Thermostable Lipase of Bacillus stearothermophilus: High-level Production, Purification, and Calcium-dependent Thermostability

No abstract is available for this paper.

• Publication year

  2000

• Venue

  Bioscience, biotechnology and biochemistry

• Publication date

  2000-01-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1271/bbb.64.280PMID 10737182
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Identification of a calcium binding site in Staphylococcus hyicus lipase: generation of calcium-independent variants.

  1999cited by this paper
• A Cold-Adapted Lipase of an Alaskan Psychrotroph,Pseudomonas sp. Strain B11-1: Gene Cloning and Enzyme Purification and Characterization

  1998cited by this paper
• High-level expression of the thermoalkalophilic lipase from Bacillus thermocatenulatus in Escherichia coli

  1998cited by this paper
• Gene cloning and characterization of thermostable lipase from Bacillus stearothermophilus L1.

  1998cited by this paper
• Determination of the kinetic parameters during continuous cultivation of the lipase-producing thermophile Bacillus sp. IHI-91 on olive oil

  1997cited by this paper
• Thermoalkalophilic lipase of Bacillus thermocatenulatus large-scale production, purification and properties: aggregation behaviour and its effect on activity.

  1997cited by this paper
• Purification and partial characterization of thermostable carboxyl esterase from Bacillus stearothermophilus L1

  1997cited by this paper
• Thermoalkalophilic lipase of Bacillus thermocatenulatus. I. molecular cloning, nucleotide sequence, purification and some properties.

  1996cited by this paper
• Bacterial lipases.

  1994cited by this paper
• The crystal structure of triacylglycerol lipase from Pseudomonas glumae reveals a partially redundant catalytic aspartate

  1993cited by this paper
• Extracellular lipase from Pseudomonas aeruginosa is an amphiphilic protein.

  1992cited by this paper
• Purification of lipases.

  1992cited by this paper
• Purification and characterization of a novel thermostable lipase from Pseudomonas cepacia.

  1992cited by this paper
• Purification and characterization of a novel thermostable lipase from Bacillus sp.

  1991cited by this paper
• Enzymatic catalysis in monophasic organic solvents

  1989cited by this paper
• [On the role of calcium ions during the hydrolysis of insoluble triglycerides by pancreatic lipase in the presence of bile salts].

  1968cited by this paper
• Action of some effectors on the hydrolysis of long-chain triglycerides by pancreatic lipase.

  1968cited by this paper
• Sur le role des ions calcium durant l'hydrolyse des triglycerides insolubles par la lipase pancreatique en presence de sels biliares

  1968cited by this paper
• Instrumental assay of microbial lipase at constant pH.

  1967cited by this paper

• Thermostable Bacterial Esterases From Lipase Family 1.5 Degrade Compostable Polyesters PBAT and PBSA

  2025cites this paper
• Enzyme loading in the support and medium composition during immobilization alter activity, specificity and stability of octyl agarose-immobilized Eversa Transform.

  2025cites this paper
• Insight into improved specificity and thermostability of Geobacillus zalihae T1 lipase by introducing novel molecular interactions via phenylalanin to cysteine substitution

  2025cites this paper
• Unraveling the potential of uninvestigated thermoalkaliphilic lipases by molecular docking and molecular dynamic simulation: an in silico characterization study

  2024influential citation
• Do lipases and amylases of an endangered Indian carp Hypselobarbus pulchellus play a role in visceral fat dynamics; evidence of in vitro macromolecular interactions.

  2024cites this paper
• Production, purification, properties and current perspectives for modification and application of microbial lipases

  2024cites this paper
• Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization

  2024cites this paper
• Recombinant Expression and Characterization of a Novel Thermo-Alkaline Lipase with Increased Solvent Stability from the Antarctic Thermophilic Bacterium Geobacillus sp. ID17

  2024cites this paper
• Isolation, Identification, and Genomic Analysis of a Bacillus safensis Lipid-Biodegrading Strain

  2023cites this paper
• The main Aflatoxin B1 degrading enzyme in Pseudomonas putida is thermostable lipase

  2022cites this paper
• Biochemical characterization, substrate and stereoselectivity of an outer surface putative α/β hydrolase from the pathogenic Leptospira.

  2022cites this paper
• Bakteriyel Kaynaklı Lipaz Geninin Pichia pastoris’te Klonlanması, Ekspresyonu ve Rekombinant Enzimin Saflaştırılması

  2022cites this paper
• A jacalin-related lectin domain-containing lipase from chestnut (Castanea crenata): Purification, characterization, and protein identification

  2022cites this paper
• Serratia sp. scl1: isolation of a novel thermostable lipase producing microorganism which holds industrial importance

  2022cites this paper
• Extremophilic lipases for industrial applications: A general review.

  2022cites this paper
• Kinetic and thermodynamic characterization of novel alkaline lipase from halotolerant Bacillus gibsonii

  2021cites this paper
• Purification of Bacillus licheniformis Lipase and its Application as an Additive in Detergent for Destaining

  2021cites this paper
• Thermostable lipases and their dynamics of improved enzymatic properties

  2021cites this paper
• Current perspectives for microbial lipases from extremophiles and metagenomics.

