The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3'.

No abstract is available for this paper.

• Publication year

  1997

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  1997-06-24

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1073/PNAS.94.13.6706PMID 9192629PMCID PMC21222
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Function of E. coli RNA polymerase sigma factor sigma 70 in promoter-proximal pausing.

  1996cited by this paper
• Base-specific recognition of the nontemplate strand of promoter DNA by E. coli RNA polymerase.

  1996cited by this paper
• Role of the Escherichia coli Recombination Hotspot, χ, in RecABCD-dependent Homologous Pairing (*)

  1995cited by this paper
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  1994cited by this paper
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  1994cited by this paper
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  1994cited by this paper
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  1994cited by this paper
• Mutations in sigma factor that affect the temperature dependence of transcription from a promoter, but not from a mismatch bubble in double-stranded DNA.

  1994cited by this paper
• Biochemistry of homologous recombination in Escherichia coli.

  1994cited by this paper
• In vitro reconstitution of homologous recombination reactions

  1994cited by this paper
• Strand-specific binding to duplex DNA ends by the subunits of the Escherichia coli RecBCD enzyme.

  1993cited by this paper
• The recombination hotspot χ is a regulatory sequence that acts by attenuating the nuclease activity of the E. coli RecBCD enzyme

  1993cited by this paper
• Chi sequence protects against RecBCD degradation of DNA in vivo.

  1992cited by this paper
• Functional transcription elongation complexes from synthetic RNA-DNA bubble duplexes.

  1992cited by this paper
• Processivity of the DNA helicase activity of Escherichia coli recBCD enzyme.

  1992cited by this paper
• Effect of nucleotide cofactor structure on recA protein-promoted DNA pairing. 2. DNA renaturation reaction.

  1992cited by this paper
• Directed mutagenesis : a practical approach

  1991cited by this paper
• Homologous pairing in vitro stimulated by the recombination hotspot, Chi.

  1991cited by this paper
• RecBCD-dependent joint molecule formation promoted by the Escherichia coli RecA and SSB proteins.

  1991cited by this paper
• Formation of heteroduplex DNA promoted by the combined activities of Escherichia coli recA and recBCD proteins.

  1989cited by this paper
• Characterization of the helicase activity of the Escherichia coli RecBCD enzyme using a novel helicase assay.

  1989cited by this paper
• Nucleic Acids and Molecular Biology

  1988cited by this paper
• Substrate specificity of the DNA unwinding activity of the RecBC enzyme of Escherichia coli.

  1985cited by this paper
• RecBC enzyme nicking at Chi sites during DNA unwinding: location and orientation-dependence of the cutting.

  1985cited by this paper
• Chi-dependent DNA strand cleavage by RecBC enzyme.

  1985cited by this paper
• Chi hotspots of generalized recombination.

  1983cited by this paper
• Structure of chi hotspots of generalized recombination.

  1981cited by this paper
• Nucleotide sequence of the chi recombinational hot spot chi +D in bacteriophage lambda

  1981cited by this paper
• A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

  1979cited by this paper
• Recombination pathway specificity of Chi.

  1977cited by this paper
• Selective inhibition of the dnase activity of the recBC enzyme by the DNA binding protein from Escherichia coli.

  1976cited by this paper
• Rec-mediated recombinational hot spot activity in bacteriophage lambda. III. Chi mutations are site-mutations stimulating rec-mediated recombination.

  1975cited by this paper
• Rec-mediated recombinational hot spot activity in bacteriophage lambda. I. Hot spot activity associated with spi-deletions and bio substitutions.

  1974cited by this paper
• Rec-mediated recombinational hot spot activity in bacteriophage λ

  1974cited by this paper
• Physical and Topological Properties of Circular DNA

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• Biocontrol of Phage Resistance in Pseudomonas Infections: Insights into Directed Breaking of Spontaneous Evolutionary Selection in Phage Therapy

  2025cites this paper
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  2024cites this paper
• Trans-complementation by the RecB nuclease domain of RecBCD enzyme reveals new insight into RecA loading upon χ recognition

  2024cites this paper
• E. coli RecB Nuclease Domain Regulates RecBCD Helicase Activity but not Single Stranded DNA Translocase Activity.

  2023cites this paper
• Trans-complementation by the RecB nuclease domain of RecBCD enzyme reveals new insight into RecA loading upon χ recognition

  2023cites this paper
• E. coli RecBCD Nuclease Domain Regulates Helicase Activity but not Single Stranded DNA Translocase Activity

  2023cites this paper
• RecBCD enzyme: mechanistic insights from mutants of a complex helicase-nuclease

  2023cites this paper
• Identification of small-molecule inhibitors of the DNA repair proteins RuvAB from Pseudomonas aeruginosa.

  2022cites this paper
• Long DNA constructs to study helicases and nucleic acid translocases using optical tweezers.

  2022cites this paper
• Structures of RecBCD in complex with phage-encoded inhibitor proteins reveal distinctive strategies for evasion of a bacterial immunity hub

  2022cites this paper
• SURVEY AND SUMMARY Single-molecule insight into stalled replication fork rescue in Escherichiacoli

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• Single-molecule insight into stalled replication fork rescue in Escherichia coli

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• Heterogeneity in E. coli RecBCD Helicase-DNA binding and base pair melting.

