Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems

No abstract is available for this paper.

• Publication year

  2010

• Venue

  Biotechnology Letters

• Publication date

  2010-05-13

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1007/s10529-010-0293-xPMID 20464452
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Stem cell- and scaffold-based tissue engineering approaches to osteochondral regenerative medicine.

  2009influential reference
• Derivation, characterization and gene modification of cynomolgus monkey mesenchymal stem cells.

  2009cited by this paper
• The use of mesenchymal stem cells for chondrogenesis.

  2008cited by this paper
• Hypoxia promotes chondrogenesis in rat mesenchymal stem cells: A role for AKT and hypoxia‐inducible factor (HIF)‐1α

  2008cited by this paper
• Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem cells.

  2008cited by this paper
• High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.

  2007cited by this paper
• Three-dimensional cartilage tissue engineering using adult stem cells from osteoarthritis patients.

  2007cited by this paper
• Chondrogenic Potential of Human Adult Mesenchymal Stem Cells Is Independent of Age or Osteoarthritis Etiology

  2007cited by this paper
• Chondrogenic Differentiation of Human Bone Marrow Stem Cells in Transwell Cultures: Generation of Scaffold‐Free Cartilage

  2007cited by this paper
• Reduced oxygen tension attenuates differentiation capacity of human mesenchymal stem cells and prolongs their lifespan

  2007cited by this paper
• Engineered mesenchymal stem cells for cartilage repair.

  2006cited by this paper
• Effect of reduced oxygen tension on chondrogenesis and osteogenesis in adipose-derived mesenchymal cells.

  2006cited by this paper
• High-throughput aggregate culture system to assess the chondrogenic potential of mesenchymal stem cells.

  2005cited by this paper
• Tissue engineering strategies for cartilage generation--micromass and three dimensional cultures using human chondrocytes and a continuous cell line.

  2005cited by this paper
• Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo.

  2002cited by this paper
• High density micromass cultures of embryonic limb bud mesenchymal cells: An in vitro model of endochondral skeletal development

  1999cited by this paper
• Multilineage potential of adult human mesenchymal stem cells.

  1999influential reference
• In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells.

  1998cited by this paper

• Synergistic Overexpression of Sox9, TGFβ1, and Col II Induces Functional Chondrogenesis in hUC-MSCs Using a 3D Culture Approach

  2026cites this paper
• Enhanced collagen deposition in chondrogenic inflammation with Ibuprofen-specifically loaded chitosan/poly-gamma-glutamic acid nanoparticles.

  2025cites this paper
• Co-Culture Approaches in Cartilage and Bone Tissue Regeneration

  2025cites this paper
• The Impact of Mechanical Bioreactors on Human Mesenchymal Stromal Cells Utilized for Articular Cartilage Repair.

  2025cites this paper
• Donor Variability and 3D Culture Models Influence Human Mesenchymal Stem Cell Differentiation

  2025cites this paper
• Three-dimensional cell spheroid technology: Recent advances and emerging strategies in cartilage regeneration.

  2025cites this paper
• Exploring BMP9 and integrin alpha 10 as novel chondrogenic markers in human bone marrow-derived mesenchymal stem cells, chondroprogenitors, and chondrocytes: an immunohistochemistry study.

  2025cites this paper
• Scaffold-Free Osteochondral Engineering Using Embryonic-Derived Mesenchymal Stem Cell Spheroids.

  2025cites this paper
• Donor-dependent regulation of type II and X collagen deposition by early modulation of miR-335-5p and miR-1246 during chondrogenic commitment

  2025cites this paper
• Effect of serum on chondrogenic differentiation and proliferation of 2D cultured rat bone marrow mesenchymal stem cells

  2025cites this paper
• Encapsulation of Transforming Growth Factor-β3 in Poly(hydroxybutyrate-co-hydroxyvalerate) Nanoparticles for Enhanced Cartilage Tissue Engineering

  2025cites this paper
• Influence of Cell Seeding Density and Material Stiffness on Chondrogenesis of Human Stem Cells Within Soft Hydrogels, Without the Use of Exogenous Growth Factors

  2025cites this paper
• Dynamic transcriptional and epigenetic changes define postnatal tendon growth

  2025cites this paper
• Monitoring the kinetic evolution of mesenchymal stem cell differentiation using Raman microspectroscopy.

