Polyphosphoinositides Inhibit the Interaction of Vinculin with Actin Filaments*

Binding of vinculin to adhesion plaque proteins is restricted by an intramolecular association of vinculin’s head and tail regions. Results of previous work suggest that polyphosphoinositides disrupt this interaction and thereby promote binding of vinculin to both talin and actin. However, data presented here show that phosphatidylinositol 4,5-bisphosphate (PI4,5P2) inhibits the interaction of purified tail domain with F-actin. Upon re-examining the effect of PI4,5P2 on the actin and talin-binding activities of intact vinculin, we find that when the experimental design controls for the effect of magnesium on aggregation of PI4,5P2 micelles, polyphosphoinositides promote interactions with the talin-binding domain, but block interactions of the actin-binding domain. In contrast, if vinculin is trapped in an open confirmation by a peptide specific for the talin-binding domain of vinculin, actin binding is allowed. These results demonstrate that activation of the actin-binding activity of vinculin requires steps other than or in addition to the binding of PI4,5P2.

• Publication year

  1999

• Venue

  Journal of Biological Chemistry

• Publication date

  1999-06-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/jbc.274.26.18414PMID 10373448
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• FEBS Lett

  2000cited by this paper
• The interaction of the cell-contact proteins VASP and vinculin is regulated by phosphatidylinositol-4,5-bisphosphate.

  1998cited by this paper
• Vinculin knockout results in heart and brain defects during embryonic development.

  1998cited by this paper
• A conserved motif in the tail domain of vinculin mediates association with and insertion into acidic phospholipid bilayers.

  1998cited by this paper
• Rescue of the mutant phenotype by reexpression of full-length vinculin in null F9 cells; effects on cell locomotion by domain deleted vinculin.

  1998cited by this paper
• Isolation of peptides from phage-displayed random peptide libraries that interact with the talin-binding domain of vinculin.

  1997cited by this paper
• Characterization of two F-actin-binding and oligomerization sites in the cell-contact protein vinculin.

  1997cited by this paper
• The crystal structure of plasma gelsolin: implications for actin severing, capping, and nucleation.

  1997cited by this paper
• The structure of divalent cation-induced aggregates of PIP2 and their alteration by gelsolin and tau.

  1997cited by this paper
• Intramolecular interactions regulate serine/threonine phosphorylation of vinculin

  1996cited by this paper
• Regulation of vinculin binding to talin and actin by phosphatidyl-inositol-4-5-bisphosphate

  1996cited by this paper
• Development

  1996cited by this paper
• The ultrastructure of chicken gizzard vinculin as visualized by high-resolution electron microscopy.

  1996cited by this paper
• Acidic phospholipids inhibit the intramolecular association between the N- and C-terminal regions of vinculin, exposing actin-binding and protein kinase C phosphorylation sites.

  1996cited by this paper
• Tertiary structure of destrin and structural similarity between two actin-regulating protein families.

  1996cited by this paper
• Vinculin, talin and focal adhesions

  1996cited by this paper
• Focal adhesion formation by F9 embryonal carcinoma cells after vinculin gene disruption.

  1995cited by this paper
• Targeted disruption of vinculin genes in F9 and embryonic stem cells changes cell morphology, adhesion, and locomotion.

  1995cited by this paper
• F-actin binding site masked by the intramolecular association of vinculin head and tail domains

  1995cited by this paper
• Interaction of the 47-kDa talin fragment and the 32-kDa vinculin fragment with acidic phospholipids: a computer analysis.

  1995cited by this paper
• The carboxy-terminal tail domain of vinculin contains a cryptic binding site for acidic phospholipids.

  1995cited by this paper
• Phosphoinositides and calcium as regulators of cellular actin assembly and disassembly.

  1994cited by this paper
• An intramolecular association between the head and tail domains of vinculin modulates talin binding.

  1994cited by this paper
• Structure of actin binding proteins: insights about function at atomic resolution.

