A novel family of divergent seven-transmembrane proteins: candidate odorant receptors in Drosophila.

Although insects have proven to be valuable models for exploring the function, organization, and development of the olfactory system, the receptor molecules that bind odors have not been identified in any insect. We have developed a novel search algorithm, used it to search the Drosophila genomic sequence database, and identified a large multigene family encoding seven transmembrane domain proteins that are expressed in olfactory organs. We show that expression is restricted to subsets of olfactory receptor neurons (ORNs) for a number of these genes. Different members of the family initiate expression at different times during antennal development. Some of the genes are not expressed in a mutant of the Acj6 POU-domain transcription factor, a mutant in which a subset of ORNs show abnormal odorant specificities.

• Publication year

  1999

• Venue

  Neuron

• Publication date

  1999-02-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0896-6273(00)81093-4PMID 10069338
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Development

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  1991influential reference
• Basic local alignment search tool.

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• Odorant-sensitive phospholipase C in insect antennae.

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