Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Andrologia

• Publication date

  2012-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1111/j.1439-0272.2011.01206.xPMID 21806662
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Y chromosome microdeletions, sperm DNA fragmentation and sperm oxidative stress as causes of recurrent spontaneous abortion of unknown etiology.

  2010cited by this paper
• Role of male factor in early recurrent embryo loss: do antioxidants have any effect?

  2009cited by this paper
• Evaluation of male fertility potential by Toluidine Blue test for sperm chromatin structure assessment.

  2009cited by this paper
• Chromomycin A3 staining, sperm chromatin structure assay and hyaluronic acid binding assay as predictors for assisted reproductive outcome.

  2009cited by this paper
• Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion

  2009cited by this paper
• Is abnormal sperm function an indicator among couples with recurrent pregnancy loss?

  2008cited by this paper
• Data analysis of two in vivo fertility studies using Sperm Chromatin Structure Assay-derived DNA fragmentation index vs. pregnancy outcome.

  2008cited by this paper
• Journal of Experimental & Clinical Assisted Reproduction Paternal Effects on Early Embryogenesis

  2008cited by this paper
• Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests

  2008cited by this paper
• Association of various sperm parameters with unexplained repeated early pregnancy loss—which is most important?

  2007cited by this paper
• Clinical relevance of sperm DNA damage in assisted reproduction.

  2007cited by this paper
• Association between fertilin beta, protamines 1 and 2 and spermatid-specific linker histone H1-like protein mRNA levels, fertilization ability of human spermatozoa, and quality of preimplantation embryos.

  2007cited by this paper
• Altered protamine expression and diminished spermatogenesis: what is the link?

  2007cited by this paper
• The impact of male factor on recurrent pregnancy loss

  2007cited by this paper
• Assessment of nuclear DNA integrity of epididymal spermatozoa following experimental chronic spinal cord injury in the rat.

  2007cited by this paper
• Comparison of chromatin assays for DNA fragmentation evaluation in human sperm.

  2006cited by this paper
• Protamines and male infertility.

  2006cited by this paper
• Recurrent miscarriage.

  2006cited by this paper
• Recurrent miscarriage Patience and possibly lower concentrations of luteinising hormone before conception are needed

  2006cited by this paper
• Sperm protamine 1/protamine 2 ratios are related to in vitro fertilization pregnancy rates and predictive of fertilization ability.

  2006cited by this paper
• Decline in fertility of mouse sperm with abnormal chromatin during epididymal passage as revealed by ICSI.

  2005cited by this paper
• Potential adverse effect of sperm DNA damage on embryo quality after ICSI.

  2005cited by this paper
• WHO Laboratory Manual for the Examination of Human Semen and Sperm-cervical Mucus Interaction 4th Edition [Book Review]

  2005cited by this paper
• Recurrent miscarriage

  2005cited by this paper
• Identification and evaluation of a novel sperm protamine abnormality in a population of infertile males.

  2005cited by this paper
• Sperm aneuploidy and recurrent pregnancy loss.

  2004cited by this paper
• Toluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays.

  2004cited by this paper
• Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization.

  2004cited by this paper
• Human protamines and the developing spermatid: their structure, function, expression and relationship with male infertility.

  2003cited by this paper
• Elevated Sperm Chromosome Aneuploidy and Apoptosis in Patients With Unexplained Recurrent Pregnancy Loss

  2003cited by this paper
• Toluidine blue test for sperm DNA integrity and elaboration of image cytometry algorithm

  2003cited by this paper
• SPERM DNA FRAGMENTATION IS INCREASED IN COUPLES WITH UNEXPLAINED RECURRENT PREGNANCY LOSS

  2003cited by this paper
• Oxidative DNA damage in human sperm influences time to pregnancy.

  2003cited by this paper
• Expression of mammalian spermatozoal nucleoproteins

  2003cited by this paper
• The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development.

  2002cited by this paper
• Sperm Single-Stranded DNA, Detected by Acridine Orange Staining, Reduces Fertilization and Quality of ICSI-Derived Embryos

  2002cited by this paper
• Generalized abnormal embryonic development in missed abortion: embryoscopic and cytogenetic findings.

  2002cited by this paper
• Paternal effects acting during the first cell cycle of human preimplantation development after ICSI.

  2002cited by this paper
• Free thiols in human spermatozoa: correlation with sperm DNA integrity.

  2001cited by this paper
• Altered protamine 2 expression is uncommon in donors of known fertility, but common among men with poor fertilizing capacity, and may reflect other abnormalities of spermiogenesis.

  2001cited by this paper
• Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men.

