Carbon monoxide (CO) is an endogenous gaseous molecule in organisms. Despite its reputation as a lethal gas, recent studies have shown that it is one of the most essential cellular components regulating a variety of biological processes. However, whether CO regulates physiological processes of morphological or developmental patterns in plants is largely unknown. In this paper, the observation that exogenous CO was able to promote the formation of tomato lateral roots (LR) is described. The CO stimulation of LR development was supported by analysis of tomato haem oxygenase-1 (LeHO-1), an enzymatic source of intracellular CO. It is shown that the amount of LeHO-1 proteins and transcripts increased parallel to the LR development. In addition, LeHO-1 loss-of-function tomato mutant yg-2 showed a phenotype of impaired LR development. The phenotype of yg-2 could be restored by treatment with CO. Since auxin is required for LR initiation and NO is shown to be a mediator for LR development, the correlation of CO with auxin and NO was tested. Our analysis revealed that the action of CO was blocked by the auxin transport inhibitor N-1-naphthylphthalamic acid and the NO scavenger cPTIO, respectively. Furthermore, the whole seedling assays of IAA show that treatment with CO increased the overall IAA levels in various tissues of tomato. Exposure of tomato roots to CO also enhanced intracellular NO generation. These results indicate that CO plays a critical role in controlling architectural change in tomato roots.
Regulation of tomato lateral root development by carbon monoxide and involvement in auxin and nitric oxide
Published 2008 in Journal of Experimental Botany
ABSTRACT
PUBLICATION RECORD
- Publication year
2008
- Venue
Journal of Experimental Botany
- Publication date
2008-07-24
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- auxin
A plant hormone involved in root initiation and transport in the assays described in the abstract.
Aliases: IAA
- carbon monoxide
A gaseous small molecule examined here as an exogenous treatment and as a possible endogenous signal in tomato roots.
Aliases: CO
- cptio
A nitric oxide scavenger used to test whether nitric oxide is required for the carbon monoxide response.
Aliases: 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide
- haem oxygenase-1 (leho-1)
The tomato haem oxygenase-1 enzyme proposed here as an intracellular source of carbon monoxide.
Aliases: LeHO-1, heme oxygenase-1
- iaa
Indole-3-acetic acid, the main auxin form whose overall levels were measured across tomato tissues after carbon monoxide exposure.
Aliases: indole-3-acetic acid
- n-1-naphthylphthalamic acid
An inhibitor of auxin transport used to test whether auxin movement is required for the carbon monoxide response.
Aliases: NPA
- nitric oxide
A reactive signaling molecule measured in tomato roots in connection with carbon monoxide treatment.
Aliases: NO
- tomato lateral root development
The formation and growth of lateral roots in tomato seedlings that serves as the developmental outcome in the abstract.
Aliases: LR development, lateral root development
- yg-2 mutant
A tomato loss-of-function mutant for LeHO-1 used to assess the role of endogenous carbon monoxide in root development.
Aliases: yg-2
REFERENCES
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