Cloning of the gp49B gene of the immunoglobulin superfamily and demonstration that one of its two products is an early-expressed mast cell surface protein originally described as gp49.

gp49 was originally defined as a 49-kDa surface glycoprotein preferentially expressed on mouse interleukin-3-dependent, bone marrow-derived mast cells, which are immature progenitor cells. A previously cloned cDNA (gp49A) indicated that gp49 was a member of the immunoglobulin (Ig) superfamily, and genomic DNA analysis indicated that two genes might encode a gp49 family. We have now characterized a 5.6-kilobase pair gene, gp49B, that encodes two novel gp49 cDNAs, gp49B1 and gp49B2. The two cDNAs are identical except that gp49B2 is missing exon 6, which encodes a predicted transmembrane domain. In contrast to gp49A, gp49B1 and gp49B2 have 32 additional amino acids at their C termini containing 4 of the 6 consensus amino acids of the antigen receptor homology 1 motif found on several signal-transducing members of the Ig superfamily. When COS-7 cells were transfected with either the gp49B1 or gp49B2 cDNA, only the gp49B1 transfectants bound the B23.1 monoclonal antibody that originally defined gp49. Reverse transcriptase-polymerase chain reaction analysis of the transfectants established that both transcripts were expressed, suggesting that the product of the gp49B2 transcript was not inserted in the plasma membrane. Thus, cloning of the gp49B gene has established the organization of one of the gp49 genes and provided evidence of alternate splicing of transcripts from that gene.

• Publication year

  1994

• Venue

  Journal of Biological Chemistry

• Publication date

  1994-03-18

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(17)37207-1PMID 8132564
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Cloning and transcript analysis of gp49B gene provide evidence of alternate splicing.

  Confidence 0.91

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review
• gp49B2 cDNA lacks exon 6, which encodes a predicted transmembrane domain, and only gp49B1 cDNA produces a product that binds the B23.1 monoclonal antibody.

  Confidence 0.94

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review
• gp49B gene encodes two novel cDNAs, gp49B1 cDNA and gp49B2 cDNA.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review

• alternate splicing

  RNA processing

  Production of different transcripts from the same gene by differential exon inclusion.

  Aliases: alternative splicing

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review
• b23.1 monoclonal antibody

  antibody

  A monoclonal antibody used as the original marker for gp49 surface protein.

  Aliases: B23.1

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review
• exon 6

  exon

  The sixth exon of the gp49B gene described in the transcript comparison.

  Aliases: gp49B exon 6

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review
• gp49b1 cdna

  cDNA

  One of the two cDNA isoforms encoded by gp49B and analyzed in the abstract.

  Aliases: gp49B1

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review
• gp49b2 cdna

  cDNA

  The second cDNA isoform encoded by gp49B and analyzed in the abstract.

  Aliases: gp49B2

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review
• gp49b gene

  gene

  A 5.6-kilobase gp49-family gene cloned and characterized in the abstract.

  Aliases: gp49B

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review
• transmembrane domain

  protein domain

  A predicted membrane-spanning segment encoded by exon 6 in the gp49B transcript.

  Aliases: TM domain

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewAK (4715169a40) review

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