HY5 regulates anthocyanin biosynthesis by inducing the transcriptional activation of the MYB75/PAP1 transcription factor in Arabidopsis

Several positive transcription factors regulate Arabidopsis anthocyanin biosynthesis. HY5, a component of light‐signaling pathways, and PAP1, an R2R3‐MYB transcription factor, share common regulatory targets on anthocyanin biosynthesis genes. The epistatic interactions between the two transcription factors are currently unknown. To address this problem, we analyzed crosses between hy5 and pap1 mutants (hy5pap1) or pap1D overexpressors (hy5pap1D), performed chromatin immunoprecipitation‐qPCR, and determined the PAP1 promoter region through deletion analysis. The results show that HY5 regulates PAP1 expression via direct binding to G‐ and ACE‐boxes in the promoter region, which suggests bifurcate regulation of anthocyanin biosynthesis by HY5 via transcriptional activation of PAP1.

• Publication year

  2013

• Venue

  FEBS Letters

• Publication date

  2013-05-21

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.febslet.2013.03.037PMID 23583450
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• UV-B photoreceptor-mediated signalling in plants.

  2012cited by this paper
• Sugar-hormone cross-talk in anthocyanin biosynthesis

  2012cited by this paper
• Ethylene Suppression of Sugar-Induced Anthocyanin Pigmentation in Arabidopsis1[C][W][OA]

  2010influential reference
• AtMYBL2, a protein with a single MYB domain, acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis.

  2008cited by this paper
• Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings.

  2008cited by this paper
• Systematic analysis of protein subcellular localization and interaction using high-throughput transient transformation of Arabidopsis seedlings.

  2008cited by this paper
• SOMNUS, a CCCH-Type Zinc Finger Protein in Arabidopsis, Negatively Regulates Light-Dependent Seed Germination Downstream of PIL5[W]

  2008cited by this paper
• Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids.

  2008cited by this paper
• Identification of a cis-regulatory element by transient analysis of co-ordinately regulated genes

  2008cited by this paper
• Expression analysis of anthocyanin regulatory genes in response to different light qualities in Arabidopsis thaliana.

  2008cited by this paper
• PIF3 regulates anthocyanin biosynthesis in an HY5-dependent manner with both factors directly binding anthocyanin biosynthetic gene promoters in Arabidopsis.

  2007influential reference
• Analysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of Development[W]

  2007influential reference
• Sucrose-Specific Induction of Anthocyanin Biosynthesis in Arabidopsis Requires the MYB75/PAP1 Gene 1

  2005influential reference
• Sucrose-Specific Induction of Anthocyanin Biosynthesis in Arabidopsis Requires the MYB75/PAP1 Gene1

  2005influential reference
• Phenotypic characterization of a photomorphogenic mutant.

  2004cited by this paper
• Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis

  2000cited by this paper
• Arabidopsis bZIP Protein HY5 Directly Interacts with Light-Responsive Promoters in Mediating Light Control of Gene Expression

  1998cited by this paper
• The Arabidopsis HY5 gene encodes a bZIP protein that regulates stimulus-induced development of root and hypocotyl.

  1997cited by this paper
• Phytochrome A and Phytochrome B Have Overlapping but Distinct Functions in Arabidopsis Development

  1994cited by this paper
• HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor

  1993cited by this paper
• GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

  1987cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Received Date: 03-Sep-2010 Revised Date: 25-Oct-2010 Accepted Date: 10-Nov-2010 Article type: Full Paper

  year unknowncited by this paper

• Multi-Omics Analysis Provides Insights into Developmental Tepal Coloration in Gloriosa superba ‘Passion Flame’

  2026cites this paper
• Why juvenile reddening occurs in many plants

  2026cites this paper
• Multiomics Analysis Revealed the Role of LsBBX22-LsHY5a/b Module in Promoting UV-B-Mediated Anthocyanin Accumulation in Lettuce.

  2026cites this paper
• CaBBX16 Responds to Light Irradiation and Regulates the Biosynthesis of Anthocyanins in Pepper Leaves.

  2026cites this paper
• Unleashing the Health Potential of Anthocyanins in Crops: An Integrative Perspective From Biosynthesis to Storage

  2026cites this paper
• Beyond HY5: COP1 Posttranslational Control of Anthocyanin Biosynthesis Proteins in Horticultural Crops.

  2026cites this paper
• Natural variation in the promoter of TaHY5 enhances anthocyanin biosynthesis via upregulated transcription of TaF3H and TaANS in purple grain wheat (Triticum aestivum L.).

