Evaluation of eugenol toxicity in bioassays with test-organisms

EnglishFish in both natural and farming conditions are exposed to stress of capture, handling, transport or treatment that provoke low zootechnical performance. Anesthetics like eugenol obtained from clove oil have been used strategically not only in freshwater but also in marine and estuarine fish in order to reduce the stress. Apart from the eugenol indication as anesthetic and its low toxicity for animals, its environment action is not clear. Bioassays or ecotoxicity tests with indicator organisms are used to evaluate the mode of action of the pollutants in the environment. The aim of this study was to test the acute toxicity of eugenol using the microcrustacean Daphnia magna and the bacterium Aliivibrio fischeri, and also its chronic toxicity for the algae Desmodesmus subspicatus. Eugenol in the concentrations of 50, 75 and 100mg L-1 were toxic to tested indicator organisms. Key words: Aliivibrio fischeri; Daphnia magna; Desmodesmus subspicatus; ecotoxicology portuguesOs peixes, seja em ambiente natural ou em piscicultura, sofrem efeito do estresse na captura, manejo, transporte ou tratamentos que provocam baixo desempenho zootecnico. Anestesicos, como o eugenol, obtido a partir do oleo de cravo, tem sido usado estrategicamente nao so em peixes de agua doce, mas tambem em peixes marinhos e estuarinos para reduzir o estresse. Apesar da indicacao de eugenol como anestesico em peixes e da sua baixa toxicidade para estes animais, nao se tem clareza da sua acao sobre o ambiente. Bioensaios ou testes de ecotoxicidade com organismos indicadores sao usados em para avaliar o modo de acao de poluentes no ambiente. O objetivo deste estudo foi testar a toxicidade aguda do eugenol utilizando o microcrustaceo Daphnia magna e a bacteria Aliivibrio fischeri e sua toxicidade cronica para alga Desmodesmus subspicatus. O eugenol, nas concentracoes de 50, 75 e 100mg L-1, foi toxico para organismos indicadores testados. Palavras-chave: Aliivibrio fischeri; Daphnia magna; Desmodesmus subspicatus; ecotoxicologia

• Publication year

  2017

• Venue

  Ciencia Rural

• Publication date

  2017-12-07

• Fields of study

  Not labeled

• Identifiers
  DOI 10.1590/0103-8478CR20170194
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• Acute toxicity was assessed with Daphnia magna and Aliivibrio fischeri, while chronic toxicity was assessed with Desmodesmus subspicatus.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• Eugenol at 50, 75, and 100 mg L-1 was toxic to the indicator organisms used in the assays.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review

• acute toxicity

  endpoint, toxicity endpoint

  Short-term toxic effects measured in the bioassays on the test organisms.

  Aliases: acute toxic effect

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• aliivibrio fischeri

  organism, test organism

  A bioluminescent bacterium used here as an indicator organism in the toxicity assays.

  Aliases: A. fischeri, Vibrio fischeri

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• chronic toxicity

  endpoint, toxicity endpoint

  Longer-term toxic effects measured in the algal assay.

  Aliases: chronic toxic effect

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• daphnia magna

  organism, test organism

  A microcrustacean used here as one of the indicator organisms in the toxicity assays.

  Aliases: D. magna

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• desmodesmus subspicatus

  organism, test organism

  A green alga used here as the organism for the chronic toxicity assay.

  Aliases: D. subspicatus

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review
• eugenol

  compound, analyte

  A clove-oil-derived compound tested here as the substance whose toxicity was evaluated in bioassays.

  Aliases: clove oil eugenol

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewq (76h6bfydm6) review

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