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• DNA Repair in Staphylococcus aureus

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• Akaby—Cell-free protein expression system for linear templates

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• A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair

  2020cites this paper
• Distribution of RecBCD and AddAB recombination-associated genes among bacteria in 33 phyla.

  2020cites this paper
• A conformational switch in response to Chi binding converts RecBCD from phage destruction to DNA repair

  2020cites this paper
• Identification of a potent small-molecule inhibitor of bacterial DNA repair that potentiates quinolone antibiotic activity in methicillin-resistant Staphylococcus aureus☆

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• A conformational switch in response to Chi converts RecBCD from phage destruction to DNA repair

  2019cites this paper
• DNA replication and replication termination in Escherichia coli

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• Bacterial RecA Protein Promotes Adenoviral Recombination during In Vitro Infection

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  2018cites this paper
• Functional fine-tuning between bacterial DNA recombination initiation and quality control systems

  2018cites this paper
• Communication between DNA and nucleotide binding sites facilitates stepping by the RecBCD helicase

  2017cites this paper
• Auxiliary ATP binding sites power rapid unwinding by RecBCD

  2017cites this paper
• Ordering the mob: Insights into replicon and MOB typing schemes from analysis of a curated dataset of publicly available plasmids

  2017cites this paper
• RecA filament maintains structural integrity using ATP-driven internal dynamics

  2017cites this paper
• RecBCD thermodynamic coupling between the nucleotide and DNA states

  2017cites this paper
• Implication of RuvABC and RecG in homologous recombination in Streptomyces ambofaciens.

  2017cites this paper
• Molecular characterization of the IgE-binding epitopes in the fast ω-gliadins of Triticeae in relation to wheat-dependent, exercise-induced anaphylaxis.

  2016cites this paper
• RecBCD Enzyme “Chi Recognition” Mutants Recognize Chi Recombination Hotspots in the Right DNA Context

  2016cites this paper
• Unexpected DNA context-dependence identifies a new determinant of Chi recombination hotspots

  2016cites this paper
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  2015cites this paper
• DNA binding proteins of the phage λ ninR region

  2015cites this paper
• An Overview of the Molecular Mechanisms of Recombinational DNA Repair.

  2015cites this paper
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  2014cites this paper
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  2014cites this paper
• Genomic Rearrangements and Evolution

  2013cites this paper
• DNA Unwinding Heterogeneity by RecBCD Results from Static Molecules Able to Equilibrate

  2013cites this paper
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  2013cites this paper
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  2012cites this paper
• How RecBCD Enzyme and Chi Promote DNA Break Repair and Recombination: a Molecular Biologist's View

  2012cites this paper
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  2012cites this paper
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  2012cites this paper
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  2012cites this paper
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  2011cites this paper
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  2011cites this paper
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  2011cites this paper
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  2009cites this paper
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  2009cites this paper
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  2009cites this paper
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  2009cites this paper
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  2008cites this paper
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  2008cites this paper
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  2008cites this paper
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  2008cites this paper
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  2007cites this paper
• Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.

  2007cites this paper
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  2007cites this paper
• Validating the significance of genomic properties of Chi sites from the distribution of all octamers in Escherichia coli.

  2007cites this paper
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  2007cites this paper
• The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition.

  2007cites this paper
• Inhibition of RecBCD enzyme by antineoplastic DNA alkylating agents.

  2006cites this paper
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  2005cites this paper
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  2005cites this paper
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  2005cites this paper
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  2005cites this paper
• Identification of oligonucleotide sequences that direct the movement of the Escherichia coli FtsK translocase.

  2005cites this paper
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  2004cites this paper
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  2004cites this paper
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  2004cites this paper
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  2004cites this paper
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  2004cites this paper
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  2003cites this paper
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  2003cites this paper
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  2003cites this paper
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  2002cites this paper
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  2001cites this paper
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  2001cites this paper
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  2000cites this paper
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  2000cites this paper
• Orientation specificity of the Lactococcus lactis Chi site

  2000influential citation
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  2000cites this paper
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  1999cites this paper
• The RecBC enzyme loads RecA protein onto ssDNA asymmetrically and independently of chi, resulting in constitutive recombination activation.

  1999cites this paper
• Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda.

  1999influential citation
• A Single Mutation, RecBD1080A, Eliminates RecA Protein Loading but Not Chi Recognition by RecBCD Enzyme*

  1999cites this paper
• Thermostable Flap Endonuclease from the Archaeon,Pyrococcus horikoshii, Cleaves the Replication Fork-like Structure Endo/Exonucleolytically*

  1999cites this paper
• Regulation of homologous recombination: Chi inactivates RecBCD enzyme by disassembly of the three subunits.

  1999cites this paper
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  1998cites this paper
• DNA strand exchange proteins: a biochemical and physical comparison.

  1998cites this paper
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  1998cites this paper
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  1998cites this paper
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  1998cites this paper