  2001cited by this paper
• Paternal contributions to the mammalian zygote: fertilization after sperm-egg fusion.

  2000cited by this paper
• Poor quality of sperm as it affects repeated early pregnancy loss.

  2000cited by this paper
• World Health Organization Laboratory Manual for the Examination of Human Semen and Sperm‐Cervical Mucus Interaction, 4th ed.

  2000cited by this paper
• Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic.

  1999cited by this paper
• Use of acridine orange to evaluate chromatin integrity of human spermatozoa in different groups of infertile men

  1999cited by this paper
• Origin of DNA damage in ejaculated human spermatozoa.

  1999cited by this paper
• Use of clomiphene citrate in the treatment of men with high sperm chromatin stability.

  1998cited by this paper
• A logistic regression model including DNA status and morphology of spermatozoa for prediction of fertilization in vitro.

  1998cited by this paper
• The relationship between sperm morphology and rates of fertilization, pregnancy and spontaneous abortion in an in-vitro fertilization/intracytoplasmic sperm injection programme.

  1997cited by this paper
• Chromomycin A3-staining as an indicator of protamine deficiency and fertilization.

  1996cited by this paper
• The relationship between acridine orange fluorescence of sperm nuclei and the fertilizing ability of human sperm.

  1996cited by this paper
• Semen parameters and sperm morphology in men in unexplained recurrent spontaneous abortion, before and during a 3 year follow-up period.

  1996cited by this paper
• Higher order nuclear structure in mammalian sperm revealed by in situ hybridization and extended chromatin fibers.

  1995cited by this paper
• Relationship between the presence of endogenous nicks and sperm chromatin packaging in maturing and fertilizing mouse spermatozoa.

  1995cited by this paper
• Presence of endogenous nicks in DNA of ejaculated human spermatozoa and its relationship to chromomycin A3 accessibility.

  1995cited by this paper
• Evidence for a strong paternal effect on human preimplantation embryo development and blastocyst formation

  1994cited by this paper
• Thiol-disulfide status and acridine orange fluorescence of mammalian sperm nuclei.

  1992cited by this paper
• Sperm nuclear instability and staining with aniline blue: abnormal persistence of histones in spermatozoa in infertile men.

  1992cited by this paper
• Aniline blue staining as a marker of sperm chromatin defects associated with different semen characteristics discriminates between proven fertile and suspected infertile men.

  1990cited by this paper
• Changes in human sperm chromatin stability during preparation for in-vitro fertilization.

  1990cited by this paper
• Influence of past reproductive performance on risk of spontaneous abortion.

  1989influential reference
• Factors leading to tripronucleate eggs during human in-vitro fertilization.

  1986cited by this paper
• A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence.

  1984cited by this paper
• The formation of disulfide bonds in human protamines during sperm maturation

  1979cited by this paper

• Dose- and size-dependent effects of Fe3O4 nanoparticles on bull sperm cryopreservation.

  2026cites this paper
• Evaluation of AFP III-Conjugated Fe3O4 Nanoparticles on Bull Sperm Cryopreservation.

  2025cites this paper
• Fisetin attenuates the adverse effects of freezing and thawing procedures on the biological characteristics of human asthenoteratozoospermia samples.

  2025cites this paper
• Protective Effects of Rutin Against Docetaxel-Induced Testicular Damage in Rats: Effects on Antioxidant Defence, Apoptosis and Autophagy.

  2025cites this paper
• THE PHARMACOLOGICAL AND ADVERSE EFFECTS OF THE BETEL NUT ON EXPERIMENTAL ANIMALS

  2025cites this paper
• Farelere Uygulanan Paklitaksel’in Testis Dokusu Üzerine Etkileri

  2025cites this paper
• Lipid mixtures (from a liposome kit) and melatonin improve post-thawed Angora goat sperm parameters.

  2024cites this paper
• Comparison between open and closed systems for vitrification of individual sperm: assessing morphometric measurements and chromatin integrity

  2024cites this paper
• Male Contributory Factors in Recurrent Pregnancy Loss

  2023cites this paper
• Human Sperm Morphology as a Marker of Its Nuclear Quality and Epigenetic Pattern

  2022cites this paper
• Effects of rosiglitazone and vitamin E on testicular tissue and sperm parameters in propylthiouracil-induced hypothyroidism in rats

  2022cites this paper
• The effects of morphine abuse on sperm parameters, chromatin integrity and apoptosis in men

  2022cites this paper
• Advanced sperm tests and impact on clinical male factor management

  2022cites this paper
• Improvement of human sperm properties with platelet-rich plasma as a cryoprotectant supplement

  2022cites this paper
• Detection of DNA damage by SCD and Rate of Apoptosis DNA by Gel Electrophoresis among infertile males

  2022cites this paper
• Dose dependent intra-testicular accumulation of silver nanoparticles triggers morphometric changes in seminiferous tubules and Leydig cells and changes the structural integrity of spermatozoa chromatin.