  1994cited by this paper
• Adhesion to fibronectin stimulates inositol lipid synthesis and enhances PDGF-induced inositol lipid breakdown

  1993cited by this paper
• Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage-dependent growth of 3T3 cells

  1993cited by this paper
• Overexpression of vinculin suppresses cell motility in BALB/c 3T3 cells.

  1992cited by this paper
• Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA

  1992cited by this paper
• Vinculin is essential for muscle function in the nematode

  1991cited by this paper
• Metabolic radiolabeling techniques for identification of prenylated and fatty acylated proteins

  1990cited by this paper
• Purification and characterization of an 85 kDa talin-binding fragment of vinculin.

  1990cited by this paper
• cDNA-derived sequence of chicken embryo vinculin.

  1988cited by this paper
• Measurement of rate constants for actin filament elongation in solution.

  1983cited by this paper
• Identification of a factor in conventional muscle actin preparations which inhibits actin filament self-association.

  1980cited by this paper
• Vinculin, an intracellular protein localized at specialized sites where microfilament bundles terminate at cell membranes.

  1980cited by this paper
• A rapid purification of alpha-actinin, filamin, and a 130,000-dalton protein from smooth muscle.

  1980cited by this paper
• The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

  1971cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• ASSAY OF INORGANIC PHOSPHATE, TOTAL PHOSPHATE AND PHOSPHATASE

  1966cited by this paper
• Methods : A Companion to Methods in Enzymology

  year unknowncited by this paper

• Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin

  2020cites this paper
• Bio-Instructive Cues in Scaffolds for Musculoskeletal Tissue Engineering and Regenerative Medicine

  2017cites this paper
• Binding of Vinculin to Lipid Membranes in Its Inhibited and Activated States.

  2016cites this paper
• Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites*

  2016influential citation
• Phosphoinositide Binding Inhibits Actin Crosslinking and Polymerization by Palladin.

  2016cites this paper
• Role of vinculin in cellular mechanotransduction

  2016cites this paper
• Mechanosensitive components of integrin adhesions: Role of vinculin

  2016cites this paper
• Lipid-directed vinculin dimerization.

  2015cites this paper
• Dissertation Metavinculin

  2015cites this paper
• Essential role of class II PI3K-C2α in platelet membrane morphology.

  2015influential citation
• Protein Kinase C, Focal Adhesions and the Regulation of Cell Migration

  2014cites this paper
• Lipid binding promotes oligomerization and focal adhesion activity of vinculin

  2014cites this paper
• USING VINCULIN MUTANTS TO ASCERTAIN THE ROLE OF VINCULIN IN CELLULAR MOTILITY AND MECHANOTRANSDUCTION

  2014influential citation
• Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties.

  2014cites this paper
• Characterizing the Role of Actin Binding, Bundling, and Tyrosine Phosphorylation in Modulating Vinculin Function

  2013influential citation
• Vinculin and metavinculin: Oligomerization and interactions with F‐actin

  2013cites this paper
• Integrating actin dynamics, mechanotransduction and integrin activation: the multiple functions of actin binding proteins in focal adhesions.

  2013cites this paper
• Dimer asymmetry defines α-catenin interactions

  2013cites this paper
• Vinculin, cadherin mechanotransduction and homeostasis of cell–cell junctions

  2012cites this paper
• Integrin-Linked Kinase, ECM Composition, and Substrate Rigidity Regulate Focal Adhesion - Actin Coupling, Modulating Survival, Proliferation and Migration: Towards a Biophysical Cancer Biomarker

  2012cites this paper
• Effect of phosphorylation of phosphatidylinositol on myelin basic protein-mediated binding of actin filaments to lipid bilayers in vitro.

  2012cites this paper
• Monomeric and dimeric conformation of the vinculin tail five-helix bundle in solution studied by EPR spectroscopy.

