NF-κB/Rel Proteins Are Required for Neuronal Differentiation of SH-SY5Y Neuroblastoma Cells*

The expression, cellular localization, and activation of the NF-κB/Rel transcription factors are altered during neuronal differentiation, but the significance is unclear. Here we investigate the requirement for NF-κB/Rel proteins in neuronal differentiation. SH-SY5Y neuroblastoma cells were induced to differentiate with retinoic acid (RA) or 12-O-tetradecanoylphorbol 13-acetate (TPA), and differentiation was demonstrated by morphological criteria and the enhanced expression of Bcl-2. NF-κB was transiently activated after the addition of the differentiation inducers before the morphological signs of differentiation and the enhanced Bcl-2 synthesis. The onset of NF-κB activation coincided with a significant reduction in the amount of only one of four NF-κB-inhibitory proteins examined (I-κBβ). In contrast, NF-κB activation and the reduction in I-κBβ failed to occur in SH-SY5Y cells transformed with I-κBαM, a dominant-negative inhibitor of NF-κB/Rel proteins. These I-κBαM-expressing cells failed to differentiate into neuronal cell types when treated with RA or TPA, and the increased Bcl-2 synthesis was blocked. Therefore, NF-κB/Rel proteins are required for neuronal differentiation of SH-SY5Y neuroblastoma cells.

• Publication year

  1999

• Venue

  Journal of Biological Chemistry

• Publication date

  1999-10-22

• Fields of study

  Biology

• Identifiers
  DOI 10.1074/jbc.274.43.30341
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• NF-κB/Rel proteins are required for neuronal differentiation of SH-SY5Y neuroblastoma cells.

  Confidence 0.99

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• Cells expressing the dominant-negative inhibitor I-κBαM fail to activate NF-κB, fail to reduce I-κBβ, and do not differentiate in response to retinoic acid or 12-O-tetradecanoylphorbol 13-acetate.

  Confidence 0.97

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• NF-κB activation coincides with a reduction in I-κBβ, while the other NF-κB-inhibitory proteins examined do not show the same change.

  Confidence 0.92

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• NF-κB is transiently activated after retinoic acid or 12-O-tetradecanoylphorbol 13-acetate treatment before the morphological signs of differentiation and the increase in Bcl-2 expression.

  Confidence 0.98

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• 12-o-tetradecanoylphorbol 13-acetate

  compound, differentiation inducer

  A phorbol ester used as an alternative differentiation-inducing treatment for SH-SY5Y cells.

  Aliases: TPA

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• bcl-2

  protein, marker

  An anti-apoptotic protein whose increased expression served as a marker of differentiation in the treated cells.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• i-κbαm

  protein, mutant inhibitor

  A dominant-negative mutant inhibitor used to block NF-κB/Rel signaling in transformed SH-SY5Y cells.

  Aliases: IκBαM

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• i-κbβ

  protein, inhibitor

  One of the NF-κB inhibitory proteins examined for changes during differentiation-inducing treatment.

  Aliases: IκBβ

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• neuronal differentiation

  cellular process

  The process by which SH-SY5Y cells acquire neuronal morphology and markers under the induced conditions used here.

  Aliases: differentiation into neuronal cell types

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• nf-κb/rel proteins

  protein family, transcription factor

  A family of transcription factors studied here for their role in regulating neuronal differentiation in SH-SY5Y cells.

  Aliases: NF-κB, Rel proteins

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• retinoic acid

  compound, differentiation inducer

  A differentiation-inducing compound applied to SH-SY5Y cells in the experiment.

  Aliases: RA

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• sh-sy5y neuroblastoma cells

  cell line

  A human neuroblastoma cell line used as the differentiation model in this experiment.

  Aliases: SH-SY5Y cells

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review

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