  2025cites this paper
• Comparative transcriptome analysis of leaf color in 'Shine Muscat' and 'ZhongShan-HongYu' hybrids

  2025cites this paper
• Effects of dark treatment on 'Yinhongli' plum phenolic biosynthesis and antioxidant capacity analysed through transcriptomic and metabolomic approaches

  2025cites this paper
• Characterization of the Lonicera japonica R2R3-MYB transcription factor gene LjaMYB305 that promotes the flavonoid biosynthesis.

  2025cites this paper
• Functional characterization of CiHY5 in salt tolerance of Chrysanthemum indicum and conserved role of HY5 under stress in chrysanthemum.

  2025cites this paper
• SnRK1 acts upstream of PRODUCTION OF ANTHOCYANIN PIGMENT1, contributing to fine-tuning of flavonoid biosynthesis during high-light acclimation.

  2025cites this paper
• CmBBX28-CmMYB9a Module Regulates Petal Anthocyanin Accumulation in Response to Light in Chrysanthemum.

  2025cites this paper
• Integrated metabolomic and transcriptomic analysis of anthocyanin metabolism in wheat pericarp

  2025cites this paper
• Transcriptional survey of the light-induced anthocyanin pathway in non-GM purple tomatoes

  2025cites this paper
• Transcriptomic and targeted metabolomic analysis identifies genes involved in differential anthocyanin accumulation in potato tubers

  2025cites this paper
• Characteristics and Transcriptome Analysis of Anther Abortion in Male Sterile Celery (Apium graveolens L.)

  2025cites this paper
• The HY5-NPR1 module governs light-dependent virulence of a plant bacterial pathogen.

  2025cites this paper
• The MdSINA1-MdWOX8-MdHY5 module links strigolactone and gibberellin signals via interaction with MdSMXL8 and MdRGL3a in the regulation of anthocyanin biosynthesis in apple.

  2025cites this paper
• The ATAF1‐AP2/ACE1‐MYB regulatory cascade negatively orchestrates flower color and scent formation in Freesia hybrida

  2025cites this paper
• AcMYB88 positively regulates anthocyanin biosynthesis in kiwifruit

  2025cites this paper
• Soybean MYB diversification identifies GmMYBA1 as a light-responsive anthocyanin activator

  2025cites this paper
• Molecular mechanism of light-responsive transcription factor ELONGATED HYPOCOTYL 5 regulating anthocyanin synthesis in tea (Camellia sinensis).

  2025cites this paper
• Adaptive responses of plants to light stress: mechanisms of photoprotection and acclimation. A review

  2025cites this paper
• PsHY5 functions as a positive transcription factor of light-induced anthocyanin accumulation in Paeonia suffruticosa cut flowers

  2025cites this paper
• CsHY5 Regulates Light-Induced Anthocyanin Accumulation in Camellia sinensis

  2025cites this paper
• A comprehensive review of transcription factor-mediated regulation of secondary metabolites in plants under environmental stress

  2025cites this paper
• Eggplant transcription factor SmERF118 mediates light signaling regulation of anthocyanin synthesis.

  2025cites this paper
• Transcript profiling of plastid ferrochelatase two mutants reveals that chloroplast singlet oxygen signals lead to global changes in RNA profiles and are mediated by Plant U-Box 4

  2025cites this paper
• Transcriptional regulation of development by SMAX1-LIKE proteins, targets of strigolactone and karrikin/KAI2 ligand signaling.

  2025cites this paper
• HISTONE DEACETYLASE 19 interacts with ELONGATED HYPOCOTYL5 to regulate wheat cuticular wax biosynthesis in response to ultraviolet B radiation.

  2025cites this paper
• Genetic determinants of the flower color transition trait in autotetraploid rose populations

  2025cites this paper
• Advancing Light-Mediated Technology in Plant Growth and Development: The Role of Blue Light

  2025cites this paper
• Diversity and ecological functions of anthocyanins

  2025cites this paper
• A histone deacetylase complex regulates anthocyanin biosynthesis during normal plant growth and development and in response to jasmonate

  2025cites this paper
• The GmMYB1-GmbHLHA-GmCPC-like module regulates light-induced anthocyanin production in soybean sprouts.