  2025cites this paper
• Biomimetic Glycosaminoglycan-Enriched Electrospun Polymeric Scaffolds for Enhanced Early Tissue Regeneration

  2025cites this paper
• Matrigel-encapsulated articular cartilage derived fibronectin adhesion assay derived chondroprogenitors for enhanced chondrogenic differentiation: An in vitro evaluation.

  2024cites this paper
• Extremely low frequency–electromagnetic fields promote chondrogenic differentiation of adipose-derived mesenchymal stem cells through a conventional genetic program

  2024influential citation
• Cellular Interactions and Biological Effects of Silk Fibroin: Implications for Tissue Engineering and Regenerative Medicine.

  2024cites this paper
• Mesenchymal stem cells derived from human adipose tissue exhibit significantly higher chondrogenic differentiation potential compared to those from rats.

  2024cites this paper
• Tumoroids, a valid preclinical screening platform for monitoring cancer angiogenesis

  2024cites this paper
• Small spheroids for head and neck cartilage tissue engineering

  2024cites this paper
• Metabolic modulation to improve MSC expansion and therapeutic potential for articular cartilage repair

  2024cites this paper
• Unlocking Potential: Low Bovine Serum Albumin Enhances the Chondrogenicity of Human Adipose-Derived Stromal Cells in Pellet Cultures

  2024cites this paper
• In Vitro Characterization of Reversine-Treated Gingival Fibroblasts and Their Safety Evaluation after In Vivo Transplantation

  2024cites this paper
• Recent advancements in cartilage tissue engineering innovation and translation

  2024cites this paper
• Microphysiological System‐Generated Physiological Shear Forces Reduce TNF‐α‐Mediated Cartilage Damage in a 3D Model of Arthritis

  2024cites this paper
• A Review on Patient-Derived 3D Micro Cancer Approach for Drug Screen in Personalized Cancer Medicine.

  2024cites this paper
• Evolutionary Grid Optimization and Deep Learning for Improved In Vitro Cellular Spheroid Localization

  2024cites this paper
• Multifunctional Mesoporous Polydopamine Near-Infrared Photothermal Controlled Release of Kartogenin for Cartilage Repair

  2023cites this paper
• Spheroid Culture System, a Promising Method for Chondrogenic Differentiation of Dental Mesenchymal Stem Cells

  2023cites this paper
• Regulation of cell fate by cell imprinting approach in vitro

  2023cites this paper
• Unfolding the Mystery Behind the Onset of Chondrocyte Hypertrophy during Chondrogenesis: Toward Designing Advanced Permanent Cartilage‐mimetic Biomaterials

  2023cites this paper
• Three-dimensional, in-vitro approaches for modelling soft-tissue joint diseases.

  2023cites this paper
• Human fetal cartilage-derived chondrocytes and chondroprogenitors display a greater commitment to chondrogenesis than adult cartilage resident cells

  2023cites this paper
• Sericin nano-gel agglomerates mimicking the pericellular matrix induce the condensation of mesenchymal stem cells and trigger cartilage micro-tissue formation without exogenous stimulation of growth factors in vitro.

  2023cites this paper
• Converse modulation of Wnt/β-catenin signaling during expansion and differentiation phases of Infrapatellar fat pad-derived MSCs for improved engineering of hyaline cartilage.

  2023cites this paper
• In Vitro Quantification of Cellular Spheroids in Patterned Petri Dishes

  2023cites this paper
• Physical and Biological Characterizations of Injectable Thermosensitive Poly(N-Isopropylacrylamide)-Collagen I Hydrogels Intended for Meniscus Repair

  2023cites this paper
• Applications and Utility of Three-Dimensional In Vitro Cell Culture for Therapeutics

  2023cites this paper
• Pre-culture of human mesenchymal stromal cells in spheroids facilitates chondrogenesis at a low total cell count upon embedding in biomaterials to generate cartilage microtissues.

