Suitability of chlorine dioxide as a tertiary treatment for municipal wastewater and use of reclaimed water for overhead irrigation of baby lettuce

L. T. Decol,F. López-Gálvez,P. Truchado,E. Tondo,M. Gil,A. Allende

Published 2019 in Food Control

ABSTRACT

Abstract Reclaimed wastewater used for agricultural irrigation should meet specific microbiological standards in order to prevent microbial contamination of the irrigated produce. The objective of this study was to evaluate the suitability of chlorine dioxide (ClO2) for the disinfection of secondary-treated municipal wastewater and its subsequent use for overhead irrigation in greenhouse production of baby lettuce. The impact of reclaimed water tertiary-treated with ClO2 on E. coli concentration, the presence of pathogenic bacteria, and the occurrence of chlorates as disinfection by-products was evaluated in water and in baby lettuce. E. coli was quantified using both conventional plating methods and a quantitative real time PCR (qPCR) method with propidium monoazide (PMA) pre-treatment to differentiate between viable and non-viable bacteria. Population density of cultivable E. coli was significantly lower (p

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