Rac1 functions as a reversible tension modulator to stabilize VE-cadherin trans-interaction

Correspondence to Asrar B. Malik: abmalik@uic.edu; or Yulia A. Komarova: ykomarov@uic.edu Abbreviations used in this paper: AJ, adherens junction; CMV, cytomegalovirus; cRO, caged Rockout; DN, dominant negative; FRET, Förster resonance energy transfer; GAP, GTPase-activating protein; HMEC, human dermal microvascular endothelial cell; MLCII, myosin-II light chain; NMR, nuclear magnetic resonance; PA, photoactivatable; PI, photoinsensitive; ROCK, Rho-associated protein kinase; VE-cadherin, vascular endothelial cadherin. Introduction

• Publication year

  2015

• Venue

  Journal of Cell Biology

• Publication date

  2015-04-13

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/jcb.20140910803202015cPMID 25847538PMCID 4395484
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Rac1 functions as a reversible tension modulator to stabilize VE-cadherin trans-interaction

  2015cited by this paper
• Binding to F-actin guides cadherin cluster assembly, stability, and movement

  2013cited by this paper
• A novel pathway spatiotemporally activates Rac1 and redox signaling in response to fluid shear stress

  2013influential reference
• Fluid shear stress on endothelial cells modulates mechanical tension across VE-cadherin and PECAM-1.

  2013cited by this paper
• VE-cadherin and endothelial adherens junctions: active guardians of vascular integrity.

  2013cited by this paper
• Interaction of p190RhoGAP with C-terminal Domain of p120-catenin Modulates Endothelial Cytoskeleton and Permeability*

  2013cited by this paper
• RhoA is down-regulated at cell–cell contacts via p190RhoGAP-B in response to tensional homeostasis

  2013cited by this paper
• A Critical Role for Phosphatidylinositol (3,4,5)-Trisphosphate–Dependent Rac Exchanger 1 in Endothelial Junction Disruption and Vascular Hyperpermeability

  2012cited by this paper
• A photoactivatable small-molecule inhibitor for light-controlled spatiotemporal regulation of Rho kinase in live embryos

  2012cited by this paper
• A model of localised Rac1 activation in endothelial cells due to fluid flow.

  2011cited by this paper
• Ajuba is required for Rac activation and maintenance of E-cadherin adhesion

  2011cited by this paper
• Cadherin exits the junction by switching its adhesive bond

  2011cited by this paper
• The extracellular architecture of adherens junctions revealed by crystal structures of type I cadherins.

  2011cited by this paper
• Caveolin-1–eNOS signaling promotes p190RhoGAP-A nitration and endothelial permeability

  2011cited by this paper
• Structure and binding mechanism of vascular endothelial cadherin: a divergent classical cadherin.

  2011influential reference
• Role of GTPases in control of microvascular permeability.

  2010cited by this paper
• The PI 3 K p 110 isoform regulates endothelial adherens junctions via Pyk 2 and Rac 1

  2010cited by this paper
• Regulation of endothelial permeability via paracellular and transcellular transport pathways.

  2010cited by this paper
• Spontaneous assembly and active disassembly balance adherens junction homeostasis

  2010cited by this paper
• Endocytosis is required for E-cadherin redistribution at mature adherens junctions

  2009cited by this paper
• A genetically-encoded photoactivatable Rac controls the motility of living cells

  2009influential reference
• The Regulation of Vascular Endothelial Growth Factor-induced Microvascular Permeability Requires Rac and Reactive Oxygen Species*

  2009cited by this paper
• Resolving cadherin interactions and binding cooperativity at the single-molecule level

  2009cited by this paper
• Linking molecular affinity and cellular specificity in cadherin-mediated adhesion

  2009influential reference
• Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell–cell adhesion

  2007cited by this paper
• Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2

  2007cited by this paper
• p120-catenin and p190RhoGAP regulate cell-cell adhesion by coordinating antagonism between Rac and Rho.

  2006cited by this paper
• Spatiotemporal dynamics of RhoA activity in migrating cells

  2006influential reference
• A New Photocaging Group for Aromatic N‐Heterocycles.

  2006cited by this paper
• Sphingosine 1-Phosphate-induced Mobilization of Intracellular Ca2+ Mediates Rac Activation and Adherens Junction Assembly in Endothelial Cells*

  2005cited by this paper
• The mechanism of cell adhesion by classical cadherins: the role of domain 1

  2005cited by this paper
• Proteolytic E‐cadherin activation followed by solution NMR and X‐ray crystallography

  2004influential reference
• Cadherin function probed by laser tweezer and single molecule fluorescence in vascular endothelial cells

  2003cited by this paper
• ROCKs: multifunctional kinases in cell behaviour

  2003cited by this paper
• Small GTP-Binding Protein Rac Is an Essential Mediator of Vascular Endothelial Growth Factor-Induced Endothelial Fenestrations and Vascular Permeability

  2003cited by this paper
• Spatio-temporal regulation of Rac1 localization and lamellipodia dynamics during epithelial cell-cell adhesion.

  2002cited by this paper
• E-cadherin Homophilic Ligation Directly Signals through Rac and Phosphatidylinositol 3-Kinase to Regulate Adhesive Contacts*

  2002cited by this paper
• Reactive oxygen species mediate Rac-induced loss of cell-cell adhesion in primary human endothelial cells.

  2002cited by this paper
• VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam.

  2002cited by this paper
• Cadherin Engagement Regulates Rho family GTPases*

  2001cited by this paper
• Real-Time Imaging of Vascular Endothelial-Cadherin During Leukocyte Transmigration Across Endothelium1

  2001cited by this paper
• Cadherin interaction probed by atomic force microscopy.