  2025cites this paper
• Integrative ATAC-Seq and RNA-Seq Analysis Identifies a WD40 Repeat Protein, ObWPA, as a Significant Regulator of the Purple Coloration in Syringa oblata

  2025cites this paper
• Integrated transcriptomic and metabolomic analyses reveal the mechanisms for red fruit coloration in peach cv. ‘Zhongyoupan 9’ under artificial darkness

  2025cites this paper
• The PavDREB1E–PavD8–PavHY5 module integrates light and gibberellin signals to regulate sweet cherry anthocyanin biosynthesis

  2025cites this paper
• Molecular Control of Flower Colour Change in Angiosperms

  2025cites this paper
• Integrated Metabolomic and Transcriptomic Analyses of the Flavonoid Biosynthetic Pathway in Relation to Color Mutation in Roses

  2025cites this paper
• Two B-Box Proteins, GhBBX21 and GhBBX24, Antagonistically Modulate Anthocyanin Biosynthesis in R1 Cotton

  2025cites this paper
• Effects of Nitrogen or Phosphorus Deficiency and Light Treatment on Anthocyanin Biosynthesis in Tomato Hypocotyls

  2025cites this paper
• Genome-wide characterization of B-box gene family in eggplant and functional identification of SmBBX22 in modulating anthocyanin synthesis

  2025cites this paper
• Apple EBF1/CML15–VQ8 module bridges ethylene and calcium signaling to modulate salt‐induced anthocyanin accumulation

  2025cites this paper
• A BBX-HY5 photomorphogenic module induces anthocyanin synthesis in purple tomato fruits

  2025cites this paper
• Identification of the Cinnamyl Alcohol Dehydrogenase Gene Family in Brassica U-Triangle Species and Its Potential Roles in Response to Abiotic Stress and Regulation of Seed Coat Color in Brassica napus L.

  2025cites this paper
• GLK2, a GOLDEN2‐LIKE Transcription Factor, Directly Regulates Anthocyanin Accumulation by Binding With Promoters of Key Anthocyanin Biosynthetic Genes in Arabidopsis

  2025cites this paper
• Disruption of ABI4 Enhances Anthocyanin Accumulation in Arabidopsis Seedlings Through HY5-Mediated Light Signaling

  2025influential citation
• Molecular Mechanisms Underlying Floral Development Mediated by Blue Light and Other Integrated Signals: Research Findings and Perspectives

  2025cites this paper
• Multiple Factors Regulate Anthocyanin Biosynthesis in Purple-Leaf Tea Varieties in CsMYB75-Dependent and Independent Mechanisms.

  2025cites this paper
• MYB Transcription Factors in Plant Developmental Plasticity

  2025cites this paper
• Postharvest UV-A treatment promotes mango fruit pigmentation and ripening in a dose-dependent manner

  2025cites this paper
• LvWRKY75 enhances the transcription of LvMYB5 and promotes anthocyanin biosynthesis in lily petals during the blooming phase

  2025influential citation
• Anthocyanin Metabolism Associated with Tea Polyphenol-Alleviating Effect on Salinity Toxicity in Wheat Leave

  2025cites this paper
• Under the rainbow: Novel insights on the mechanisms driving the development and evolution of petal pigmentation.

  2025cites this paper
• Integration of transcriptomic and metabolomic analysis reveals light-regulated anthocyanin accumulation in the peel of ‘Yinhongli’ plum

  2025cites this paper
• Low pH attenuates anthocyanin accumulation via the STOP1-MYB75 module in Arabidopsis.

  2025cites this paper
• Putative Light-independent Pathways Regulating Anthocyanin Biosynthesis in Horticultural Plants

  2025cites this paper
• BrGSTF12, an anthocyanin-related glutathione S-transferase gene, is essential for light-induced anthocyanin accumulation in zicaitai (Brassica Rapa Var. purpuraria)

  2025cites this paper
• Anthocyanin pathway in eggplant: genetic regulation and future directions for metabolic engineering

  2025cites this paper
• Loss of DET1 in High Pigment 2 Tomato Prevents High Temperature Repression of Anthocyanin Biosynthesis in Fruit Through HY5 Stabilization.

  2025cites this paper
• Petunia bZIP transcription factor PhbZIP3 is involved in floral volatile benzenoid/phenylpropanoid biosynthesis through regulating the expression of PhPAL2 and PhBSMT

  2024cites this paper
• Development of a fast and efficient root transgenic system for exploring the function of RsMYB90 involved in the anthocyanin biosynthesis of radish

  2024cites this paper
• Transcriptomic Insights into Higher Anthocyanin Accumulation in ‘Summer Black’ Table Grapes in Winter Crop Under Double-Cropping Viticulture System

  2024cites this paper
• Why the adventitious roots of poplar are so colorful: RNAseq and metabolomic analysis reveal anthocyanin accumulation in canker pathogens-induced adventitious roots in poplar

  2024cites this paper
• R2R3-MYB repressor, BrMYB32, regulates anthocyanin biosynthesis in Chinese cabbage.

