Objective Changes to the microbial community in the human gut have been proposed to promote metabolic disturbances that also occur after short periods of sleep loss (including insulin resistance). However, whether sleep loss affects the gut microbiota remains unknown. Methods In a randomized within-subject crossover study utilizing a standardized in-lab protocol (with fixed meal times and exercise schedules), we studied nine normal-weight men at two occasions: after two nights of partial sleep deprivation (PSD; sleep opportunity 02:45–07:00 h), and after two nights of normal sleep (NS; sleep opportunity 22:30–07:00 h). Fecal samples were collected within 24 h before, and after two in-lab nights, of either NS or PSD. In addition, participants underwent an oral glucose tolerance test following each sleep intervention. Results Microbiota composition analysis (V4 16S rRNA gene sequencing) revealed that after two days of PSD vs. after two days of NS, individuals exhibited an increased Firmicutes:Bacteroidetes ratio, higher abundances of the families Coriobacteriaceae and Erysipelotrichaceae, and lower abundance of Tenericutes (all P < 0.05) – previously all associated with metabolic perturbations in animal or human models. However, no PSD vs. NS effect on beta diversity or on fecal short-chain fatty acid concentrations was found. Fasting and postprandial insulin sensitivity decreased after PSD vs. NS (all P < 0.05). Discussion Our findings demonstrate that short-term sleep loss induces subtle effects on human microbiota. To what extent the observed changes to the microbial community contribute to metabolic consequences of sleep loss warrants further investigations in larger and more prolonged sleep studies, to also assess how sleep loss impacts the microbiota in individuals who already are metabolically compromised.
Gut microbiota and glucometabolic alterations in response to recurrent partial sleep deprivation in normal-weight young individuals
C. Benedict,H. Vogel,W. Jonas,Anni Woting,M. Blaut,A. Schürmann,J. Cedernaes
Published 2016 in Molecular Metabolism
ABSTRACT
PUBLICATION RECORD
- Publication year
2016
- Venue
Molecular Metabolism
- Publication date
2016-10-24
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- beta diversity
A measure of between-sample differences in microbial community composition.
- coriobacteriaceae
A bacterial family measured in the fecal microbiota sequencing profile.
- erysipelotrichaceae
A bacterial family measured in the fecal microbiota sequencing profile.
- fecal short-chain fatty acids
Short-chain fatty acid metabolites measured in stool samples.
Aliases: SCFAs
- firmicutes:bacteroidetes ratio
A compositional metric comparing the relative abundance of the Firmicutes and Bacteroidetes phyla in gut samples.
Aliases: Firmicutes:Bacteroidetes, F:B ratio
- insulin sensitivity
The responsiveness to insulin assessed as fasting and postprandial metabolic handling after each sleep condition.
- normal sleep
A two-night control condition with sleep opportunity from 22:30 to 07:00 h, abbreviated NS in the abstract.
Aliases: NS
- partial sleep deprivation
A two-night sleep restriction condition with sleep opportunity from 02:45 to 07:00 h, abbreviated PSD in the abstract.
Aliases: PSD
- tenericutes
A bacterial phylum detected as part of the fecal microbiota sequencing profile.
REFERENCES
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