  2011cites this paper
• Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis

  2011cites this paper
• Membrane androgen receptor activation triggers pro-apoptotic responses in vitro and in vivo and blocks migration in colon cancer

  2011cites this paper
• The Biomechanical Properties of 3d Extracellular Matrices and Embedded Cells Regulate the Invasiveness of Cancer Cells

  2011cites this paper
• Role of Abl and Src family kinases in actin-cytoskeletal rearrangements induced by the Helicobacter pylori CagA protein.

  2011cites this paper
• New insights into vinculin function and regulation.

  2011cites this paper
• Vinculin, an adapter protein in control of cell adhesion signalling

  2011cites this paper
• The Vinculin-ΔIn20/21 Mouse: Characteristics of a Constitutive, Actin-Binding Deficient Splice Variant of Vinculin

  2010cites this paper
• Rac controls PIP5K localisation and PtdIns(4,5)P2 synthesis, which modulates vinculin localisation and neurite dynamics

  2010cites this paper
• Regulation of the actin cytoskeleton-plasma membrane interplay by phosphoinositides.

  2010cites this paper
• Vinculin Is a Dually Regulated Actin Filament Barbed End-capping and Side-binding Protein

  2010cites this paper
• Osteoblasts response to microstructured and nanostructured polyimide film, processed by the use of silica bead microlenses.

  2010cites this paper
• Phosphoinositides and actin cytoskeletal rearrangement

  2010cites this paper
• The Cytoskeletal Connection: Understanding Adaptor Proteins

  2010cites this paper
• Correlation between the interaction of the vinculin tail domain with lipid membranes, its phosphorylation and cell mechanical behaviour

  2010influential citation
• Anchorage of vinculin to lipid membranes influences cell mechanical properties.

  2009cites this paper
• The Role of Vinculin in the Regulation of the Mechanical Properties of Cells

  2009cites this paper
• Internalization of fibronectin-binding S. aureus into mammalian cells via integrins and membrane microdomains

  2009cites this paper
• Lipid Binding to the Tail Domain of Vinculin

  2009cites this paper
• Focal Adhesions : New Angles on an Old Structure

  2009cites this paper
• Charakterisierung der Rekrutierungsdomäne der kleinen Rho-GTPasen RhoA und RhoC

  2009cites this paper
• Vinculin Nucleates Actin Polymerization and Modifies Actin Filament Structure*

  2009cites this paper
• Direct evidence of vinculin tail-lipid membrane interaction in beta-sheet conformation.

  2008cites this paper
• The role of pH, self-association, and lipid binding in vinculin tail structure and function

  2008cites this paper
• Regulation of actin assembly associated with protrusion and adhesion in cell migration.

  2008cites this paper
• The Helicobacter pylori CagA protein disrupts matrix adhesion of gastric epithelial cells by dephosphorylation of vinculin

  2007cites this paper
• Three-dimensional structure of vinculin bound to actin filaments.

  2006cites this paper
• Role of vinculin in regulating focal adhesion turnover.

  2006cites this paper
• The structure and regulation of vinculin.

  2006cites this paper
• The vinculin binding sites of talin and alpha-actinin are sufficient to activate vinculin.

  2006cites this paper
• Studies relating to molecular mechanism of CD44 augmented macrophage phagocytosis of apoptotic neutrophils

  2006cites this paper
• The Vinculin Binding Sites of Talin and α-Actinin Are Sufficient to Activate Vinculin*

  2006cites this paper
• Coincidence of Actin Filaments and Talin Is Required to Activate Vinculin*

  2006cites this paper
• Two Distinct Head-Tail Interfaces Cooperate to Suppress Activation of Vinculin by Talin*

  2005cites this paper
• Assembly and signaling of adhesion complexes.

  2005cites this paper
• Integrin-actin interactions

  2005cites this paper
• Smooth muscle alpha-actinin interaction with smitin.

