Role of non-coding RNA in epigenetic inheritance of ribosomal RNA gene silencing

Faithful propagation of specific chromatin states requires re-establishment of epigenetic marks after every cell division. How the original epigenetic signature is inherited after disruption during DNA replication is still poorly understood. Here we show that the poly(ADP-ribose)-polymerase-1 (PARP1/ARTD1) is implicated in the maintenance of silent rDNA chromatin during cell division. We demonstrate that PARP1 associates with TIP5, a subunit of the NoRC complex, via the noncoding pRNA and binds to silent rRNA genes after their replication in mid-late Sphase. PARP1 represses rRNA transcription and is implicated in the formation of silent rDNA chromatin. Silent rDNA chromatin is a specific substrate for ADPribosylation and the enzymatic activity of PARP1 is necessary to establish rDNA silencing. The data unravel a novel function of PARP1 and ADP-ribosylation that serves to inherit silent chromatin structures, shedding light on how epigenetic marks are transmitted during each cell cycle.

• Publication year

  2012

• Venue

  Unknown venue

• Publication date

  2012-12-01

• Fields of study

  Biology

• Identifiers
  DOI 10.5167/uzh-57046
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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