Time- and Ventricular-Specific Expression Profiles of Genes Encoding Z-Disk Proteins in Pressure Overload Model of Left Ventricular Hypertrophy

Mechanotransduction is an essential mechanism of transforming external mechanical stimulus to biochemical response. In cardiomyocytes mechanotransduction plays an important role in contraction, stretch sensing and homeostasis regulation. One of the major mechanosensitive area in cardiomyocytes, the Z-disk, consists of numbers of structural and signaling proteins, that may undergo conformational or gene expression changes under pathological stress conditions. In present study we examined a rat model of pressure overload cardiac hypertrophy validated by echocardiographic and histopathological examinations. We revealed, that during hypertrophy progression expression of several genes encoding Z-disk proteins (Actn2, Ldb3, Cmya5, Nebl) is different at early and late points of cardiac remodeling. Moreover, expression patterns of several genes are opposite in myocardium of overloaded left ventricle and hemodynamically unaffected right ventricle, and expression profiles in interventricular septum are more similar to right ventricle. Additionally, we revealed inconsistencies between mRNA and protein level changes of Actn2, one of the major structural Z-disk element. All these findings point out, that investigated Z-disk proteins participate in pathological stress adaptation through undergoing the gene expression changes, and suggest the novel important role of hypertrophic response modulation during different stages of cardiac remodeling.

• Publication year

  2019

• Venue

  Frontiers in Genetics

• Publication date

  2019-01-07

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3389/fgene.2018.00684PMID 30666270PMCID 6330284
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dysregulated autophagy in restrictive cardiomyopathy due to Pro209Leu mutation in BAG3.

  2018cited by this paper
• Genetic epidemiology of titin-truncating variants in the etiology of dilated cardiomyopathy

  2017cited by this paper
• Association between mutation status and left ventricular reverse remodelling in dilated cardiomyopathy

  2017cited by this paper
• The sarcomeric cytoskeleton: from molecules to motion

  2016cited by this paper
• Rapamycin Inhibits Cardiac Hypertrophy by Promoting Autophagy via the MEK/ERK/Beclin-1 Pathway

  2016cited by this paper
• Genetic Spectrum of Idiopathic Restrictive Cardiomyopathy Uncovered by Next-Generation Sequencing

  2016cited by this paper
• Increases of desmin and α-actinin in mouse cardiac myofibrils as a response to diastolic dysfunction.

  2016cited by this paper
• Mechanotransduction in cardiac hypertrophy and failure.

  2015cited by this paper
• Guidelines for the use and interpretation of diagnostic methods in adult food allergy

  2015cited by this paper
• Involvement of BAG3 and HSPB7 loci in various etiologies of systolic heart failure: Results of a European collaboration assembling more than 2000 patients.

  2015cited by this paper
• Molecular genetics and pathogenesis of cardiomyopathy

  2015cited by this paper
• Recommendations for cardiac chamber quantification by echocardiography in adults: an update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging.

  2015cited by this paper
• Myocardial Mechanics in a Rat Model with Banding and Debanding of the Ascending Aorta

  2014cited by this paper
• Novel genotype–phenotype associations demonstrated by high-throughput sequencing in patients with hypertrophic cardiomyopathy

  2014cited by this paper
• Regulation of expression of atrial and brain natriuretic peptide, biomarkers for heart development and disease.

  2013cited by this paper
• Pressure‐Overload Hypertrophy of the Developing Heart Reveals Activation of Divergent Gene and Protein Pathways in the Left and Right Ventricular Myocardium

  2013cited by this paper
• The Sarcomeric Z-Disc and Z-Discopathies

  2011cited by this paper
• Identification of right heart-enriched genes in a murine model of chronic outflow tract obstruction.

  2010cited by this paper
• Mechanotransduction gone awry

  2009cited by this paper
• The vertebrate muscle Z-disc: sarcomere anchor for structure and signalling

  2009cited by this paper
• Identification of interventricular septum precursor cells in the mouse embryo.

  2007cited by this paper
• Cardiac dysfunction and heart failure are associated with abnormalities in the subcellular distribution and amounts of oligomeric muscle LIM protein.

  2007cited by this paper
• Cardiac autophagy is a maladaptive response to hemodynamic stress.

  2007cited by this paper
• Right ventricular function and failure: report of a National Heart, Lung, and Blood Institute working group on cellular and molecular mechanisms of right heart failure.

  2006cited by this paper
• The sarcomeric Z-disc: a nodal point in signalling and disease

  2006cited by this paper
• Early and transient gene expression changes in pressure overload-induced cardiac hypertrophy in mice.

  2006cited by this paper
• Mechanical stress-strain sensors embedded in cardiac cytoskeleton: Z disk, titin, and associated structures.

  2006cited by this paper
• At the crossroads of myocardial signaling: the role of Z-discs in intracellular signaling and cardiac function.

  2004cited by this paper
• Right Ventricular Myocardium Derives From the Anterior Heart Field

  2004cited by this paper
• Oriented clonal cell growth in the developing mouse myocardium underlies cardiac morphogenesis

  2004cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Differentiation- and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein

  2001cited by this paper
• Echocardiographic assessment of left ventricular hypertrophy: comparison to necropsy findings.

  1986cited by this paper
• Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 2 DD C T Method

  year unknowncited by this paper
• A genome-wide association study identifies two loci associated with heart failure due to dilated cardiomyopathy

  year unknowncited by this paper

• Strategic justification of integrated resource planning tools in organizations

  2024cites this paper
• CMYA5 is a novel interaction partner of FHL2 in cardiac myocytes

  2022cites this paper
• Emerging Role of TRIM Family Proteins in Cardiovascular Disease

  2020cites this paper
• ZNF354C is a transcriptional repressor that inhibits endothelial angiogenic sprouting

  2020cites this paper
• Comparative analysis of the transcriptomes of EDL, psoas, and soleus muscles from mice

  2020cites this paper