Nitroreductase-Activatable Morpholino Oligonucleotides for in Vivo Gene Silencing

Phosphorodiamidate morpholino oligonucleotides are widely used to interrogate gene function in whole organisms, and light-activatable derivatives can reveal spatial and temporal differences in gene activity. We describe here a new class of caged morpholino oligonucleotides that can be activated by the bacterial nitroreductase NfsB. We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting.

• Publication year

  2014

• Venue

  ACS Chemical Biology

• Publication date

  2014-07-28

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/cb500429uPMID 25069083PMCID 4168795
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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