  2022cites this paper
• EVALUATION OF IN VITRO FERTILIZATION POTENTIAL AND SPERM PARAMETERS IN ACRYLAMIDE TREATED MICE

  2022cites this paper
• Evaluation of sperm chromatin/DNA integrity, morphology, and Catsper expression on diabetic C57BL/6 mice.

  2022cites this paper
• ANXA2, SP17, SERPINA5, PRDX2 genes, and sperm DNA fragmentation differentially represented in male partners of infertile couples with normal and abnormal sperm parameters

  2022cites this paper
• Male factor testing in recurrent pregnancy loss cases: A narrative review

  2022cites this paper
• Comparison of sperm preparation methods to improve the recovery of mature spermatozoa in sub-fertile males.

  2022cites this paper
• The association of vitamin D with semen quality and fertility hormones in idiopathic recurrent pregnancy loss without the female factor

  2021cites this paper
• THE REPRODUCTIVE EFFECT OF LIPOSOMAL CIMETIDINE ON MALE MICE

  2021cites this paper
• Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia

  2021cites this paper
• Effect of Sperm Dna Fragmentation Index on Clinical Outcomes of Intra-Uterine Insemination Patients

  2021cites this paper
• Metabolic Syndrome and Reproduction

  2021cites this paper
• Sperm Chromatin Structure: Toluidine Blue Staining

  2021cites this paper
• Aerobic training mitigates the negative impact of diabetes on fertility

  2021cites this paper
• Impacts administration of Rifampicin on sperm DNA integrity and Male Reproductive System parameters in rats

  2021cites this paper
• Genotoxic activity of betel nut on germinal cell in Sarcoma 180 ascites tumour bearing male mice

  2021cites this paper
• L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study

  2020cites this paper
• Comparison of male reproductive parameters in mice with type 1 and type 2 diabetes

  2020cites this paper
• Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy

  2020cites this paper
• Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds.

  2020cites this paper
• Spermatozoal Chromatin Structure: Role in Sperm Functions and Fertilization

  2020cites this paper
• Effect of Selenium and Electrical Stimulation on the Expression of Cation Channel Spermia 1 and 2 Genes in Spinal Cord Injury Rat Model

  2020cites this paper
• Relationships between the age of 25,445 men attending infertility clinics and sperm chromatin structure assay (SCSA®) defined sperm DNA and chromatin integrity.

  2020cites this paper
• The Sins of Our Forefathers: Paternal Impacts on De Novo Mutation Rate and Development.

  2020cites this paper
• Sperm DNA Fragmentation: A New Guideline for Clinicians

  2020influential citation
• Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?

  2020cites this paper
• Diagnosis and Treatment of Infertility in Men: AUA/ ASRM Guideline

  2020cites this paper
• Dolichandrone serrulata flower extract ameliorates male reproductive damages in type 2 diabetic rats

  2020cites this paper
• Influence of Exogenous Factors on the Maturation Levels of Spermatozoa Chromatin in Sub/Infertile Men Treated with Nutritional Supplement PAPA®

  2019cites this paper
• Evaluating the role of paternal factors in aetiology and prognosis of recurrent pregnancy loss: study protocol for a hospital-based multicentre case–control study and cohort study (REMI III project)

  2019cites this paper
• Evaluation of Sperm Chromatin Integrity Using Aniline Blue and Toluidine Blue Staining in Infertile and Normozoospermic Men

  2019cites this paper
• DNA damaging effect of paclitaxel in the epididymal sperms as a chemotherapeutic agent and possible remedies to prevent this effect: A study on reproductive potential of male cancer patients of reproductive age.

  2019cites this paper
• Cytochemical tests to investigate sperm DNA damage: Assessment and review

  2019cites this paper
• Effects of different doses of tamoxifen on the sperm parameters and chromatin quality in mice: An experimental model

  2019cites this paper
• Sperm DNA Damage Before and After Kidney Transplantation

  2019cites this paper
• Effects of Tamoxifen on DNA Integrity in Mice

  2019cites this paper
• Effects of Levofloxacin on Male Reproductive System Parameters and Sperm DNA Normality in Rats

  2018cites this paper
• Investigation on the origin of sperm morphological defects: oxidative attacks, chromatin immaturity, and DNA fragmentation

  2018cites this paper
• The effect of human sperm chromatin maturity on ICSI outcomes

  2018cites this paper
• Sperm aneuploidy and DNA fragmentation in unexplained recurrent pregnancy loss: a multicenter case-control study

  2018cites this paper
• Sperm chromatin and DNA integrity, methyltransferase mRNA levels, and global DNA methylation in oligoasthenoteratozoospermia

  2018cites this paper
• Sperm chromatin quality and DNA integrity in partial versus total globozoospermia

  2018cites this paper
• Use of testicular sperm in nonazoospermic males.

