential studies.

  1992cites this paper
• Molecular characterization of a locus required for hyaluronic acid capsule production in group A streptococci

  1992cites this paper
• Role of oxygen free radicals in retinal damage associated with experimental uveitis.

  1990cites this paper
• Cross-reactivity between streptococcus and human tissue: a model of molecular mimicry and autoimmunity.

  1989cites this paper
• Streptococcal M protein: molecular design and biological behavior

  1989cites this paper
• Molecular aspects of the phagocytosis resistance of group a streptococci

  1988cites this paper
• Induction of antibodies to hyaluronic acid by immunization of rabbits with encapsulated streptococci

  1986cites this paper
• The Role of the Neutrophil in the Inflammatory Response

  1985cites this paper
• Arachidonic acid metabolism in a cell suspension isolated from rabbit renal outer medulla.

  1984cites this paper
• The cytotoxic action in vitro of catalytically sulphurated unsaturated fatty acids on Ehrlich's ascites cancer and normal peritoneal exudate (leucocytes).

  1984cites this paper
• The Adjuvant Effect of Peritoneal Fluid in Experimental Peritonitis: Mechanism and Clinical Implications

  1984cites this paper
• Phagocytosis of microorganisms.

  1982cites this paper
• Virulence factors in streptococci.

  1982cites this paper
• Studies of glycogen-induced inflammation of mice. Dynamics of inflammatory responses and influence of antiinflammatory drugs and protease inhibitors.

  1982cites this paper
• Studies on glycogen-induced inflammation of mice

  1982cites this paper
• A radioimmunoassay for leukotriene B4.

  1982cites this paper
• Septicaemia: A Symposium Held in Stockholm, Sweden September 5–6, 1980

  1982cites this paper
• Hyaluronate capsule prevents attachment of group A streptococci to mouse peritoneal macrophages

  1981cites this paper
• Immunology of Streptococci

  1981cites this paper
• Enhanced intracellular killing ofStaphylococcus aureus by canine monocytes treated with liposomes containing amikacin, gentamicin, kanamycin, and tobramycin

  1981cites this paper
• Enhanced phagocytosis of group A streptococci M type 6 by oleic acid.

  1981cites this paper
• Comparable phagocytic activity of rhesus monkey and human blood polymorphonuclear leucocytes

  1981cites this paper
• Chemokinetic activity of arachidonic and lipoxygenase products on leuocyctes of different species.

  1980cites this paper
• Studies on alternative pathway in systemic lupus erythematosus-Using the chemotaxis assay-

  1980cites this paper
• alpha-Haemolysin: an additive virulence factor in Escherichia coli.

  1980cites this paper
• Chemotactic factors as cause of polyleukocyte urine in urinary tract infection: chemotactic activity of urinary protein fraction.

  1980cites this paper
• A New Quantitative Fluorimetric Assay for Phagocytosis of Bacteria

  1980cites this paper
• Attachment of polymorphonuclear leukocytes to glomeruli with immune deposits.

  1979cites this paper
• Responses of the mouse vas deferens to dopamine and some related drugs [proceedings].

  1978cites this paper
• PROCEEDINGS OF THE British Pharmacological Society

  1978influential citation
• Phagocytosis: a review.

  1978cites this paper
• The release of thromboxane B2 by rabbit peritoneal polymorphonuclear leucocytes [proceedings].

  1978influential citation
• Influence of a deficiency of the second component of complement on the bactericidal activity of neutrophils in vitro.

  1977cites this paper
• Inhibition of leukocyte chemotaxis by homogenates of tumor tissues.

  1977cites this paper
• Cytotoxic effect of fatty acids in vitro on Ehrlich exudative carcinoma cells and normal exudative cells (leukocytes).

  1977cites this paper
• The polymorph transfer reaction: a model system for the study of acute inflammation [proceedings].

  1976cites this paper
• Generation of thromboxane A2-like activity from prostaglandin endoperoxides by polymorphonuclear leukocyte homogenates [proceedings].

  1976cites this paper
• An evaluation of the role of leukocytes in the pathogenesis of experimentally induced corneal vascularization. III. Studies related to the vasoproliferative capability of polymorphonuclear leukocytes and lymphocytes.

  1976cites this paper
• PROCEEDINGS OF THE British Pharmacological Society 15th‐16th July, 1976 UNIVERSITY OF DUNDEE COMMUNICATIONS

  1976cites this paper
• Polymorphonuclear leukocytes produce thromboxane A2-like activity during phagocytosis.

  1976cites this paper
• Immunity to the group B streptococci: interaction of serum and macrophages with types Ia, Ib, and Ic

  1976cites this paper
• A CHEMOTACTIC ROLE FOR PROSTAGLANDINS RELEASED FROM POLYMORPHONUCLEAR LEUCOCYTES DURING PHAGOCYTOSIS

  1975cites this paper
• Observations on phagocytosis of urate crystals by polymorphonuclear leucocytes.

  1975cites this paper
• Phagocytosis: recognition and ingestion.

  1975cites this paper
• Effect of oral hygiene on clearance of soluble chemotactic bacterial products from human saliva.

