Selective separation and characterisation of dualACEandDPP‐IVinhibitory peptides from rainbow trout (Oncorhynchus mykiss) protein hydrolysates

Microwave pretreatment and hydrolysis were applied to rainbow trout (Oncorhynchus mykiss) by‐products to produce bioactive peptides with dual in vitro angiotensin‐I converting enzyme (ACE) and dipeptidyl‐peptidase IV (DPP‐IV) inhibitory activities. Peptides were fractionated using the single step electrodialysis with ultrafiltration membrane (EDUF). Concentration of cationic peptides (CP) increased in the recovery solution, reaching 125 μg mL⁻¹ after a 4‐h treatment with migration rate of 15.68 ± 2.98 g m⁻² h. CP fractions displayed ACE and DPP‐IV I inhibitory properties, with IC₅₀ values of 0.0036 mg mL⁻¹ and 1.23 mg mL⁻¹ respectively. The bioactivity was attributed to the low molecular weight peptides (300–500 Da) recovered. CP exhibited non‐competitive inhibition patterns for ACE and DPP‐IV, which were dose dependent. These results showed that bioactive peptides can successfully be separated from complex hydrolysate mixtures by EDUF. The fractionated peptides can serve as potential functional food ingredients or nutraceuticals for the management of hypertension and diabetes.

• Publication year

  2018

• Venue

  International Journal of Food Science & Technology

• Publication date

  2018-09-03

• Fields of study

  Biology, Chemistry, Environmental Science

• Identifiers
  DOI 10.1111/IJFS.13939
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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