  2022cites this paper
• Spatial patterning of phenotypically distinct microtissues to engineer osteochondral grafts for biological joint resurfacing.

  2022cites this paper
• Engineering High-Quality Cartilage Microtissues Using Hydrocortisone Functionalized Microwells

  2022cites this paper
• Articular Cartilage Regeneration in Veterinary Medicine.

  2022cites this paper
• Mesenchymal stromal/stem cells spheroid culture effect on the therapeutic efficacy of these cells and their exosomes: A new strategy to overcome cell therapy limitations.

  2022cites this paper
• Chondrogenesis of Adipose-Derived Stem Cells Using an Arrayed Spheroid Format.

  2022cites this paper
• In vitro Exposure to Inflammatory Mediators Affects the Differentiation of Mesenchymal Progenitors

  2022cites this paper
• The Role of Zinc in Bone Mesenchymal Stem Cell Differentiation

  2022cites this paper
• Periodontal tissue regeneration by transplantation of autologous adipose tissue-derived multi-lineage progenitor cells

  2022cites this paper
• Sulfated GAG mimetic peptide nanofibers enhance chondrogenic differentiation of mesenchymal stem cells in 3D in vitro models

  2022cites this paper
• Comparison of biological characteristics of human adipose- and umbilical cord- derived mesenchymal stem cells and their effects on delaying the progression of osteoarthritis in a rat model.

  2022cites this paper
• Mesenchymal stromal cell-based therapy for cartilage regeneration in knee osteoarthritis

  2022cites this paper
• Soft Hydrogel Environments that Facilitate Cell Spreading and Aggregation Preferentially Support Chondrogenesis of Adult Stem Cells.

  2022cites this paper
• Chondrogenic Potential of Human Dental Pulp Stem Cells Cultured as Microtissues

  2021cites this paper
• M1-like macrophage contributes to chondrogenesis in vitro

  2021cites this paper
• Methods of Modification of Mesenchymal Stem Cells and Conditions of Their Culturing for Hyaline Cartilage Tissue Engineering

  2021cites this paper
• Biomaterials-assisted spheroid engineering for regenerative therapy

  2021cites this paper
• An improved method for processing chondroprogenitor pellets following chondrogenic differentiation for histology and immunohistochemical staining using agarose

  2021cites this paper
• Melatonin contributes to the hypertrophic differentiation of mesenchymal stem cell-derived chondrocytes via activation of the Wnt/β-catenin signaling pathway

  2021cites this paper
• AN ANCIENT DOSAGE FORM “GUTIKA”: A REVIEW

  2021cites this paper
• Tuning the Phenotype of Cartilage Tissue Mimics by Varying Spheroid Maturation and Methacrylamide-Modified Gelatin Hydrogel Characteristics.

  2021cites this paper
• Trends in Articular Cartilage Tissue Engineering: 3D Mesenchymal Stem Cell Sheets as Candidates for Engineered Hyaline-Like Cartilage

  2021cites this paper
• Synovial fluid niche promoted differentiation of dental follicle mesenchymal stem cells toward chondrogenesis in rheumatoid arthritis

  2021cites this paper
• Perichondrium-inspired permeable nanofibrous tube well promoting differentiation of hiPSC-derived pellet toward hyaline-like cartilage pellet

  2021cites this paper
• Autonomous spheroid formation by culture plate compartmentation

  2021cites this paper
• Organoid and Spheroid Tumor Models: Techniques and Applications

  2021cites this paper
• Ex Vivo Systems to Study Chondrogenic Differentiation and Cartilage Integration

  2021cites this paper
• Current Models for Development of Disease-Modifying Osteoarthritis Drugs

  2021cites this paper
• Dental Pulp-Derived Mesenchymal Stem Cells for Modeling Genetic Disorders

  2021cites this paper
• Differentiation of adipose-derived stem cells to chondrocytes using electrospraying

  2021cites this paper
• Biofabrication and bioprinting using cellular aggregates, microtissues and organoids for the engineering of musculoskeletal tissues.

  2021cites this paper
• The effect of synthesized cartilage tissue from human adipose-derived mesenchymal stem cells in orthopedic spine surgery in patients with osteoarthritis.

