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Sperm DNA Damage Before and After Kidney Transplantation

A. Javadneia,M. T. Moghadam,A. Alivand,Alireza Kheradmand,M. Zargar,S. Sabbagh

Published 2019 in Nephro-Urology Monthly

ABSTRACT

Background: Thirty-six people per one million have end-stage renal disease (ESRD). ESRD has the indication of kidney transplantation; or hemodialysis and it is one of the causes of infertility in men. Objectives: A high prevalence of sperm DNA damage is reported in men’s semen with ESRD; therefore, the aim of this study was to evaluate sperm DNA damage in men with ESRD before and after kidney transplantation. Methods: Thisstudyevaluated15menwithchronicrenalfailureandwerecandidatesforkidneytransplantation. DNAdamageand semenparameterswereinvestigatedinthesemenof thesepatientsbeforeandafterthetransplantation. Anilineblueandtoluidine bluestainingwereusedtoevaluatethespermchromatinstructureandcondensation. Theslideswereanalyzedbylightmicroscope to determine the percentage of DNA damage in sperms. Results: Thepercentageof positiveanilineblueandtoluidinebluesignificantlydecreasedafterkidneytransplantation(40% versus 29%,P=0.01foranilineblueand45% versus35%,P=0.01fortoluidineblue). Spermmorphology,count,motility,andspermvolume were improved after the transplantation; except for the morphology, all of them were statically significant. Conclusions: Thesefindingssupportthatthekidneytransplantationinpatientswithrenalfailurecouldimprovechromatinstruc-ture and condensation and sperm parameters. Additionally both toluidine blue and aniline blue staining are simple and suitable techniques to evaluate DNA damage.

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