  2005cites this paper
• Vinculin acts as a sensor in lipid regulation of adhesion-site turnover

  2005cites this paper
• Molecular basis of ataxia telangiectasia and related diseases

  2005cites this paper
• On the lipid mediated regulation of the cell adhesion protein vinculin

  2005cites this paper
• Smooth muscle α-actinin interaction with smitin

  2005cites this paper
• Structural basis for vinculin activation at sites of cell adhesion

  2004cites this paper
• Cytoskeletal regulation: rich in lipids

  2004cites this paper
• Dealing with mechanics: mechanisms of force transduction in cells.

  2004cites this paper
• Low-populated folding intermediates of Fyn SH3 characterized by relaxation dispersion NMR

  2004cites this paper
• The phosphorylation of vinculin on tyrosine residues 100 and 1065, mediated by SRC kinases, affects cell spreading.

  2004influential citation
• Vinculin is proteolyzed by calpain during platelet aggregation: 95 kDa cleavage fragment associates with the platelet cytoskeleton.

  2004cites this paper
• Die Invasion von Epithelzellen durch E. coli Shigella

  2004influential citation
• Phosphoinositides in Subcellular Targeting and Enzyme Activation

  2004cites this paper
• Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion.

  2004cites this paper
• Regulation of the actin cytoskeleton by PI(4,5)P2 and PI(3,4,5)P3.

  2004cites this paper
• Mitogenic signal transduction by integrin- and growth factor receptor-mediated pathways.

  2004cites this paper
• Vinculin modulation of paxillin–FAK interactions regulates ERK to control survival and motility

  2004cites this paper
• Talin: an emerging focal point of adhesion dynamics.

  2004cites this paper
• Cell biology: How to build a cell junction

  2004cites this paper
• CHAPTER 156 – Phosphoinositides and Actin Cytoskeletal Rearrangement

  2003cites this paper
• Phosphoinositide regulation of the actin cytoskeleton.

  2003cites this paper
• Understanding the role of the cytoskeleton in the complex regulation of the endothelial repair.

  2003cites this paper
• The integrin–actin connection, an eternal love affair

  2003cites this paper
• Integrin signaling to the actin cytoskeleton.

  2003cites this paper
• Phosphoinositide Binding Inhibits α-Actinin Bundling Activity*

  2003cites this paper
• Phosphoinositide binding inhibits alpha-actinin bundling activity.

  2003cites this paper
• A Lipid-regulated Docking Site on Vinculin for Protein Kinase C*

  2002cites this paper
• Regulation of Inositol Phospholipid Binding and Signaling through Syndecan-4*

  2002cites this paper
• The molecular basis for the autoregulation of calponin by isoform-specific C-terminal tail sequences.

  2002cites this paper
• The Extracellular Matrix in Neural Development, Plasticity, and Regeneration

  2002cites this paper
• Type Iγ phosphatidylinositol phosphate kinase targets and regulates focal adhesions

  2002cites this paper
• Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells

  2002cites this paper
• Vinexin, CAP/ponsin, ArgBP2: a novel adaptor protein family regulating cytoskeletal organization and signal transduction.

  2002cites this paper
• Microtubule-actin interactions may regulate endothelial integrity and repair.

  2002cites this paper
• The cytoplasmic face of cell contact sites.

  2002cites this paper
• Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions.

  2002cites this paper
• Recruitment of the Arp2/3 complex to vinculin

  2002cites this paper
• Further characterization of the interaction between the cytoskeletal proteins talin and vinculin.

  2002cites this paper
• Phosphatidylinositol-dependent actin filament binding by the SWI/SNF-like BAF chromatin remodeling complex

  2002cites this paper
• Molecular interactions of talin and actin: a study on the specific domains of talin involved in the interactions

  2002cites this paper
• Signaling in the Motility Responses of the Human Neutrophil

  2002cites this paper
• Tara, a novel F-actin binding protein, associates with the Trio guanine nucleotide exchange factor and regulates actin cytoskeletal organization.

  2001cites this paper
• Molecular motors involved in T cell receptor clusterings

  2001cites this paper