The polyphagous agromyzid fly, Liriomyza trifolii, is a significant and important insect pest of ornamental and vegetable crops worldwide. The adaptation of insects to different environments is facilitated by heat shock proteins (HSPs), which play an important role in acclimation to thermal stress. In this study, we cloned and characterized five HSP-encoding genes of L. trifolii (Lthsp20, Lthsp40, Lthsp60, Lthsp70, and Lthsp90) and monitored their expression under different thermal stresses using real-time quantitative PCR. Pupae of L. trifolii were exposed to 19 different temperatures ranging from -20 to 45°C. The results revealed that Lthsp20, Lthsp40, Lthsp70 and Lthsp90 were significantly upregulated in response to both heat and cold stress, while Lthsp60 was induced only by heat temperatures. The temperatures of the onset (Ton) and maximal (Tmax) expression of the five Lthsps were also determined and compared with published Ton and Tmax values of homologous genes in L. sativae and L. huidobrensis. Although L. trifolii occurs primarily in southern China, it has cold tolerance comparable with the other two Liriomyza species. Based on the heat shock proteins expression patterns, L. trifolii has the capacity to tolerate extreme temperatures and the potential to disseminate to northern regions of China.
Cloning and expression of genes encoding heat shock proteins in Liriomyza trifolii and comparison with two congener leafminer species
Ya-Wen Chang,Jing-yun Chen,M. Lu,Yuan Gao,Zi-Hua Tian,Weirong Gong,Chang-Sheng Dong,Yu-Zhou Du
Published 2017 in PLoS ONE
ABSTRACT
PUBLICATION RECORD
- Publication year
2017
- Venue
PLoS ONE
- Publication date
2017-07-20
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- five hsp-encoding genes
The set of five heat shock protein genes cloned and characterized in this work, including Lthsp20, Lthsp40, Lthsp60, Lthsp70, and Lthsp90.
Aliases: Lthsp20, Lthsp40, Lthsp60, Lthsp70, Lthsp90, Lthsps
- heat and cold stress response
An expression pattern in which transcript levels increase under both hot and cold temperature exposure.
Aliases: heat- and cold-induced response
- heat-only stress response
An expression pattern in which transcript levels increase under heat exposure but not under cold exposure.
Aliases: heat-induced only response
- liriomyza trifolii
A polyphagous agromyzid leafminer species used as the focal insect in the gene-cloning and expression experiments.
Aliases: L. trifolii, leafminer fly
- northward dissemination potential
The inferred capacity of the species to spread into northern regions based on its temperature tolerance profile.
Aliases: potential to disseminate to northern regions of China
- real-time quantitative pcr
A transcript quantification method used here to measure gene expression under different temperature treatments.
Aliases: RT-qPCR, qPCR
- thermal stress
The range of temperature treatments, from cold to heat, applied to pupae during the expression experiments.
Aliases: temperature stress, temperature treatments
- ton and tmax expression
The onset temperature and maximal-expression temperature used to summarize when each gene begins responding and when its expression peaks.
Aliases: Ton, Tmax
REFERENCES
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