  2021cites this paper
• Increasing Hydrolytic Activity of Lipase on Palm Oil by PCR-Based Random Mutagenesis

  2020cites this paper
• Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling.

  2020cites this paper
• Ion-Pair Interaction and Hydrogen Bonds as Main Features of Protein Thermostability in Mutated T1 Recombinant Lipase Originating from Geobacillus zalihae

  2020cites this paper
• Lipolytic activity of viscera extract from three freshwater fish species in Phatthalung, Thailand: Comparative studies and potential use as dishwashing detergent additive

  2019cites this paper
• Production of Recombinant Microbial Thermostable Lipases

  2019cites this paper
• X-ray structure and characterization of a thermostable lipase from Geobacillus thermoleovorans.

  2019cites this paper
• Changes of Thermostability, Organic Solvent, and pH Stability in Geobacillus zalihae HT1 and Its Mutant by Calcium Ion

  2019influential citation
• Advanced biotechnology in biorefinery: a new insight into municipal waste management to the production of high-value products

  2018cites this paper
• Aqueous Methods for Extraction/Recovery of Macromolecules From Microorganisms of Atypical Environments: A Focus on Three Phase Partitioning

  2018cites this paper
• Investigating the structural properties of the active conformation BTL2 of a lipase from Geobacillus thermocatenulatus in toluene using molecular dynamic simulations and engineering BTL2 via in-silico mutation

  2018cites this paper
• Sequential Detection of Thermophilic Lipase and Protease by Zymography.

  2017cites this paper
• The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties

  2017cites this paper
• Newly isolated yeasts from Tunisian microhabitats: Lipid accumulation and fatty acid composition

  2017cites this paper
• ISOLATION AND ENZYMATIC CHARACTERIZATION OF STREPTOMYCES ISOLATES FROM WESTERN RAJASTHAN

  2017cites this paper
• An organic solvent‐stable lipase from a newly isolated Staphylococcus aureus ALA1 strain with potential for use as an industrial biocatalyst

  2016cites this paper
• Cloning, expression, purification and characterization of lipase from Bacillus licheniformis, isolated from hot spring of Himachal Pradesh, India

  2016cites this paper
• Understanding the activation mechanism of Thermomyces lanuginosus lipase using rational design and tryptophan‐induced fluorescence quenching

  2016cites this paper
• Microbial-processing of fruit and vegetable wastes for production of vital enzymes and organic acids: Biotechnology and scopes.

  2016cites this paper
• Purification and characterization of lipase from Burkholderia sp. EQ3 isolated from wastewater from a canned fish factory and its application for the synthesis of wax esters

  2015cites this paper
• In vivo functional expression of an extracellular Ca2+-independent Bacillus pumilus lipase in Bacillus subtilis WB800N

  2015cites this paper
• Molecular cloning and characterization of a thermostable lipase from deep-sea thermophile Geobacillus sp. EPT9

  2015cites this paper
• Purification and characterization of an extracellular cold‐adapted alkaline lipase produced by psychrotrophic bacterium Yersinia enterocolitica strain KM1

  2015cites this paper
• Recombinant Lipase Engineered with Amphipathic and Coiled-Coil Peptides

  2015cites this paper
• Structural insights into methanol-stable variants of lipase T6 from Geobacillus stearothermophilus

  2015cites this paper
• Optimization of Lipase Production by Thermo-Alkalophilic Bacillus sp. 8C

  2015cites this paper
• Thermostable Lipases : an Overview of Production , Purification and Characterization

  2015cites this paper
• Preparation of nano-magnetite impregnated mesocellular foam composite with a Cu ligand for His-tagged enzyme immobilization

  2015cites this paper
• Enhancement of sequential zymography technique for the detection of thermophilic lipases and proteases

  2014cites this paper
• Molecular cloning and characterization of a thermostable lipase from deep-sea thermophile Geobacillus sp. EPT9

  2014cites this paper
• Structural basis for the Ca2+-enhanced thermostability and activity of PET-degrading cutinase-like enzyme from Saccharomonospora viridis AHK190

  2014cites this paper
• Purification and Characterization of Highly Alkaline Lipase from Bacillus licheniformis MTCC 2465: and Study of its Detergent Compatibility and Applicability

  2014cites this paper
• Functional expression of a novel alkaline-adapted lipase of Bacillus amyloliquefaciens from stinky tofu brine and development of immobilized enzyme for biodiesel production

  2014cites this paper
• A new thermostable and organic solvent-tolerant lipase from Aneurinibacillus thermoaerophilus strain HZ

  2013cites this paper
• Biocatalysis Through Thermostable Lipases: Adding Flavor to Chemistry

  2013cites this paper
• Optimization of extracellular thermophilic highly alkaline lipase from thermophilic bacillus sp isolated from hotspring of Arunachal Pradesh, India

  2012cites this paper
• Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1–7 Isolated from bohai bay in china for detergent formulation

  2012cites this paper
• Efficacy of intraumbilical vein ( IUV ) injection of oxytocin in active management of the third stage of labor

  2012cites this paper
• Bioremediation of Khormah Slaughter House Wastes by Production of Thermoalkalistable Lipase for Application in Leather Industries

  2012cites this paper
• Purification and Characterisation of an F16L Mutant of a Thermostable Lipase

  2012cites this paper
• Novel Metagenome-Derived, Cold-Adapted Alkaline Phospholipase with Superior Lipase Activity as an Intermediate between Phospholipase and Lipase

  2012cites this paper
• Lipase activity in Thermus thermophilus HB8: Purification and characterization of the extracellular enzyme

  2012cites this paper
• Lipase from Aeromonas caviae AU04: Isolation, purification and protein aggregation

  2012cites this paper
• Isolation of lipase producing thermophilic bacteria: optimization of production and reaction conditions for lipase from Geobacillus sp.

