A Rapid HPLC Method for Separation and Determination of Major Organic Acids in Grape Musts and Wines

An isocratic high performance liquid chromatographic (HPLC) method was developed for the separation and quantitative analysis of the major carboxylic acids of grape musts and wines such as citric, tartaric, malic, lactic, and acetic acids. By using an Aminex HPX87-H, a strong cation exchange resin column eluted with dilute sulfuric acid, and monitoring with uv at 210 nm, sample preparation is reduced to simple membrane filtration for white wines or musts and to the removal of phenolic compounds employing disposable reverse phase cartridges for red wines. The use of mobile phase at a different pH for grape must and wine sample analysis allows the separation of compounds such as malic acid and fructose that normally coelute. The results of the HPLC analyses were compared with data obtained by other methods in a range of white wine, red wine, and must samples.

• Publication year

  1987

• Venue

  American Journal of Enology and Viticulture

• Publication date

  Unknown publication date

• Fields of study

  Chemistry, Environmental Science

• Identifiers
  DOI 10.5344/ajev.1987.38.2.151
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• No references are available for this paper.

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