  2021cites this paper
• A Roadmap of In Vitro Models in Osteoarthritis: A Focus on Their Biological Relevance in Regenerative Medicine

  2021cites this paper
• Human mesenchymal stem cell therapy for cartilage repair: Review on isolation, expansion, and constructs.

  2020cites this paper
• Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow−/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia

  2020cites this paper
• Osteochondral repair combining therapeutics implant with Mesenchymal stem cells spheroids.

  2020cites this paper
• Up-Regulation of Superoxide Dismutase 2 in 3D Spheroid Formation Promotes Therapeutic Potency of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells

  2020cites this paper
• Histone ChIP‐Seq identifies differential enhancer usage during chondrogenesis as critical for defining cell‐type specificity

  2020cites this paper
• Modular Orthopaedic Tissue Engineering With Implantable Microcarriers and Canine Adipose-Derived Mesenchymal Stromal Cells

  2020cites this paper
• In vivo regeneration of rat laryngeal cartilage with mesenchymal stem cells derived from human induced pluripotent stem cells via neural crest cells.

  2020cites this paper
• derived from mesenchymal stromal cells and induced pluripotent cells of patients with familial dissecans exhibit an endoplasmic reticulum stress response and defective matrix assembly STEM

  2020cites this paper
• The effect of PLLA/PVA nanofibrous scaffold on the chondrogenesis of human induced pluripotent stem cells

  2020influential citation
• Gene Profiles in the Early Stage of Neuronal Differentiation of Mouse Bone Marrow Stromal Cells Induced by Basic Fibroblast Growth Factor

  2020cites this paper
• Which Culture System Is better for Chondrogenesis of Adipose-Derived Stem Cells ; Pellet or Micromass?

  2020cites this paper
• Combined Biomaterials: Amniotic Membrane and Adipose Tissue to Restore Injured Bone as Promoter of Calcification in Bone Regeneration: Preclinical Model

  2020cites this paper
• From biofilm disinfection to bacterial stem cell modulation and dentin matrix-driven stem cell mineralization

  2020cites this paper
• Scaffold-Free Cartilage Construct from Infrapatellar Fat Pad Stem Cells for Cartilage Restoration

  2020cites this paper
• Osteochondral Injury, Management and Tissue Engineering Approaches

  2020cites this paper
• Engineered Microsystems for Spheroid and Organoid Studies

  2020cites this paper
• Cell Immobilization Strategies for Tissue Engineering: Recent Trends and Future Perspectives

  2020cites this paper
• Mesenchymal Stem Cells as a Promising Cell Source for Integration in Novel In Vitro Models

  2020cites this paper
• Calibrated Frequency-Difference Electrical Impedance Tomography for 3D Tissue Culture Monitoring

  2019cites this paper
• Quantification of chondrogenic differentiation in monolayer cultures of mesenchymal stromal cells.

  2019cites this paper
• In Vitro Analysis of the Differentiation Capacity of Postmortally Isolated Human Chondrocytes Influenced by Different Growth Factors and Oxygen Levels

  2019cites this paper
• In vivo articular cartilage regeneration through infrapatellar adipose tissue derived stem cell in nanofiber polycaprolactone scaffold.

  2019cites this paper
• Amniotic fluid: Source of valuable mesenchymal stem cells and alternatively used as cryopreserved solution.

  2019cites this paper
• Gelatin microspheres releasing transforming growth factor drive in vitro chondrogenesis of human periosteum derived cells in micromass culture.

  2019cites this paper
• Adipose Tissue-Derived Stem Cells Have the Ability to Differentiate into Alveolar Epithelial Cells and Ameliorate Lung Injury Caused by Elastase-Induced Emphysema in Mice

  2019cites this paper
• Serum-Free Culture of Human Mesenchymal Stem Cell Aggregates in Suspension Bioreactors for Tissue Engineering Applications

  2019cites this paper
• In Vitro and In Vivo Comparison of Different Types of Rabbit Mesenchymal Stem Cells for Cartilage Repair

  2019cites this paper
• Molecular cargo delivery using multicellular magnetic microswimmers

  2019cites this paper