G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

• Publication year

  2015

• Venue

  Nature Methods

• Publication date

  2015-04-27

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/nmeth.3370PMID 25915121
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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