HPLC analysis of vitamin B6 in foods

B 6 -vitamiini (pyridoksiini) on vesiliukoinen vitamiini,joka esiintyy erilaisissa kemiallisissa muodoissa biologisissa materiaaleissa kuten elainkudoksissa, kasvisolukoissa ja niita sisaltavissa elintarvikkeissa. Elimistossa B 6 -vitamiini edesauttaa monia biokemiallisia reaktioita mm. valkuaisaineiden ja glykogeenin aineenvaihdunnassa. Perinteisen vitamiinivaikutuksen lisaksi pyridoksiini saattaa liittya mm. altistumiseen sydan- ja verisuonitaudeille. Tieteellista nayttoa naista uusista yhteyksista ei kuitenkaan viela ole saatu. Perinteisesti B 6 -vitamiini on maaritetty elintarvikkeista mikrobiologisesti, jolloin pyridoksiini on vapautettu ns. sidotuista muodoista. Tassa tyossa selvitettiin nestekromatografian (HPLC:n) soveltuvuutta B 6 -vitamiinin vapaiden ja sidottujen muotojen maarittamiseen elintarvikkeista. Kehitetyn menetelman luotettavuus varmistettiin mm. Euroopan unionin jarjestamissa laboratorioiden valisissa vertailututkimuksissa. Menetelmalla maaritettiin 50 elintarvikenimikkeen eri B 6 -vitamiinimuotojen pitoisuudet. Runsaasti pyridoksiinia tai sen johdannaisia sisalsivat mm. liha ja sisaelimet (maksa ja munuaiset), kananmunan keltuainen ja hasselpahkina. Kasviperaisten elintarvikkeiden B 6 -vitamiini koostui suurelta osin sidotuista glykosyloiduista vitamiinimuodoista. Niiden hyvaksikaytettavyydesta elimistossa on varsin ristiriitaisia tietoja. Tyossa saadut elintarvikkeiden B 6 -vitamiinitulokset liitetaan Kansanterveyslaitoksen elintarvikkeiden koostumustietopankkiin ja niita hyodynnetaan mm. ravitsemussuunnittelussa. Tyo osoitti olevan tarpeellista yhdenmukaistaa kaytettavia maaritysmenetelmia, jotta eri laboratorioissa saadut tulokset olisivat paremmin vertailukelpoisia. Tata menetelmien yhtenaistamista jatketaan mm. Euroopan Standardoimisliiton (CEN) tyoryhmissa.

• Publication year

  1999

• Venue

  Agricultural and Food Science

• Publication date

  1999-01-06

• Fields of study

  Not labeled

• Identifiers
  DOI 10.23986/AFSCI.5632
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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