  2012cites this paper
• Enzymes used in detergents: Lipases

  2012cites this paper
• Catalytic properties and potential applications of Bacillus lipases

  2011cites this paper
• Fervidobacterium changbaicum Lip1: identification, cloning, and characterization of the thermophilic lipase as a new member of bacterial lipase family V

  2011cites this paper
• Production and characterization of lipase from Bacillus stearothermophilus

  2011cites this paper
• Growth effects on mixed culture of Dunaliella salina and Phaeodactylum tricornutum under different inoculation densities and nitrogen concentrations

  2011cites this paper
• Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119

  2011cites this paper
• Aktywność bakterii Bacillus subtilis BH w biodegradacji zaolejonej ziemi bielącej

  2011cites this paper
• Purification, characterization and thermal inactivation kinetics of a non-regioselective thermostable lipase from a genotypically identified extremophilic Bacillus subtilis NS 8.

  2011cites this paper
• Conformational plasticity of the calcium-binding pocket in the Burkholderia glumae lipase: remodeling induced by mutation of calcium coordinating residues.

  2011cites this paper
• Singlet oxygen's response to protein dynamics.

  2011cites this paper
• On the importance of the small domain in the thermostability of thermoalkalophilic lipases from l1 and t1: insights from molecular dynamics simulation.

  2010cites this paper
• Purification and Properties of a Lipase from Thermophilic Geobacillus stearothermophilus Strain-5

  2010cites this paper
• Cloning, expression, and biochemical characterization of a thermostable lipase from Geobacillus stearothermophilus JC

  2010cites this paper
• Cell kill pattern and acute toxicity studies of the aqueous fraction of the methanolic extract of parts of Parkia biglobosa

  2010cites this paper
• A unique thermostable and organic solvent tolerant lipase from newly isolated Aneurinibacillus thermoaerophilus strain HZ: physical factor studies

  2010influential citation
• Cloning and nucleotide sequence of a lipase gene from a soil isolate

  2009cites this paper
• Thermoactivity and effects of organic solvents on digestive lipase from hepatopancreas of the green crab

  2009cites this paper
• Highly efficient production of monoglycerides by the continuous removal of fatty acids from lipase-catalyzed oil hydrolysis

  2009cites this paper
• Enzymatic synthesis of banana flavour (isoamyl acetate) by Bacillus licheniformis S-86 esterase

  2009cites this paper
• Molecular Cloning and Characterization of a Lipase from an Indigenous Bacillus pumilus

  2009cites this paper
• Gene Cloning, Expression, and Characterization of the Geobacillus Thermoleovorans CCR11 Thermoalkaliphilic Lipase

  2009cites this paper
• Expanded bed adsorption of bromelain (E.C. 3.4.22.33) from Ananas comosus crude extract

  2009cites this paper
• Methods for detection and characterization of lipases: A comprehensive review.

  2009cites this paper
• Heterologe Expression von Enzymen aus Basidiomyceten

  2009cites this paper
• Production and Characterization of an Alkaline Thermostable Crude Lipase from an Isolated Strain of Bacillus cereus C7

  2009cites this paper
• Secretory expression and characterization of a highly Ca2+-activated thermostable L2 lipase.

  2009cites this paper
• Purification and characterization of extracellular lipase from a new strain: Pseudomonas aeruginosa SRT 9

  2009cites this paper
• Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis

  2009cites this paper
• Production of Thermostable Lipolytic Activity by Thermus Species

  2008cites this paper
• Thermal spring water enhances lipolytic activity in Thermus thermophilus HB27

  2008cites this paper
• Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors

  2007cites this paper
• Purification and Characterization of Two Highly Thermophilic Alkaline Lipases from Thermosyntropha lipolytica

  2007cites this paper
• Lipases from extremophiles and potential for industrial applications.

  2007cites this paper
• Selection of Lactobacillus sp. PSC101 that produces active dietary enzymes such as amylase, lipase, phytase and protease in pigs.

  2007cites this paper
• Functional expression of lipase A from Candida antarctica in Escherichia coli—A prerequisite for high-throughput screening and directed evolution

  2007cites this paper
• Purification and properties of enantioselective lipase from a newly isolated Bacillus cereus C71

  2007cites this paper
• Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm—a screening system for a frequently used biocatalyst

  2006cites this paper
• Isolation and characterization of lipase producing bacteria

  2006cites this paper