  2018cites this paper
• The Effect of Vitamin E and Selenium on Sperm Chromatin Quality in Couples with Recurrent Miscarriage

  2018cites this paper
• Assessment of Sperm Morphology, Chromatin Integrity, and Catsper Genes Expression in Hypothyroid Mice

  2018cites this paper
• Frequency of CYP1A1 Gene Polymorphisms in Infertile Men with Non-obstructive Azoospermia

  2018cites this paper
• Zingiber Officinale Water Extract Effect on Sperm of Alloxan-Induced Diabetic Mice: an In Vitro Examination of Sperm DNA Fragmentation, Fertilization and Embryonic Development Outcomes

  2018cites this paper
• EFFECTS OF CIPROFLOXACIN ON MALE FERTILITY PARAMETERS AND SPERM DNA INTEGRITY IN RATS

  2018cites this paper
• Administration of high dose of methamphetamine has detrimental effects on sperm parameters and DNA integrity in mice

  2017cites this paper
• Evaluating Damages to Sperm DNA

  2017cites this paper
• Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study

  2017cites this paper
• Assessment of human sperm DNA integrity using two cytochemical tests: Acridine orange test and toluidine blue assay

  2017influential citation
• Testicular toxicity and sperm quality following copper exposure in Wistar albino rats: ameliorative potentials of L-carnitine

  2017cites this paper
• Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity.

  2017cites this paper
• Our first experience with the comet assay in the study of sperm DNA integrity

  2016cites this paper
• The impact of cigarettes smoking on DNA integrity and sperm protein (histone, transistion protein and protamines) alterations

  2016cites this paper
• Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions

  2016cites this paper
• The effect of heracleum persicum (Golpar) oil and alcoholic extracts on sperm parameters and chromatin quality in mice

  2016cites this paper
• Effect of metformin therapy and dietary supplements on semen parameters in hyperinsulinaemic males

  2015influential citation
• MSOME and Sperm Chromatin Status

  2015cites this paper
• Sperm Vacuoles: Origin and Implications

  2015cites this paper
• Further evidence supporting the Diff-Quik stain for sperm chromatin integrity testing.

  2015cites this paper
• Investigation of the association between the outcomes of sperm chromatin condensation and decondensation tests, and assisted reproduction techniques

  2015cites this paper
• A Simple Sperm DNA Toroid Integrity Test and Risk of Miscarriage

  2015cites this paper
• Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study.

  2015cites this paper
• Effects of acrylamide on sperm parameters, chromatin quality, and the level of blood testosterone in mice

  2014influential citation
• The Role of some Medicinal Plants in Reducing Sperm DNA Damage of Male Mice

  2014cites this paper
• High-magnification selection of spermatozoa prior to oocyte injection: confirmed and potential indications.

  2014cites this paper
• Taurine reverses endosulfan-induced oxidative stress and apoptosis in adult rat testis.

  2014cites this paper
• CHD5 is required for spermiogenesis and chromatin condensation.

  2014cites this paper
• Vitamin C attenuates detrimental effects of diabetes mellitus on sperm parameters, chromatin quality and rate of apoptosis in mice.

  2014cites this paper
• The utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis

  2013influential citation
• Assessment of Chromatin Maturity in Human Spermatozoa: Useful Aniline Blue Assay for Routine Diagnosis of Male Infertility

  2013cites this paper
• Seminal bacterial contaminations: Probable factor in unexplained recurrent pregnancy loss

  2013influential citation
• Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure.

  2013cites this paper
• Effects of experimentally-induced diabetes on sperm parameters and chromatin quality in mice

  2013influential citation
• Sperm chromatin condensation, DNA integrity, and apoptosis in men with spinal cord injury

  2013cites this paper
• The impact of semen quality, occupational exposure to environmental factors and lifestyle on recurrent pregnancy loss

  2013cites this paper
• Embryology - Updates and Highlights on Classic Topics

  2012cites this paper
• The Actors of Human Implantation: Gametes, Embryo, Endometrium

  2012cites this paper
• The actors of human implantation: gametes, embryo and endometrium

  2012cites this paper
• Cytochemical Tests for Sperm Chromatin Maturity

  2011cites this paper