  1974cites this paper
• Chapter 5 – CHEMOTAXIS

  1974cites this paper
• Chapter 6 – PHAGOCYTOSIS

  1974cites this paper
• Proceedings: Effects of neuraminidase and N-acetyl neuraminic acid on the adhesion of circulating granulocytes and platelets in venules.

  1973cites this paper
• The role of complement in immune phagocytosis of group A streptococci by mouse peritoneal phagocytic cells.

  1973cites this paper
• [Detection of type-specific streptococcal M-antibodies in the indirect bactericidal test by use of monkey blood].

  1972cites this paper
• Proceedings: Prostaglandin production by rabbit peritoneal polymorphonuclear leukocytes in vitro.

  1972cites this paper
• Mechanisms of cell and tissue injury induced by group A streptococci: relation to poststreptococcal sequelae.

  1972cites this paper
• The relationship of M protein of group a streptococci to mouse virulence.

  1972cites this paper
• Proceedings: Comparison of a coronary vasodilator drug (carbochromen) and a cardiac stimulant (oxyfedrin) on blood flow and oxygen extraction in experimental myocardial infarcts.

  1972cites this paper
• Immunoglobulins as aspecific opsonins. II. The influence of specific and aspecific immunoglobulins on the in vitro phagocytosis of noncapsulated, capsulated, and decapsulated bacteria by human neutrophils.

  1971cites this paper
• MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF GLYCOGEN PARTICLES ISOLATED FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES

  1971cites this paper
• Chemotactic Activity Generated by Staphylococcal Enterotoxin B

  1971cites this paper
• PROSTAGLANDIN E1: A POTENTIAL MEDIATOR OF THE INFLAMMATORY RESPONSE

  1971cites this paper
• Antigenic properties of Bacillus licheniformis cell wall components.

  1971cites this paper
• BIOLOGICAL ACTIVITY OF COMPLEMENT IN VIVO

  1971cites this paper
• THE RELATIONSHIP BETWEEN GROUP A AND GROUP C MENINGOCOCCAL POLYSACCHARIDES AND SERUM OPSONINS IN MAN

  1970cites this paper
• Participation of nonspecific factors in the destruction of type M 12 streptococcus in vitro. I. Nonspecific absorption of anti-M 12 bactericidal activity.

  1970cites this paper
• Studies on eosinophil leucocyte migration. II. Factors specifically chemotactic for eosinophils and neutrophils generated from guinea-pig serum by antigen-antibody complexes.

  1970cites this paper
• Biological activity of papain digeted streptococcal anti-M antibody.

  1969cites this paper
• Studies on chemotaxis. XII. Generation of chemotactic activity for polymorphonuclear leucocytes in sera with complement dificiencies.

  1969cites this paper
• Phagocytic activity of leukemic myeloblasts.

  1969cites this paper
• Identification of type specific streptococcal antibodies by in vitro phagocytosis.

  1969cites this paper
• Intraphagocytic Degradation of Group A Streptococci: Electron Microscopic Studies

  1969cites this paper
• Nephritogenic and rheumatogenic group A streptococci.

  1969cites this paper
• Chronic granulomatous disease in three female siblings.

  1968cites this paper
• Improved tests for the evaluation of neutrophil function in human disease.

  1968cites this paper
• Improved method for obtaining polymorphonuclear leucocytes from guinea pigs.

  1968cites this paper
• ULTRASTRUCTURE OF HUMAN LEUKOCYTES AFTER SIMULTANEOUS FIXATION WITH GLUTARALDEHYDE AND OSMIUM TETROXIDE AND "POSTFIXATION" IN URANYL ACETATE

  1968cites this paper
• The Effects of Norethynodrel and Mestranol on the Growth and Virulence of Staphylococcus aureus

  1968cites this paper
• [Cellular modifications and phagocytosis in streptococcal peritoneal infection in mice. I. Cellular reactions after intraperitoneal inoculation of a strain of group A, type 24, of different degrees of virulence].

  1968cites this paper
• INTERACTIONS OF THE COMPLEMENT SYSTEM WITH ENDOTOXIC LIPOPOLYSACCHARIDE

  1968cites this paper
• The fate of group A streptococci following phagocytosis. In vitro phagocytic studies of isotope-labeled streptococci.

  1967cites this paper
• Opsonization of group A streptococci by complement deficient blood from a patient with hereditary angioneurotic edema.

  1967cites this paper
• Mimetic relationships between group A streptococci and mammalian tissues.

  1967cites this paper
• The Inhibition of Hepatic Protein Synthesis by Some Oral Hypoglycemic Agents

  1967cites this paper
• The lactate dehydrogenases of hemopoietic cells.

  1966cites this paper
• Fatal granulomatous disease of childhood. An inborn abnormality of phagocytic function.

  1966cites this paper
• Reversal of Chemotaxis in vitro and Chemotactic Activity of Leukocyte Fractions.∗

  1966cites this paper
• STUDIES ON HUMAN LEUKOCYTE MOTILITY

  1966cites this paper
• Response to inbred mice to virulent and avirulent Salmonella typhimurium and their endotoxins.

  1966cites this paper