Root defense analysis against Fusarium oxysporum reveals new regulators to confer resistance

Fusarium oxysporum is a root-infecting fungal pathogen that causes wilt disease on a broad range of plant species, including Arabidopsis thaliana. Investigation of the defense response against this pathogen had primarily been conducted using leaf tissue and little was known about the root defense response. In this study, we profiled the expression of root genes after infection with F. oxysporum by microarray analysis. In contrast to the leaf response, root tissue did not show a strong induction of defense-associated gene expression and instead showed a greater proportion of repressed genes. Screening insertion mutants from differentially expressed genes in the microarray uncovered a role for the transcription factor ETHYLENE RESPONSE FACTOR72 (ERF72) in susceptibility to F. oxysporum. Due to the role of ERF72 in suppressing programmed cell death and detoxifying reactive oxygen species (ROS), we examined the pub22/pub23/pub24 U-box type E3 ubiquitin ligase triple mutant which is known to possess enhanced ROS production in response to pathogen challenge. We found that the pub22/23/24 mutant is more resistant to F. oxysporum infection, suggesting that a heightened innate immune response provides protection against F. oxysporum. We conclude that root-mediated defenses against soil-borne pathogens can be provided at multiple levels.

• Publication year

  2014

• Venue

  Scientific Reports

• Publication date

  2014-07-07

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/srep05584PMID 24998294PMCID 4083284
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2013influential reference
• Arabidopsis thaliana RESISTANCE TO FUSARIUM OXYSPORUM 2 Implicates Tyrosine-Sulfated Peptide Signaling in Susceptibility and Resistance to Root Infection

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  2012cited by this paper
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  2012cited by this paper
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  2012cited by this paper
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  2011cited by this paper
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  2010cited by this paper
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  2010influential reference
• Electronic correlations at the α-γ structural phase transition in paramagnetic iron.

  2010cited by this paper
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  2008cited by this paper
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  2008cited by this paper
• Arabidopsis systemic immunity uses conserved defense signaling pathways and is mediated by jasmonates

  2007cited by this paper
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  1999cited by this paper
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  1991cited by this paper
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  1991cited by this paper

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