Purification of human monocytes on microexudate-coated surfaces.

S. Ackerman,S. Douglas

Published 1978 in Journal of Immunology

ABSTRACT

Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3mM) rapidly and completely removes monocytes, providing a simple technique for preparation of human monocytes in suspension. The method should be valuable for studies of monocyte function where monocyte monolayers are not suitable.

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