  2000influential reference
• Vascular endothelial cell adherens junction assembly and morphogenesis induced by sphingosine-1-phosphate.

  1999influential reference
• HMEC-1: establishment of an immortalized human microvascular endothelial cell line.

  1992influential reference

• Bulk exocytosis of large intracellular apical precursor organelles establishes apical domain identity during de novo lumen formation

  2025cites this paper
• Microenvironmental acidification by pneumococcal sugar consumption fosters barrier disruption and immune suppression in the human alveolus

  2024cites this paper
• Tension sensing by FAK governs nuclear mechanotransduction, endothelial transcriptome and fate

  2023cites this paper
• Cadmium exposure enhances VE-cadherin expression in endothelial cells via suppression of ROCK signaling.

  2022cites this paper
• DOCK4 Regulation of Rho GTPases Mediates Pulmonary Vascular Barrier Function

  2022cites this paper
• SHOCK INDUCES ENDOTHELIAL PERMEABILITY AFTER TRAUMA THROUGH INCREASED ACTIVATION OF RHOA GTPASE

  2022influential citation
• Small GTPases orchestrate cell-cell communication during collective cell movement

  2020cites this paper
• Post-translational modifications of S1PR1 and endothelial barrier regulation.

  2020cites this paper
• The vertebrate epithelial apical junctional complex: Dynamic interplay between Rho GTPase activity and cell polarization processes.

  2020cites this paper
• The Importance of Mechanical Forces for in vitro Endothelial Cell Biology

  2020cites this paper
• Building Blood Vessels—One Rho GTPase at a Time

  2019cites this paper
• VE-PTP stabilizes VE-cadherin junctions and the endothelial barrier via a phosphatase-independent mechanism

  2019cites this paper
• Mind the gap: mechanisms regulating the endothelial barrier

  2018cites this paper
• The CCM1–CCM2 complex controls complementary functions of ROCK1 and ROCK2 that are required for endothelial integrity

  2018cites this paper
• SHP2 protects endothelial cell barrier through suppressing VE‐cadherin internalization regulated by MET‐ARF1

  2018cites this paper
• Interplay of GTPases and Cytoskeleton in Cellular Barrier Defects during Gut Inflammation

  2017influential citation
• Protein Interactions at Endothelial Junctions and Signaling Mechanisms Regulating Endothelial Permeability.

  2017influential citation
• Three-dimensional biomimetic vascular model reveals a RhoA, Rac1, and N-cadherin balance in mural cell–endothelial cell-regulated barrier function

  2017cites this paper
• WAVE regulates Cadherin junction assembly and turnover during epithelial polarization.

  2017cites this paper
• Endothelial cell-cell adhesion and signaling

  2017cites this paper
• SOX17 Regulates Conversion of Human Fibroblasts Into Endothelial Cells and Erythroblasts by Dedifferentiation Into CD34+ Progenitor Cells

  2017cites this paper
• Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors

  2017cites this paper
• Force-driven growth of intercellular junctions.

  2017cites this paper
• The Interplay between Cofilin and Phospho-Cofilin: Its Role in Maintaining Blood Brain Barrier Integrity.

  2017cites this paper
• S1P-Mediated Endothelial Barrier Enhancement: Role of Rho Family GTPases and Local Lamellipodia

  2017cites this paper
• Nanoparticle Density: A Critical Biophysical Regulator of Endothelial Permeability.

  2017cites this paper
• RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking the cell border.

  2016cites this paper
• Etiology of intracerebral hemorrhage (ICH): novel insights from Zebrafish embryos.

  2016cites this paper
• Transient receptor potential channel 1 maintains adherens junction plasticity by suppressing sphingosine kinase 1 expression to induce endothelial hyperpermeability

  2016cites this paper
• The pulmonary endothelium in acute respiratory distress syndrome: insights and therapeutic opportunities

  2016cites this paper
• Quantitative dynamics of VE-cadherin at endothelial cell junctions at a glance: basic requirements and current concepts

  2016cites this paper
• Using magnets and magnetic beads to dissect signaling pathways activated by mechanical tension applied to cells.

  2016cites this paper
• Forces and mechanotransduction in 3D vascular biology.

  2016cites this paper
• RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border

  2016cites this paper
• Temporal-specific roles of Rac1 during vascular development and retinal angiogenesis.

  2016cites this paper
• THSD1 preserves vascular integrity and protects against intraplaque haemorrhaging in ApoE-/- mice.

  2016cites this paper
• The Andes Virus Nucleocapsid Protein Directs Basal Endothelial Cell Permeability by Activating RhoA

  2016cites this paper
• Small Rho GTPase-mediated actin dynamics at endothelial adherens junctions

  2016cites this paper
• Cell–cell junctional mechanotransduction in endothelial remodeling

  2016cites this paper
• MRCK-1 Drives Apical Constriction in C. elegans by Linking Developmental Patterning to Force Generation.

  2016cites this paper
• Simvastatin Ameliorates Matrix Stiffness-Mediated Endothelial Monolayer Disruption

  2016influential citation
• Endothelial basement membrane laminin 511 is essential for shear stress response

  2016cites this paper
• ROCK2 primes the endothelium for vascular hyperpermeability responses by raising baseline junctional tension.

  2015cites this paper
• Microtubule-Associated Protein EB3 Regulates IP3 Receptor Clustering and Ca2+ Signaling in Endothelial Cells

  2015cites this paper
• Arterial endothelial methylome: differential DNA methylation in athero-susceptible disturbed flow regions in vivo

  2015cites this paper
• Regulation of Endothelial Adherens Junctions by Tyrosine Phosphorylation

  2015cites this paper
• Allosteric Regulation of E-Cadherin Adhesion*

  2015cites this paper