  2024cites this paper
• Optimizing light conditions for enhanced strawberry growth in tropical lowlands

  2024cites this paper
• bZIP Transcription Factor PavbZIP6 Regulates Anthocyanin Accumulation by Increasing Abscisic Acid in Sweet Cherry

  2024cites this paper
• Transcriptome Analysis Identified PyNAC42 as a Positive Regulator of Anthocyanin Biosynthesis Induced by Nitrogen Deficiency in Pear (Pyrus spp.)

  2024cites this paper
• A negative feedback regulatory module comprising R3-MYB repressor MYBL2 and R2R3-MYB activator PAP1 fine-tunes high light-induced anthocyanin biosynthesis in Arabidopsis.

  2024cites this paper
• ArMYB89 and ArCOP1 interaction modulates anthocyanin biosynthesis in Acer rubrum leaves under low-temperature conditions.

  2024cites this paper
• Transcriptomic and Metabolic Analysis Reveals Genes and Pathways Associated with Flesh Pigmentation in Potato (Solanum tuberosum) Tubers

  2024cites this paper
• A distinct foliar pigmentation pattern formed by activator-repressor gradients upstream of an anthocyanin-activating R2R3-MYB

  2024cites this paper
• The SMXL8-AGL9 module mediates crosstalk between strigolactone and gibberellin to regulate strigolactone-induced anthocyanin biosynthesis in apple.

  2024cites this paper
• CaLAP1 and CaLAP2 orchestrate anthocyanin biosynthesis in the seed coat of Cicer arietinum

  2024cites this paper
• SnRK1 acts upstream of PRODUCTION OF ANTHOCYANIN PIGMENT1, contributing to fine-tuning flavonoid biosynthesis during acclimation

  2024cites this paper
• Advances in functional studies of plant MYC transcription factors

  2024cites this paper
• Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells

  2024cites this paper
• The coordinated interaction or regulation between floral pigments and volatile organic compounds

  2024cites this paper
• Transcript profiling of plastid ferrochelatase two mutants reveals that chloroplast singlet oxygen signals lead to global changes in RNA profiles and are mediated by Plant U-Box 4

  2024cites this paper
• Integrating Physiological Features and Proteomic Analyses Provides New Insights in Blue/Red Light-Treated Moso Bamboo (Phyllostachys edulis).

  2024cites this paper
• Transcription factors BZR1 and PAP1 cooperate to promote anthocyanin biosynthesis in Arabidopsis shoots.

  2024cites this paper
• Cloned genes and genetic regulation of anthocyanin biosynthesis in maize, a comparative review

  2024cites this paper
• Multi-omics analysis reveals anthocyanin synthesis is associated with drought stress tolerance in Chaenomeles speciosa flowers

  2024cites this paper
• The coordinated interaction or regulation between anthocyanin and carotenoid pathways in OT hybrid lilies based on metabolome and time-course transcriptomics analysis

  2024cites this paper
• Postharvest Light Irradiation Induces Anthocyanin Accumulation in Fresh‐Cut Lily Bulb (Lilium davidii var. unicolor) Scales

  2024cites this paper
• Acclimation kinetics of the holoparasitic weed Phelipanche ramosa (Orobanchaceae) during excessive light and heat conditions

  2023cites this paper
• Comprehensive Transcriptome and Metabolome Characterization of Peony ‘Coral Sunset’ Petals Provides Insights into the Mechanism of Pigment Degradation

  2023cites this paper
• Environmental Stimuli and Phytohormones in Anthocyanin Biosynthesis: A Comprehensive Review

  2023influential citation
• The transcription factor SbHY5 mediates light to promote aluminum tolerance by activating SbMATE and SbSTOP1s expression.

  2023cites this paper
• A Novel R2R3–MYB Transcription Factor FaMYB10-like Promotes Light-Induced Anthocyanin Accumulation in Cultivated Strawberry

  2023cites this paper
• Plasticity of Arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditions

  2023cites this paper
• Unveiling the molecular mechanism involving anthocyanins in pineapple peel discoloration during fruit maturation.

  2023cites this paper
• High-quality maple genome reveals duplication-facilitated leaf color diversity.

  2023cites this paper
• Plant bZIP Proteins: Potential use in Agriculture - A Review.

  2023cites this paper
• Eggplant transcription factor SmMYB5 integrates jasmonate and light signaling during anthocyanin biosynthesis.

  2023cites this paper
• The Collaborative Role of the Regulatory (MYB-bHLH-WD40) and Structural Genes Results in Fruit Coloration in Plants Some Do This Under the Influence of External Stimuli

  2023cites this paper
• CmNAC25 targets CmMYB6 to positively regulate anthocyanin biosynthesis during the post-flowering stage in chrysanthemum

  2023cites this paper
• Light regulation of the biosynthesis of phenolics, terpenoids, and alkaloids in plants

  2023cites this paper