Mode of action of 5-bromodeoxyuridine on mammalian cells in culture.

The effects of 5-bromouracil, an analogue of thymine, have been extensively studied in microbial systems. The incorporation of this analogue into the deoxyribonucleic acid of several strains of bacteria has been demonstrated (l-5). This phenomenon was shown to be associated with morphological changes caused by unbalanced growth (1,2,5), with decrease in viability (1, 5) and with the occurrence of 6-methylaminopurine as a structural part of DNA (2). The deoxyribonucleoside of 5-bromouracil, an analogue of thymidiie, has also been studied in bacterial systems and found to be both an inhibitor (6-8) and a growth factor (8). This compound has been shown to inhibit the incorporation of labeled formaldehyde (Q), formate, erotic acid, and thymidine (10) into DNA-thymine of mammalian tissues in vitro and of labeled formaldehyde into DNA-thymine of spleen and tumor t,issues in u-iuo (9). In the present study, the incorporation of 5-bromodeoxyuridine into DNA of HeLa cells was demonstrated and this phenomenon was shown to be associated with unbalanced growth and loss of viability of the cell. A preliminary report on these findings has been presented (11).

• Publication year

  1959

• Venue

  Journal of Biological Chemistry

• Publication date

  1959-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)69623-1PMID 14398911
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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