Micro-PIXE mapping of elemental distribution in arbuscular mycorrhizal roots of the grass, Cynodon dactylon, from gold and uranium mine tailings

Abstract A combination of PIXE, proton back-scattering (BS) spectrometry and confocal laser scanning microscopy (CLSM) was used to determine in situ elemental concentrations in arbuscular mycorrhizal (AM) grass roots and AM fungal spores from gold and uranium mine tailings in South Africa. AM regions of roots were characterised by locally elevated P and vesicles were defined by distinctive transition metal and radionuclide distributions. Vesicles (AM structures responsible for nutrient storage), accumulated Mn, Cu, Ni and U, whereas Fe and Zn were present at lower levels than in host tissue. AM spores from mine tailings accumulated Ca, Cr, Fe, Ni, Cu, Br, Y, Th and U, but were deficient in P and K. The sequestration of excess metals and radionuclides in vesicles may limit metal availability, and thus toxicity, to the host.

• Publication year

  1999

• Venue

  Nuclear Instruments & Methods in Physics Research Section B-beam Interactions With Materials and Atoms

• Publication date

  1999-09-02

• Fields of study

  Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/S0168-583X(99)00467-X
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• Sequestration of excess metals and radionuclides in vesicles may limit metal availability and toxicity to the host.

  Confidence 0.84

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• Arbuscular mycorrhizal fungal spores accumulated Ca, Cr, Fe, Ni, Cu, Br, Y, Th, and U but were deficient in P and K.

  Confidence 0.96

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• Regions of arbuscular mycorrhizal grass roots had locally elevated P, and vesicles accumulated Mn, Cu, Ni, and U while Fe and Zn remained below host tissue levels.

  Confidence 0.96

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• Micro-PIXE mapping, proton back-scattering spectrometry, and confocal laser scanning microscopy were used to determine in situ elemental concentrations in arbuscular mycorrhizal grass roots and arbuscular mycorrhizal fungal spores from gold and uranium mine tailings in South Africa.

  Confidence 0.99

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review

• arbuscular mycorrhizal fungal spores

  sample

  Spores produced by arbuscular mycorrhizal fungi.

  Aliases: AM fungal spores, AM spores

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• arbuscular mycorrhizal grass roots

  sample

  Grass root tissue colonized by arbuscular mycorrhizal fungi.

  Aliases: AM grass roots, AM roots

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• confocal laser scanning microscopy

  method

  An optical sectioning microscopy technique for collecting focused image planes.

  Aliases: CLSM

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• elemental concentrations

  measurement

  The measured levels of elements across the samples.

  Aliases: elemental distribution

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• gold and uranium mine tailings

  material

  Mine waste from gold and uranium extraction sites in South Africa.

  Aliases: mine tailings

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• micro-pixe mapping

  method

  A microbeam particle-induced X-ray emission imaging method for mapping elemental concentrations.

  Aliases: micro-PIXE, PIXE mapping

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• proton back-scattering spectrometry

  method

  An ion-beam analysis technique that estimates composition from backscattered protons.

  Aliases: BS spectrometry, proton back-scattering (BS) spectrometry

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review
• vesicles

  structure

  Nutrient-storage structures inside arbuscular mycorrhizal fungi.

  뀨 (7c402c1b98) extractionAll you need is Python (5d7gwfm5zu) reviewmexicorea (qjvnbu8xg3) review

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